Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS14280

Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS14265 and HSERO_RS14270 are separated by 26 nucleotidesHSERO_RS14270 and HSERO_RS14275 are separated by 3 nucleotidesHSERO_RS14275 and HSERO_RS14280 overlap by 4 nucleotidesHSERO_RS14280 and HSERO_RS14285 are separated by 4 nucleotides HSERO_RS14265: HSERO_RS14265 - hypothetical protein, at 3,239,054 to 3,239,260 _RS14265 HSERO_RS14270: HSERO_RS14270 - hypothetical protein, at 3,239,287 to 3,239,757 _RS14270 HSERO_RS14275: HSERO_RS14275 - nitrogen fixation protein NifX, at 3,239,761 to 3,240,162 _RS14275 HSERO_RS14280: HSERO_RS14280 - nitrogenase molybdenum-cofactor synthesis protein NifN protein, at 3,240,159 to 3,241,547 _RS14280 HSERO_RS14285: HSERO_RS14285 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 3,241,552 to 3,242,931 _RS14285 Position (kb) 3240 3241 3242Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3239.294 kb on + strandat 3239.388 kb on - strand, within HSERO_RS14270at 3239.534 kb on + strand, within HSERO_RS14270at 3239.617 kb on + strand, within HSERO_RS14270at 3239.772 kb on - strandat 3239.818 kb on - strand, within HSERO_RS14275at 3239.818 kb on - strand, within HSERO_RS14275at 3239.834 kb on - strandat 3240.181 kb on + strandat 3240.199 kb on - strandat 3240.307 kb on - strand, within HSERO_RS14280at 3240.435 kb on - strand, within HSERO_RS14280at 3240.457 kb on - strand, within HSERO_RS14280at 3240.508 kb on + strand, within HSERO_RS14280at 3240.527 kb on + strand, within HSERO_RS14280at 3240.600 kb on + strand, within HSERO_RS14280at 3240.745 kb on + strand, within HSERO_RS14280at 3240.756 kb on + strand, within HSERO_RS14280at 3240.872 kb on + strand, within HSERO_RS14280at 3241.155 kb on + strand, within HSERO_RS14280at 3241.163 kb on - strand, within HSERO_RS14280at 3241.163 kb on - strand, within HSERO_RS14280at 3241.168 kb on - strand, within HSERO_RS14280at 3241.207 kb on - strand, within HSERO_RS14280at 3241.231 kb on + strand, within HSERO_RS14280at 3241.265 kb on + strand, within HSERO_RS14280at 3241.276 kb on + strand, within HSERO_RS14280at 3241.412 kb on + strandat 3241.414 kb on + strandat 3241.420 kb on - strandat 3241.467 kb on + strandat 3241.475 kb on - strandat 3241.475 kb on - strandat 3241.475 kb on - strandat 3241.475 kb on - strandat 3241.475 kb on - strandat 3241.475 kb on - strandat 3241.600 kb on - strandat 3241.890 kb on + strand, within HSERO_RS14285at 3241.988 kb on + strand, within HSERO_RS14285at 3241.988 kb on + strand, within HSERO_RS14285at 3242.437 kb on - strand, within HSERO_RS14285

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
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3,239,294 + +0.2
3,239,388 - HSERO_RS14270 0.21 +0.2
3,239,534 + HSERO_RS14270 0.52 -0.1
3,239,617 + HSERO_RS14270 0.70 +1.1
3,239,772 - -0.9
3,239,818 - HSERO_RS14275 0.14 -1.3
3,239,818 - HSERO_RS14275 0.14 -0.7
3,239,834 - -1.6
3,240,181 + -1.4
3,240,199 - -0.7
3,240,307 - HSERO_RS14280 0.11 -4.2
3,240,435 - HSERO_RS14280 0.20 -5.1
3,240,457 - HSERO_RS14280 0.21 -4.8
3,240,508 + HSERO_RS14280 0.25 -4.2
3,240,527 + HSERO_RS14280 0.26 -4.1
3,240,600 + HSERO_RS14280 0.32 -5.1
3,240,745 + HSERO_RS14280 0.42 -4.1
3,240,756 + HSERO_RS14280 0.43 -2.4
3,240,872 + HSERO_RS14280 0.51 -0.6
3,241,155 + HSERO_RS14280 0.72 -3.1
3,241,163 - HSERO_RS14280 0.72 -4.3
3,241,163 - HSERO_RS14280 0.72 -2.6
3,241,168 - HSERO_RS14280 0.73 -4.3
3,241,207 - HSERO_RS14280 0.75 -3.7
3,241,231 + HSERO_RS14280 0.77 -3.3
3,241,265 + HSERO_RS14280 0.80 -2.6
3,241,276 + HSERO_RS14280 0.80 -4.0
3,241,412 + -4.8
3,241,414 + -1.4
3,241,420 - -4.6
3,241,467 + -2.5
3,241,475 - -4.1
3,241,475 - -3.9
3,241,475 - -5.2
3,241,475 - -4.2
3,241,475 - -3.3
3,241,475 - -4.4
3,241,600 - -4.6
3,241,890 + HSERO_RS14285 0.24 -4.8
3,241,988 + HSERO_RS14285 0.32 -3.7
3,241,988 + HSERO_RS14285 0.32 -4.6
3,242,437 - HSERO_RS14285 0.64 -2.3

Or see this region's nucleotide sequence