Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_489 and Pf6N2E2_490 are separated by 173 nucleotides Pf6N2E2_490 and Pf6N2E2_491 are separated by 788 nucleotides
Pf6N2E2_489 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 555,397 to 556,218
_489
Pf6N2E2_490 - PhaK-like protein, at 556,392 to 557,693
_490
Pf6N2E2_491 - hypothetical protein, at 558,482 to 558,835
_491
Position (kb)
556
557
558 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 555.440 kb on + strand at 555.584 kb on + strand, within Pf6N2E2_489 at 555.584 kb on + strand, within Pf6N2E2_489 at 555.615 kb on - strand, within Pf6N2E2_489 at 555.747 kb on - strand, within Pf6N2E2_489 at 555.770 kb on + strand, within Pf6N2E2_489 at 555.770 kb on + strand, within Pf6N2E2_489 at 555.771 kb on - strand, within Pf6N2E2_489 at 555.771 kb on - strand, within Pf6N2E2_489 at 555.791 kb on + strand, within Pf6N2E2_489 at 555.791 kb on + strand, within Pf6N2E2_489 at 555.791 kb on + strand, within Pf6N2E2_489 at 555.843 kb on - strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 556.177 kb on + strand at 556.177 kb on + strand at 556.177 kb on + strand at 556.178 kb on - strand at 556.178 kb on - strand at 556.178 kb on - strand at 556.224 kb on + strand at 556.225 kb on - strand at 556.237 kb on + strand at 556.237 kb on + strand at 556.246 kb on + strand at 556.246 kb on + strand at 556.300 kb on + strand at 556.393 kb on + strand at 556.453 kb on + strand at 556.453 kb on + strand at 556.516 kb on - strand at 557.014 kb on + strand, within Pf6N2E2_490 at 557.014 kb on + strand, within Pf6N2E2_490 at 557.380 kb on + strand, within Pf6N2E2_490 at 557.380 kb on + strand, within Pf6N2E2_490 at 557.538 kb on - strand, within Pf6N2E2_490 at 557.694 kb on + strand at 557.694 kb on + strand at 557.759 kb on + strand at 557.803 kb on + strand at 557.803 kb on + strand at 557.949 kb on + strand at 557.968 kb on + strand at 557.968 kb on + strand at 558.118 kb on - strand at 558.154 kb on + strand at 558.154 kb on + strand at 558.154 kb on + strand at 558.154 kb on + strand at 558.155 kb on - strand at 558.155 kb on - strand at 558.175 kb on + strand at 558.175 kb on + strand at 558.176 kb on - strand at 558.176 kb on - strand at 558.176 kb on - strand at 558.176 kb on - strand at 558.197 kb on + strand at 558.197 kb on + strand at 558.198 kb on - strand at 558.198 kb on - strand at 558.198 kb on - strand at 558.231 kb on + strand at 558.232 kb on - strand at 558.245 kb on + strand at 558.246 kb on - strand at 558.246 kb on - strand at 558.306 kb on - strand at 558.397 kb on + strand at 558.397 kb on + strand at 558.409 kb on + strand at 558.410 kb on - strand at 558.423 kb on + strand at 558.424 kb on - strand at 558.424 kb on - strand at 558.458 kb on + strand at 558.458 kb on + strand at 558.458 kb on + strand at 558.459 kb on - strand at 558.459 kb on - strand at 558.459 kb on - strand at 558.461 kb on + strand at 558.461 kb on + strand at 558.461 kb on + strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.481 kb on + strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.578 kb on + strand, within Pf6N2E2_491 at 558.578 kb on + strand, within Pf6N2E2_491 at 558.579 kb on - strand, within Pf6N2E2_491 at 558.579 kb on - strand, within Pf6N2E2_491 at 558.579 kb on - strand, within Pf6N2E2_491 at 558.581 kb on + strand, within Pf6N2E2_491 at 558.581 kb on + strand, within Pf6N2E2_491 at 558.582 kb on - strand, within Pf6N2E2_491 at 558.582 kb on - strand, within Pf6N2E2_491 at 558.582 kb on - strand, within Pf6N2E2_491
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 555,440 + -0.0 555,584 + Pf6N2E2_489 0.23 +0.5 555,584 + Pf6N2E2_489 0.23 -1.6 555,615 - Pf6N2E2_489 0.27 -0.6 555,747 - Pf6N2E2_489 0.43 -1.1 555,770 + Pf6N2E2_489 0.45 +0.9 555,770 + Pf6N2E2_489 0.45 -0.2 555,771 - Pf6N2E2_489 0.45 +0.0 555,771 - Pf6N2E2_489 0.45 -0.4 555,791 + Pf6N2E2_489 0.48 +0.8 555,791 + Pf6N2E2_489 0.48 +0.5 555,791 + Pf6N2E2_489 0.48 -0.2 555,843 - Pf6N2E2_489 0.54 +1.1 555,911 + Pf6N2E2_489 0.63 +0.8 555,911 + Pf6N2E2_489 0.63 -0.0 555,911 + Pf6N2E2_489 0.63 +0.3 555,911 + Pf6N2E2_489 0.63 +0.5 556,177 + -0.7 556,177 + +0.8 556,177 + +1.0 556,178 - -2.3 556,178 - +0.5 556,178 - +0.4 556,224 + -0.2 556,225 - -0.2 556,237 + +0.2 556,237 + -0.7 556,246 + -2.9 556,246 + +0.3 556,300 + +1.0 556,393 + +0.6 556,453 + -0.4 556,453 + -1.1 556,516 - -0.3 557,014 + Pf6N2E2_490 0.48 -0.9 557,014 + Pf6N2E2_490 0.48 -0.1 557,380 + Pf6N2E2_490 0.76 -0.6 557,380 + Pf6N2E2_490 0.76 -2.6 557,538 - Pf6N2E2_490 0.88 +0.7 557,694 + -0.5 557,694 + +0.4 557,759 + -0.6 557,803 + +0.0 557,803 + +0.6 557,949 + -0.3 557,968 + +0.2 557,968 + -1.0 558,118 - -2.1 558,154 + -0.1 558,154 + -0.1 558,154 + -0.5 558,154 + -1.6 558,155 - +0.0 558,155 - +0.1 558,175 + -0.1 558,175 + -1.0 558,176 - -0.8 558,176 - -0.3 558,176 - -0.3 558,176 - -0.8 558,197 + -0.8 558,197 + +0.5 558,198 - +0.1 558,198 - +0.4 558,198 - +0.8 558,231 + -0.4 558,232 - +0.4 558,245 + +2.1 558,246 - -2.0 558,246 - -1.2 558,306 - +0.4 558,397 + -0.6 558,397 + +0.0 558,409 + -0.4 558,410 - +0.0 558,423 + +0.3 558,424 - -0.1 558,424 - -0.8 558,458 + +0.2 558,458 + +0.4 558,458 + -0.2 558,459 - -1.6 558,459 - +0.7 558,459 - +0.2 558,461 + -0.8 558,461 + -0.1 558,461 + +0.6 558,462 - +0.3 558,462 - -0.2 558,462 - +0.9 558,462 - -2.0 558,462 - +1.9 558,481 + -0.1 558,482 - +0.6 558,482 - -0.6 558,482 - +0.9 558,482 - -0.1 558,482 - -0.5 558,482 - +1.0 558,482 - +1.5 558,482 - +0.9 558,482 - +0.5 558,482 - +0.9 558,482 - -0.0 558,578 + Pf6N2E2_491 0.27 -1.8 558,578 + Pf6N2E2_491 0.27 -0.0 558,579 - Pf6N2E2_491 0.27 -0.5 558,579 - Pf6N2E2_491 0.27 +0.5 558,579 - Pf6N2E2_491 0.27 +1.5 558,581 + Pf6N2E2_491 0.28 +0.0 558,581 + Pf6N2E2_491 0.28 -0.0 558,582 - Pf6N2E2_491 0.28 -1.5 558,582 - Pf6N2E2_491 0.28 +0.4 558,582 - Pf6N2E2_491 0.28 -0.5
Or see this region's nucleotide sequence