Experiment: BG11 with nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpsB and tsf are separated by 102 nucleotides tsf and Synpcc7942_2532 are separated by 83 nucleotides Synpcc7942_2532 and Synpcc7942_2533 are separated by 108 nucleotides Synpcc7942_2533 and Synpcc7942_2534 are separated by 16 nucleotides
Synpcc7942_2530: rpsB - 30S ribosomal protein S2, at 2,611,383 to 2,612,138
rpsB
Synpcc7942_2531: tsf - elongation factor Ts, at 2,612,241 to 2,612,906
tsf
Synpcc7942_2532: Synpcc7942_2532 - hypothetical protein, at 2,612,990 to 2,613,889
_2532
Synpcc7942_2533: Synpcc7942_2533 - hypothetical protein, at 2,613,998 to 2,614,378
_2533
Synpcc7942_2534: Synpcc7942_2534 - diguanylate cyclase with GAF sensor, at 2,614,395 to 2,615,660
_2534
Position (kb)
2612
2613
2614 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2612.162 kb on - strand at 2612.167 kb on - strand at 2612.951 kb on - strand at 2613.017 kb on + strand at 2613.022 kb on + strand at 2613.022 kb on + strand at 2613.022 kb on + strand at 2613.030 kb on - strand at 2613.030 kb on - strand at 2613.030 kb on - strand at 2613.030 kb on - strand at 2613.040 kb on - strand at 2613.105 kb on + strand, within Synpcc7942_2532 at 2613.131 kb on - strand, within Synpcc7942_2532 at 2613.148 kb on - strand, within Synpcc7942_2532 at 2613.161 kb on + strand, within Synpcc7942_2532 at 2613.179 kb on + strand, within Synpcc7942_2532 at 2613.179 kb on + strand, within Synpcc7942_2532 at 2613.180 kb on + strand, within Synpcc7942_2532 at 2613.181 kb on - strand, within Synpcc7942_2532 at 2613.205 kb on - strand, within Synpcc7942_2532 at 2613.205 kb on - strand, within Synpcc7942_2532 at 2613.205 kb on - strand, within Synpcc7942_2532 at 2613.230 kb on + strand, within Synpcc7942_2532 at 2613.230 kb on + strand, within Synpcc7942_2532 at 2613.230 kb on + strand, within Synpcc7942_2532 at 2613.230 kb on + strand, within Synpcc7942_2532 at 2613.238 kb on - strand, within Synpcc7942_2532 at 2613.238 kb on - strand, within Synpcc7942_2532 at 2613.238 kb on - strand, within Synpcc7942_2532 at 2613.238 kb on - strand, within Synpcc7942_2532 at 2613.262 kb on + strand, within Synpcc7942_2532 at 2613.262 kb on + strand, within Synpcc7942_2532 at 2613.262 kb on + strand, within Synpcc7942_2532 at 2613.270 kb on - strand, within Synpcc7942_2532 at 2613.336 kb on + strand, within Synpcc7942_2532 at 2613.336 kb on + strand, within Synpcc7942_2532 at 2613.344 kb on - strand, within Synpcc7942_2532 at 2613.346 kb on + strand, within Synpcc7942_2532 at 2613.354 kb on - strand, within Synpcc7942_2532 at 2613.413 kb on - strand, within Synpcc7942_2532 at 2613.459 kb on + strand, within Synpcc7942_2532 at 2613.459 kb on + strand, within Synpcc7942_2532 at 2613.467 kb on - strand at 2613.501 kb on + strand, within Synpcc7942_2532 at 2613.504 kb on - strand, within Synpcc7942_2532 at 2613.509 kb on + strand, within Synpcc7942_2532 at 2613.509 kb on - strand, within Synpcc7942_2532 at 2613.517 kb on + strand, within Synpcc7942_2532 at 2613.522 kb on - strand, within Synpcc7942_2532 at 2613.527 kb on - strand, within Synpcc7942_2532 at 2613.529 kb on - strand, within Synpcc7942_2532 at 2613.557 kb on - strand, within Synpcc7942_2532 at 2613.561 kb on + strand, within Synpcc7942_2532 at 2613.573 kb on + strand, within Synpcc7942_2532 at 2613.573 kb on + strand at 2613.649 kb on + strand, within Synpcc7942_2532 at 2613.655 kb on - strand, within Synpcc7942_2532 at 2613.685 kb on + strand, within Synpcc7942_2532 at 2613.693 kb on - strand, within Synpcc7942_2532 at 2613.693 kb on - strand, within Synpcc7942_2532 at 2613.694 kb on - strand, within Synpcc7942_2532 at 2613.700 kb on + strand, within Synpcc7942_2532 at 2613.734 kb on - strand, within Synpcc7942_2532 at 2613.763 kb on - strand, within Synpcc7942_2532 at 2613.783 kb on + strand, within Synpcc7942_2532 at 2613.784 kb on - strand, within Synpcc7942_2532 at 2613.796 kb on - strand, within Synpcc7942_2532 at 2613.796 kb on - strand, within Synpcc7942_2532 at 2613.796 kb on - strand, within Synpcc7942_2532 at 2613.815 kb on + strand at 2613.823 kb on - strand at 2613.831 kb on + strand at 2613.831 kb on + strand at 2613.855 kb on + strand at 2613.855 kb on + strand at 2613.855 kb on + strand at 2613.855 kb on + strand at 2613.855 kb on + strand at 2613.863 kb on - strand at 2613.863 kb on - strand at 2613.885 kb on + strand at 2613.885 kb on + strand at 2613.890 kb on + strand at 2613.892 kb on + strand at 2613.921 kb on - strand at 2613.948 kb on + strand at 2613.948 kb on + strand at 2613.956 kb on - strand at 2613.956 kb on - strand at 2613.956 kb on - strand at 2613.968 kb on - strand at 2613.968 kb on - strand at 2614.070 kb on - strand, within Synpcc7942_2533 at 2614.075 kb on + strand, within Synpcc7942_2533 at 2614.078 kb on - strand, within Synpcc7942_2533 at 2614.140 kb on - strand, within Synpcc7942_2533 at 2614.210 kb on + strand, within Synpcc7942_2533 at 2614.233 kb on + strand, within Synpcc7942_2533 at 2614.234 kb on - strand, within Synpcc7942_2533 at 2614.238 kb on - strand, within Synpcc7942_2533 at 2614.272 kb on + strand, within Synpcc7942_2533 at 2614.284 kb on + strand, within Synpcc7942_2533 at 2614.284 kb on + strand, within Synpcc7942_2533 at 2614.284 kb on + strand, within Synpcc7942_2533 at 2614.284 kb on + strand, within Synpcc7942_2533 at 2614.284 kb on + strand, within Synpcc7942_2533 at 2614.289 kb on + strand, within Synpcc7942_2533 at 2614.292 kb on - strand, within Synpcc7942_2533 at 2614.292 kb on - strand, within Synpcc7942_2533 at 2614.292 kb on - strand, within Synpcc7942_2533 at 2614.292 kb on - strand, within Synpcc7942_2533 at 2614.294 kb on + strand, within Synpcc7942_2533 at 2614.297 kb on - strand, within Synpcc7942_2533 at 2614.297 kb on - strand, within Synpcc7942_2533 at 2614.340 kb on + strand, within Synpcc7942_2533 at 2614.348 kb on - strand at 2614.375 kb on - strand at 2614.449 kb on + strand at 2614.473 kb on + strand at 2614.493 kb on + strand at 2614.589 kb on - strand, within Synpcc7942_2534 at 2614.624 kb on - strand, within Synpcc7942_2534 at 2614.673 kb on - strand, within Synpcc7942_2534 at 2614.690 kb on - strand, within Synpcc7942_2534 at 2614.806 kb on + strand, within Synpcc7942_2534 at 2614.813 kb on + strand, within Synpcc7942_2534 at 2614.814 kb on - strand, within Synpcc7942_2534 at 2614.846 kb on - strand, within Synpcc7942_2534
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with nitrite 20 mM remove 2,612,162 - +0.7 2,612,167 - +0.4 2,612,951 - +1.1 2,613,017 + -0.5 2,613,022 + -0.1 2,613,022 + +0.1 2,613,022 + +0.5 2,613,030 - +0.3 2,613,030 - +1.0 2,613,030 - +0.9 2,613,030 - +0.8 2,613,040 - +0.4 2,613,105 + Synpcc7942_2532 0.13 +0.6 2,613,131 - Synpcc7942_2532 0.16 -0.0 2,613,148 - Synpcc7942_2532 0.18 +0.3 2,613,161 + Synpcc7942_2532 0.19 -1.7 2,613,179 + Synpcc7942_2532 0.21 -0.7 2,613,179 + Synpcc7942_2532 0.21 -0.6 2,613,180 + Synpcc7942_2532 0.21 +0.5 2,613,181 - Synpcc7942_2532 0.21 +0.3 2,613,205 - Synpcc7942_2532 0.24 -0.2 2,613,205 - Synpcc7942_2532 0.24 +1.8 2,613,205 - Synpcc7942_2532 0.24 +0.3 2,613,230 + Synpcc7942_2532 0.27 +1.6 2,613,230 + Synpcc7942_2532 0.27 -1.4 2,613,230 + Synpcc7942_2532 0.27 +0.5 2,613,230 + Synpcc7942_2532 0.27 +0.8 2,613,238 - Synpcc7942_2532 0.28 +0.9 2,613,238 - Synpcc7942_2532 0.28 -0.8 2,613,238 - Synpcc7942_2532 0.28 +1.5 2,613,238 - Synpcc7942_2532 0.28 +2.5 2,613,262 + Synpcc7942_2532 0.30 +0.2 2,613,262 + Synpcc7942_2532 0.30 +3.8 2,613,262 + Synpcc7942_2532 0.30 -0.7 2,613,270 - Synpcc7942_2532 0.31 +0.1 2,613,336 + Synpcc7942_2532 0.38 -0.1 2,613,336 + Synpcc7942_2532 0.38 +0.0 2,613,344 - Synpcc7942_2532 0.39 -1.1 2,613,346 + Synpcc7942_2532 0.40 -0.6 2,613,354 - Synpcc7942_2532 0.40 +0.1 2,613,413 - Synpcc7942_2532 0.47 -2.5 2,613,459 + Synpcc7942_2532 0.52 -0.9 2,613,459 + Synpcc7942_2532 0.52 +0.0 2,613,467 - +0.0 2,613,501 + Synpcc7942_2532 0.57 +0.3 2,613,504 - Synpcc7942_2532 0.57 -0.0 2,613,509 + Synpcc7942_2532 0.58 -1.1 2,613,509 - Synpcc7942_2532 0.58 +1.2 2,613,517 + Synpcc7942_2532 0.59 -0.1 2,613,522 - Synpcc7942_2532 0.59 +0.4 2,613,527 - Synpcc7942_2532 0.60 -0.9 2,613,529 - Synpcc7942_2532 0.60 -0.9 2,613,557 - Synpcc7942_2532 0.63 -0.4 2,613,561 + Synpcc7942_2532 0.63 +0.6 2,613,573 + Synpcc7942_2532 0.65 -0.9 2,613,573 + +2.3 2,613,649 + Synpcc7942_2532 0.73 -0.1 2,613,655 - Synpcc7942_2532 0.74 -1.6 2,613,685 + Synpcc7942_2532 0.77 -0.5 2,613,693 - Synpcc7942_2532 0.78 -0.9 2,613,693 - Synpcc7942_2532 0.78 +0.3 2,613,694 - Synpcc7942_2532 0.78 -0.6 2,613,700 + Synpcc7942_2532 0.79 -0.2 2,613,734 - Synpcc7942_2532 0.83 +0.3 2,613,763 - Synpcc7942_2532 0.86 -1.5 2,613,783 + Synpcc7942_2532 0.88 +0.9 2,613,784 - Synpcc7942_2532 0.88 +0.3 2,613,796 - Synpcc7942_2532 0.90 -0.3 2,613,796 - Synpcc7942_2532 0.90 +0.3 2,613,796 - Synpcc7942_2532 0.90 +0.3 2,613,815 + +0.3 2,613,823 - -0.7 2,613,831 + +0.3 2,613,831 + +0.2 2,613,855 + +0.4 2,613,855 + -0.6 2,613,855 + -1.2 2,613,855 + -0.7 2,613,855 + -1.3 2,613,863 - +0.5 2,613,863 - -0.3 2,613,885 + +0.1 2,613,885 + -0.5 2,613,890 + +0.6 2,613,892 + -0.4 2,613,921 - +0.4 2,613,948 + -2.2 2,613,948 + +0.2 2,613,956 - +0.1 2,613,956 - -0.1 2,613,956 - +0.6 2,613,968 - -0.6 2,613,968 - -1.6 2,614,070 - Synpcc7942_2533 0.19 +0.4 2,614,075 + Synpcc7942_2533 0.20 -0.7 2,614,078 - Synpcc7942_2533 0.21 -0.5 2,614,140 - Synpcc7942_2533 0.37 -1.7 2,614,210 + Synpcc7942_2533 0.56 +0.2 2,614,233 + Synpcc7942_2533 0.62 -1.2 2,614,234 - Synpcc7942_2533 0.62 -1.5 2,614,238 - Synpcc7942_2533 0.63 +0.9 2,614,272 + Synpcc7942_2533 0.72 -0.1 2,614,284 + Synpcc7942_2533 0.75 -0.4 2,614,284 + Synpcc7942_2533 0.75 -0.4 2,614,284 + Synpcc7942_2533 0.75 -0.8 2,614,284 + Synpcc7942_2533 0.75 -0.7 2,614,284 + Synpcc7942_2533 0.75 +0.8 2,614,289 + Synpcc7942_2533 0.76 +0.1 2,614,292 - Synpcc7942_2533 0.77 +0.3 2,614,292 - Synpcc7942_2533 0.77 -1.0 2,614,292 - Synpcc7942_2533 0.77 +1.8 2,614,292 - Synpcc7942_2533 0.77 +0.8 2,614,294 + Synpcc7942_2533 0.78 -0.2 2,614,297 - Synpcc7942_2533 0.78 -1.0 2,614,297 - Synpcc7942_2533 0.78 -0.5 2,614,340 + Synpcc7942_2533 0.90 -1.1 2,614,348 - -0.2 2,614,375 - +0.8 2,614,449 + +0.7 2,614,473 + -0.0 2,614,493 + -0.7 2,614,589 - Synpcc7942_2534 0.15 +0.0 2,614,624 - Synpcc7942_2534 0.18 -1.7 2,614,673 - Synpcc7942_2534 0.22 -0.6 2,614,690 - Synpcc7942_2534 0.23 -0.2 2,614,806 + Synpcc7942_2534 0.32 +0.6 2,614,813 + Synpcc7942_2534 0.33 -0.4 2,614,814 - Synpcc7942_2534 0.33 -1.4 2,614,846 - Synpcc7942_2534 0.36 -1.3
Or see this region's nucleotide sequence