Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14265 and HSERO_RS14270 are separated by 26 nucleotides HSERO_RS14270 and HSERO_RS14275 are separated by 3 nucleotides HSERO_RS14275 and HSERO_RS14280 overlap by 4 nucleotides HSERO_RS14280 and HSERO_RS14285 are separated by 4 nucleotides
HSERO_RS14265: HSERO_RS14265 - hypothetical protein, at 3,239,054 to 3,239,260
_RS14265
HSERO_RS14270: HSERO_RS14270 - hypothetical protein, at 3,239,287 to 3,239,757
_RS14270
HSERO_RS14275: HSERO_RS14275 - nitrogen fixation protein NifX, at 3,239,761 to 3,240,162
_RS14275
HSERO_RS14280: HSERO_RS14280 - nitrogenase molybdenum-cofactor synthesis protein NifN protein, at 3,240,159 to 3,241,547
_RS14280
HSERO_RS14285: HSERO_RS14285 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 3,241,552 to 3,242,931
_RS14285
Position (kb)
3240
3241
3242 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3239.294 kb on + strand at 3239.388 kb on - strand, within HSERO_RS14270 at 3239.534 kb on + strand, within HSERO_RS14270 at 3239.617 kb on + strand, within HSERO_RS14270 at 3239.772 kb on - strand at 3239.818 kb on - strand, within HSERO_RS14275 at 3239.818 kb on - strand, within HSERO_RS14275 at 3239.834 kb on - strand at 3240.181 kb on + strand at 3240.199 kb on - strand at 3240.307 kb on - strand, within HSERO_RS14280 at 3240.435 kb on - strand, within HSERO_RS14280 at 3240.457 kb on - strand, within HSERO_RS14280 at 3240.508 kb on + strand, within HSERO_RS14280 at 3240.527 kb on + strand, within HSERO_RS14280 at 3240.600 kb on + strand, within HSERO_RS14280 at 3240.745 kb on + strand, within HSERO_RS14280 at 3240.756 kb on + strand, within HSERO_RS14280 at 3240.872 kb on + strand, within HSERO_RS14280 at 3241.155 kb on + strand, within HSERO_RS14280 at 3241.163 kb on - strand, within HSERO_RS14280 at 3241.163 kb on - strand, within HSERO_RS14280 at 3241.168 kb on - strand, within HSERO_RS14280 at 3241.207 kb on - strand, within HSERO_RS14280 at 3241.231 kb on + strand, within HSERO_RS14280 at 3241.265 kb on + strand, within HSERO_RS14280 at 3241.276 kb on + strand, within HSERO_RS14280 at 3241.412 kb on + strand at 3241.414 kb on + strand at 3241.420 kb on - strand at 3241.467 kb on + strand at 3241.475 kb on - strand at 3241.475 kb on - strand at 3241.475 kb on - strand at 3241.475 kb on - strand at 3241.475 kb on - strand at 3241.475 kb on - strand at 3241.600 kb on - strand at 3241.890 kb on + strand, within HSERO_RS14285 at 3241.988 kb on + strand, within HSERO_RS14285 at 3241.988 kb on + strand, within HSERO_RS14285 at 3242.437 kb on - strand, within HSERO_RS14285
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,239,294 + +0.2 3,239,388 - HSERO_RS14270 0.21 +0.2 3,239,534 + HSERO_RS14270 0.52 -0.1 3,239,617 + HSERO_RS14270 0.70 +1.1 3,239,772 - -0.9 3,239,818 - HSERO_RS14275 0.14 -1.3 3,239,818 - HSERO_RS14275 0.14 -0.7 3,239,834 - -1.6 3,240,181 + -1.4 3,240,199 - -0.7 3,240,307 - HSERO_RS14280 0.11 -4.2 3,240,435 - HSERO_RS14280 0.20 -5.1 3,240,457 - HSERO_RS14280 0.21 -4.8 3,240,508 + HSERO_RS14280 0.25 -4.2 3,240,527 + HSERO_RS14280 0.26 -4.1 3,240,600 + HSERO_RS14280 0.32 -5.1 3,240,745 + HSERO_RS14280 0.42 -4.1 3,240,756 + HSERO_RS14280 0.43 -2.4 3,240,872 + HSERO_RS14280 0.51 -0.6 3,241,155 + HSERO_RS14280 0.72 -3.1 3,241,163 - HSERO_RS14280 0.72 -4.3 3,241,163 - HSERO_RS14280 0.72 -2.6 3,241,168 - HSERO_RS14280 0.73 -4.3 3,241,207 - HSERO_RS14280 0.75 -3.7 3,241,231 + HSERO_RS14280 0.77 -3.3 3,241,265 + HSERO_RS14280 0.80 -2.6 3,241,276 + HSERO_RS14280 0.80 -4.0 3,241,412 + -4.8 3,241,414 + -1.4 3,241,420 - -4.6 3,241,467 + -2.5 3,241,475 - -4.1 3,241,475 - -3.9 3,241,475 - -5.2 3,241,475 - -4.2 3,241,475 - -3.3 3,241,475 - -4.4 3,241,600 - -4.6 3,241,890 + HSERO_RS14285 0.24 -4.8 3,241,988 + HSERO_RS14285 0.32 -3.7 3,241,988 + HSERO_RS14285 0.32 -4.6 3,242,437 - HSERO_RS14285 0.64 -2.3
Or see this region's nucleotide sequence