Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS21975 and HSERO_RS21980 are separated by 209 nucleotides HSERO_RS21980 and HSERO_RS21985 overlap by 4 nucleotides HSERO_RS21985 and HSERO_RS21990 are separated by 22 nucleotides HSERO_RS21990 and HSERO_RS21995 are separated by 10 nucleotides
HSERO_RS21975: HSERO_RS21975 - peptidase, at 5,027,060 to 5,028,544
_RS21975
HSERO_RS21980: HSERO_RS21980 - membrane protein, at 5,028,754 to 5,030,088
_RS21980
HSERO_RS21985: HSERO_RS21985 - transcriptional regulator, at 5,030,085 to 5,030,741
_RS21985
HSERO_RS21990: HSERO_RS21990 - hypothetical protein, at 5,030,764 to 5,030,958
_RS21990
HSERO_RS21995: HSERO_RS21995 - hypothetical protein, at 5,030,969 to 5,032,129
_RS21995
Position (kb) 5028
5029
5030
5031 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5027.828 kb on - strand, within HSERO_RS21975 at 5027.886 kb on + strand, within HSERO_RS21975 at 5027.914 kb on + strand, within HSERO_RS21975 at 5027.929 kb on - strand, within HSERO_RS21975 at 5028.126 kb on - strand, within HSERO_RS21975 at 5028.319 kb on + strand, within HSERO_RS21975 at 5028.611 kb on + strand at 5028.657 kb on + strand at 5028.657 kb on + strand at 5028.665 kb on - strand at 5028.665 kb on - strand at 5028.666 kb on - strand at 5028.679 kb on + strand at 5028.689 kb on + strand at 5028.769 kb on - strand at 5028.811 kb on - strand at 5028.811 kb on - strand at 5028.908 kb on - strand, within HSERO_RS21980 at 5028.981 kb on + strand, within HSERO_RS21980 at 5029.054 kb on + strand, within HSERO_RS21980 at 5029.133 kb on + strand, within HSERO_RS21980 at 5029.253 kb on + strand, within HSERO_RS21980 at 5029.267 kb on - strand, within HSERO_RS21980 at 5029.298 kb on - strand, within HSERO_RS21980 at 5029.347 kb on - strand, within HSERO_RS21980 at 5029.468 kb on + strand, within HSERO_RS21980 at 5029.667 kb on - strand, within HSERO_RS21980 at 5029.703 kb on + strand, within HSERO_RS21980 at 5029.711 kb on - strand, within HSERO_RS21980 at 5029.998 kb on + strand at 5030.057 kb on - strand at 5030.093 kb on + strand at 5030.238 kb on - strand, within HSERO_RS21985 at 5030.511 kb on + strand, within HSERO_RS21985 at 5030.582 kb on + strand, within HSERO_RS21985 at 5030.613 kb on - strand, within HSERO_RS21985 at 5030.658 kb on + strand, within HSERO_RS21985 at 5030.658 kb on + strand, within HSERO_RS21985 at 5030.666 kb on - strand, within HSERO_RS21985 at 5030.686 kb on + strand at 5030.854 kb on + strand, within HSERO_RS21990 at 5030.967 kb on + strand at 5030.975 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 5,027,828 - HSERO_RS21975 0.52 -0.3 5,027,886 + HSERO_RS21975 0.56 -0.1 5,027,914 + HSERO_RS21975 0.58 +0.1 5,027,929 - HSERO_RS21975 0.59 +0.2 5,028,126 - HSERO_RS21975 0.72 -0.2 5,028,319 + HSERO_RS21975 0.85 +0.1 5,028,611 + +0.4 5,028,657 + -4.4 5,028,657 + -0.0 5,028,665 - +0.4 5,028,665 - -0.4 5,028,666 - -0.2 5,028,679 + -1.4 5,028,689 + -0.5 5,028,769 - +1.1 5,028,811 - -0.3 5,028,811 - -0.4 5,028,908 - HSERO_RS21980 0.12 -1.3 5,028,981 + HSERO_RS21980 0.17 -1.2 5,029,054 + HSERO_RS21980 0.22 -0.8 5,029,133 + HSERO_RS21980 0.28 -0.7 5,029,253 + HSERO_RS21980 0.37 -1.2 5,029,267 - HSERO_RS21980 0.38 -0.7 5,029,298 - HSERO_RS21980 0.41 -0.8 5,029,347 - HSERO_RS21980 0.44 -1.3 5,029,468 + HSERO_RS21980 0.53 -1.5 5,029,667 - HSERO_RS21980 0.68 -0.7 5,029,703 + HSERO_RS21980 0.71 -1.6 5,029,711 - HSERO_RS21980 0.72 -0.9 5,029,998 + -0.6 5,030,057 - -0.5 5,030,093 + +3.2 5,030,238 - HSERO_RS21985 0.23 -0.5 5,030,511 + HSERO_RS21985 0.65 -0.4 5,030,582 + HSERO_RS21985 0.76 -0.5 5,030,613 - HSERO_RS21985 0.80 -1.0 5,030,658 + HSERO_RS21985 0.87 -0.0 5,030,658 + HSERO_RS21985 0.87 +0.2 5,030,666 - HSERO_RS21985 0.88 -0.3 5,030,686 + -0.1 5,030,854 + HSERO_RS21990 0.46 +0.1 5,030,967 + +0.1 5,030,975 - +0.2
Or see this region's nucleotide sequence