Experiment: Zinc chloride 0.0000015625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1641 and Pf6N2E2_1642 are separated by 3 nucleotides Pf6N2E2_1642 and Pf6N2E2_1643 are separated by 96 nucleotides
Pf6N2E2_1641 - Chemotaxis protein CheC -- inhibitor of MCP methylation, at 1,944,672 to 1,945,670
_1641
Pf6N2E2_1642 - diguanylate cyclase (GGDEF domain) with PAS/PAC sensor, at 1,945,674 to 1,946,627
_1642
Pf6N2E2_1643 - Trehalase (EC 3.2.1.28); Periplasmic trehalase precursor (EC 3.2.1.28), at 1,946,724 to 1,948,367
_1643
Position (kb)
1945
1946
1947 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1944.803 kb on - strand, within Pf6N2E2_1641 at 1945.132 kb on + strand, within Pf6N2E2_1641 at 1945.162 kb on + strand, within Pf6N2E2_1641 at 1945.162 kb on + strand, within Pf6N2E2_1641 at 1945.163 kb on - strand, within Pf6N2E2_1641 at 1945.319 kb on - strand, within Pf6N2E2_1641 at 1945.570 kb on - strand, within Pf6N2E2_1641 at 1945.570 kb on - strand, within Pf6N2E2_1641 at 1945.657 kb on - strand at 1945.764 kb on + strand at 1945.966 kb on - strand, within Pf6N2E2_1642 at 1945.966 kb on - strand, within Pf6N2E2_1642 at 1945.999 kb on - strand, within Pf6N2E2_1642 at 1946.067 kb on + strand, within Pf6N2E2_1642 at 1946.067 kb on + strand, within Pf6N2E2_1642 at 1946.067 kb on + strand, within Pf6N2E2_1642 at 1946.067 kb on + strand, within Pf6N2E2_1642 at 1946.068 kb on - strand, within Pf6N2E2_1642 at 1946.068 kb on - strand, within Pf6N2E2_1642 at 1946.131 kb on + strand, within Pf6N2E2_1642 at 1946.132 kb on - strand, within Pf6N2E2_1642 at 1946.132 kb on - strand, within Pf6N2E2_1642 at 1946.283 kb on + strand, within Pf6N2E2_1642 at 1946.284 kb on - strand, within Pf6N2E2_1642 at 1946.284 kb on - strand, within Pf6N2E2_1642 at 1946.284 kb on - strand, within Pf6N2E2_1642 at 1946.284 kb on - strand, within Pf6N2E2_1642 at 1946.475 kb on + strand, within Pf6N2E2_1642 at 1946.475 kb on + strand, within Pf6N2E2_1642 at 1946.475 kb on + strand, within Pf6N2E2_1642 at 1946.475 kb on + strand, within Pf6N2E2_1642 at 1946.476 kb on - strand, within Pf6N2E2_1642 at 1946.476 kb on - strand, within Pf6N2E2_1642 at 1946.580 kb on + strand at 1946.580 kb on + strand at 1946.583 kb on - strand at 1946.583 kb on - strand at 1946.625 kb on + strand at 1946.625 kb on + strand at 1946.626 kb on - strand at 1946.730 kb on - strand at 1946.759 kb on - strand at 1946.818 kb on + strand at 1946.818 kb on + strand at 1946.818 kb on + strand at 1946.818 kb on + strand at 1946.819 kb on - strand at 1946.819 kb on - strand at 1946.861 kb on - strand at 1946.861 kb on - strand at 1946.861 kb on - strand at 1947.076 kb on - strand, within Pf6N2E2_1643 at 1947.125 kb on - strand, within Pf6N2E2_1643 at 1947.185 kb on - strand, within Pf6N2E2_1643 at 1947.194 kb on - strand, within Pf6N2E2_1643 at 1947.194 kb on - strand, within Pf6N2E2_1643 at 1947.328 kb on + strand, within Pf6N2E2_1643 at 1947.372 kb on + strand, within Pf6N2E2_1643 at 1947.372 kb on + strand, within Pf6N2E2_1643 at 1947.373 kb on - strand, within Pf6N2E2_1643 at 1947.373 kb on - strand, within Pf6N2E2_1643
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M remove 1,944,803 - Pf6N2E2_1641 0.13 -0.8 1,945,132 + Pf6N2E2_1641 0.46 +2.6 1,945,162 + Pf6N2E2_1641 0.49 +0.1 1,945,162 + Pf6N2E2_1641 0.49 -0.8 1,945,163 - Pf6N2E2_1641 0.49 +1.5 1,945,319 - Pf6N2E2_1641 0.65 +0.0 1,945,570 - Pf6N2E2_1641 0.90 +2.0 1,945,570 - Pf6N2E2_1641 0.90 -0.8 1,945,657 - -1.9 1,945,764 + -0.1 1,945,966 - Pf6N2E2_1642 0.31 +1.1 1,945,966 - Pf6N2E2_1642 0.31 -1.5 1,945,999 - Pf6N2E2_1642 0.34 +1.4 1,946,067 + Pf6N2E2_1642 0.41 +1.5 1,946,067 + Pf6N2E2_1642 0.41 +1.5 1,946,067 + Pf6N2E2_1642 0.41 -2.3 1,946,067 + Pf6N2E2_1642 0.41 +2.3 1,946,068 - Pf6N2E2_1642 0.41 +1.2 1,946,068 - Pf6N2E2_1642 0.41 -0.5 1,946,131 + Pf6N2E2_1642 0.48 +0.7 1,946,132 - Pf6N2E2_1642 0.48 -0.3 1,946,132 - Pf6N2E2_1642 0.48 -1.3 1,946,283 + Pf6N2E2_1642 0.64 +1.2 1,946,284 - Pf6N2E2_1642 0.64 -1.1 1,946,284 - Pf6N2E2_1642 0.64 -0.3 1,946,284 - Pf6N2E2_1642 0.64 -2.7 1,946,284 - Pf6N2E2_1642 0.64 -0.1 1,946,475 + Pf6N2E2_1642 0.84 +1.4 1,946,475 + Pf6N2E2_1642 0.84 +0.8 1,946,475 + Pf6N2E2_1642 0.84 +1.9 1,946,475 + Pf6N2E2_1642 0.84 -1.4 1,946,476 - Pf6N2E2_1642 0.84 -0.3 1,946,476 - Pf6N2E2_1642 0.84 +1.0 1,946,580 + -1.0 1,946,580 + -0.2 1,946,583 - +0.9 1,946,583 - -1.3 1,946,625 + -1.5 1,946,625 + +0.0 1,946,626 - +0.5 1,946,730 - +0.4 1,946,759 - +0.5 1,946,818 + -1.3 1,946,818 + -0.4 1,946,818 + -2.3 1,946,818 + +0.8 1,946,819 - -1.9 1,946,819 - +1.1 1,946,861 - -0.3 1,946,861 - +1.9 1,946,861 - -2.1 1,947,076 - Pf6N2E2_1643 0.21 -0.7 1,947,125 - Pf6N2E2_1643 0.24 -1.1 1,947,185 - Pf6N2E2_1643 0.28 +0.6 1,947,194 - Pf6N2E2_1643 0.29 +0.6 1,947,194 - Pf6N2E2_1643 0.29 -2.5 1,947,328 + Pf6N2E2_1643 0.37 +2.3 1,947,372 + Pf6N2E2_1643 0.39 +1.2 1,947,372 + Pf6N2E2_1643 0.39 -1.5 1,947,373 - Pf6N2E2_1643 0.39 -0.1 1,947,373 - Pf6N2E2_1643 0.39 +1.0
Or see this region's nucleotide sequence