Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_237

Experiment: Calcium chloride 0.0125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_236 and Pf6N2E2_237 are separated by 291 nucleotidesPf6N2E2_237 and Pf6N2E2_238 are separated by 219 nucleotidesPf6N2E2_238 and Pf6N2E2_239 are separated by 33 nucleotides Pf6N2E2_236 - putative transcriptional regulator protein, at 298,600 to 299,253 _236 Pf6N2E2_237 - hypothetical protein, at 299,545 to 299,757 _237 Pf6N2E2_238 - hypothetical protein, at 299,977 to 300,096 _238 Pf6N2E2_239 - Fatty acid desaturase, at 300,130 to 300,831 _239 Position (kb) 299 300Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 298.553 kb on - strandat 298.716 kb on + strand, within Pf6N2E2_236at 298.822 kb on + strand, within Pf6N2E2_236at 299.089 kb on + strand, within Pf6N2E2_236at 299.090 kb on - strand, within Pf6N2E2_236at 299.116 kb on + strand, within Pf6N2E2_236at 299.129 kb on + strand, within Pf6N2E2_236at 299.129 kb on + strand, within Pf6N2E2_236at 299.129 kb on + strand, within Pf6N2E2_236at 299.130 kb on - strand, within Pf6N2E2_236at 299.130 kb on - strand, within Pf6N2E2_236at 299.165 kb on + strand, within Pf6N2E2_236at 299.272 kb on + strandat 299.272 kb on + strandat 299.301 kb on - strandat 299.307 kb on + strandat 299.392 kb on + strandat 299.393 kb on - strandat 299.413 kb on + strandat 299.414 kb on - strandat 299.491 kb on + strandat 299.491 kb on + strandat 299.491 kb on + strandat 299.492 kb on - strandat 299.492 kb on - strandat 299.492 kb on - strandat 299.492 kb on - strandat 299.492 kb on - strandat 299.492 kb on - strandat 299.492 kb on - strandat 299.492 kb on - strandat 299.492 kb on - strandat 299.538 kb on + strandat 299.539 kb on - strandat 299.539 kb on - strandat 299.539 kb on - strandat 299.544 kb on + strandat 299.544 kb on + strandat 299.544 kb on + strandat 299.544 kb on + strandat 299.545 kb on - strandat 299.545 kb on - strandat 299.591 kb on - strand, within Pf6N2E2_237at 299.591 kb on - strand, within Pf6N2E2_237at 299.591 kb on - strand, within Pf6N2E2_237at 299.591 kb on - strand, within Pf6N2E2_237at 299.747 kb on - strandat 299.786 kb on - strandat 299.810 kb on - strandat 299.810 kb on - strandat 299.810 kb on - strandat 299.810 kb on - strandat 299.810 kb on - strandat 299.813 kb on - strandat 299.813 kb on - strandat 299.871 kb on - strandat 299.880 kb on + strandat 299.880 kb on + strandat 299.880 kb on + strandat 299.881 kb on - strandat 299.881 kb on - strandat 299.881 kb on - strandat 299.896 kb on + strandat 299.978 kb on + strandat 299.979 kb on - strandat 299.992 kb on + strand, within Pf6N2E2_238at 299.992 kb on + strand, within Pf6N2E2_238at 300.010 kb on - strand, within Pf6N2E2_238at 300.172 kb on + strandat 300.183 kb on + strandat 300.184 kb on - strandat 300.184 kb on - strandat 300.295 kb on + strand, within Pf6N2E2_239at 300.295 kb on + strand, within Pf6N2E2_239at 300.296 kb on - strand, within Pf6N2E2_239at 300.296 kb on - strand, within Pf6N2E2_239at 300.297 kb on + strand, within Pf6N2E2_239at 300.297 kb on + strand, within Pf6N2E2_239at 300.297 kb on + strand, within Pf6N2E2_239at 300.298 kb on - strand, within Pf6N2E2_239at 300.298 kb on - strand, within Pf6N2E2_239at 300.298 kb on - strand, within Pf6N2E2_239at 300.298 kb on - strand, within Pf6N2E2_239at 300.298 kb on - strand, within Pf6N2E2_239at 300.298 kb on - strand, within Pf6N2E2_239at 300.298 kb on - strand, within Pf6N2E2_239at 300.482 kb on - strand, within Pf6N2E2_239at 300.482 kb on - strand, within Pf6N2E2_239at 300.506 kb on - strand, within Pf6N2E2_239at 300.506 kb on - strand, within Pf6N2E2_239at 300.559 kb on + strand, within Pf6N2E2_239at 300.595 kb on - strand, within Pf6N2E2_239at 300.595 kb on - strand, within Pf6N2E2_239at 300.707 kb on - strand, within Pf6N2E2_239at 300.707 kb on - strand, within Pf6N2E2_239at 300.709 kb on - strand, within Pf6N2E2_239at 300.749 kb on - strand, within Pf6N2E2_239

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Per-strain Table

Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M
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298,553 - -0.2
298,716 + Pf6N2E2_236 0.18 +0.7
298,822 + Pf6N2E2_236 0.34 -0.3
299,089 + Pf6N2E2_236 0.75 +0.5
299,090 - Pf6N2E2_236 0.75 -1.4
299,116 + Pf6N2E2_236 0.79 -0.1
299,129 + Pf6N2E2_236 0.81 +0.8
299,129 + Pf6N2E2_236 0.81 -0.3
299,129 + Pf6N2E2_236 0.81 -0.8
299,130 - Pf6N2E2_236 0.81 +0.2
299,130 - Pf6N2E2_236 0.81 +0.2
299,165 + Pf6N2E2_236 0.86 -0.1
299,272 + +0.4
299,272 + +0.3
299,301 - +0.5
299,307 + -0.2
299,392 + +0.1
299,393 - +0.5
299,413 + +0.9
299,414 - -0.1
299,491 + -0.8
299,491 + +0.1
299,491 + -1.3
299,492 - +0.6
299,492 - -0.4
299,492 - +0.1
299,492 - -1.0
299,492 - -0.6
299,492 - -0.6
299,492 - -0.1
299,492 - +1.3
299,492 - +1.4
299,538 + +0.2
299,539 - -0.5
299,539 - -0.4
299,539 - -0.1
299,544 + +1.7
299,544 + +0.3
299,544 + -1.2
299,544 + +0.5
299,545 - -2.0
299,545 - -1.3
299,591 - Pf6N2E2_237 0.22 -0.8
299,591 - Pf6N2E2_237 0.22 -0.4
299,591 - Pf6N2E2_237 0.22 -0.7
299,591 - Pf6N2E2_237 0.22 -0.8
299,747 - -1.0
299,786 - -0.3
299,810 - +1.5
299,810 - -0.7
299,810 - -1.1
299,810 - -0.5
299,810 - -0.0
299,813 - -0.6
299,813 - +0.8
299,871 - +1.3
299,880 + +0.5
299,880 + +0.8
299,880 + +0.0
299,881 - -1.0
299,881 - -0.2
299,881 - +0.8
299,896 + +0.8
299,978 + -1.3
299,979 - -0.7
299,992 + Pf6N2E2_238 0.12 +0.3
299,992 + Pf6N2E2_238 0.12 -0.6
300,010 - Pf6N2E2_238 0.28 -2.2
300,172 + -0.1
300,183 + +0.5
300,184 - -0.7
300,184 - +0.5
300,295 + Pf6N2E2_239 0.24 +0.1
300,295 + Pf6N2E2_239 0.24 +0.3
300,296 - Pf6N2E2_239 0.24 -1.9
300,296 - Pf6N2E2_239 0.24 +0.1
300,297 + Pf6N2E2_239 0.24 -0.5
300,297 + Pf6N2E2_239 0.24 -0.4
300,297 + Pf6N2E2_239 0.24 +0.4
300,298 - Pf6N2E2_239 0.24 +0.2
300,298 - Pf6N2E2_239 0.24 +0.2
300,298 - Pf6N2E2_239 0.24 -1.1
300,298 - Pf6N2E2_239 0.24 +0.9
300,298 - Pf6N2E2_239 0.24 -0.2
300,298 - Pf6N2E2_239 0.24 +0.3
300,298 - Pf6N2E2_239 0.24 -1.2
300,482 - Pf6N2E2_239 0.50 +0.7
300,482 - Pf6N2E2_239 0.50 +0.6
300,506 - Pf6N2E2_239 0.54 -3.7
300,506 - Pf6N2E2_239 0.54 +0.5
300,559 + Pf6N2E2_239 0.61 +0.3
300,595 - Pf6N2E2_239 0.66 -0.1
300,595 - Pf6N2E2_239 0.66 +1.9
300,707 - Pf6N2E2_239 0.82 -1.0
300,707 - Pf6N2E2_239 0.82 -0.8
300,709 - Pf6N2E2_239 0.82 +0.4
300,749 - Pf6N2E2_239 0.88 -0.5

Or see this region's nucleotide sequence