Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04285 and HSERO_RS04290 are separated by 239 nucleotides HSERO_RS04290 and HSERO_RS04295 are separated by 966 nucleotides
HSERO_RS04285: HSERO_RS04285 - glycosyl transferase, at 940,346 to 941,356
_RS04285
HSERO_RS04290: HSERO_RS04290 - DNA-binding protein, at 941,596 to 942,048
_RS04290
HSERO_RS04295: HSERO_RS04295 - diguanylate cyclase, at 943,015 to 945,135
_RS04295
Position (kb)
941
942
943 Strain fitness (log2 ratio)
-1
0
1
2 at 940.680 kb on + strand, within HSERO_RS04285 at 940.722 kb on + strand, within HSERO_RS04285 at 940.814 kb on + strand, within HSERO_RS04285 at 940.822 kb on - strand, within HSERO_RS04285 at 940.822 kb on - strand, within HSERO_RS04285 at 940.849 kb on + strand, within HSERO_RS04285 at 940.849 kb on + strand, within HSERO_RS04285 at 940.856 kb on + strand, within HSERO_RS04285 at 940.857 kb on - strand at 940.946 kb on + strand, within HSERO_RS04285 at 940.949 kb on - strand, within HSERO_RS04285 at 940.970 kb on - strand, within HSERO_RS04285 at 941.026 kb on - strand, within HSERO_RS04285 at 941.099 kb on - strand, within HSERO_RS04285 at 941.273 kb on + strand at 941.273 kb on + strand at 941.318 kb on + strand at 941.318 kb on + strand at 941.371 kb on + strand at 941.380 kb on + strand at 941.400 kb on - strand at 941.402 kb on - strand at 941.434 kb on + strand at 941.457 kb on - strand at 941.532 kb on - strand at 941.553 kb on + strand at 941.670 kb on + strand, within HSERO_RS04290 at 941.700 kb on - strand, within HSERO_RS04290 at 941.742 kb on - strand, within HSERO_RS04290 at 941.861 kb on + strand, within HSERO_RS04290 at 941.917 kb on + strand, within HSERO_RS04290 at 941.981 kb on + strand, within HSERO_RS04290 at 942.261 kb on - strand at 942.273 kb on + strand at 942.281 kb on - strand at 942.400 kb on + strand at 942.641 kb on + strand at 942.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 940,680 + HSERO_RS04285 0.33 +0.1 940,722 + HSERO_RS04285 0.37 +0.3 940,814 + HSERO_RS04285 0.46 +0.1 940,822 - HSERO_RS04285 0.47 -0.0 940,822 - HSERO_RS04285 0.47 -0.3 940,849 + HSERO_RS04285 0.50 -0.2 940,849 + HSERO_RS04285 0.50 +0.1 940,856 + HSERO_RS04285 0.50 -0.4 940,857 - -0.5 940,946 + HSERO_RS04285 0.59 +0.3 940,949 - HSERO_RS04285 0.60 -0.3 940,970 - HSERO_RS04285 0.62 -0.2 941,026 - HSERO_RS04285 0.67 -1.6 941,099 - HSERO_RS04285 0.74 +0.2 941,273 + +0.4 941,273 + +0.7 941,318 + +0.2 941,318 + +1.2 941,371 + +0.2 941,380 + +0.3 941,400 - -0.3 941,402 - -1.1 941,434 + +0.6 941,457 - -0.1 941,532 - -0.9 941,553 + -0.4 941,670 + HSERO_RS04290 0.16 +1.6 941,700 - HSERO_RS04290 0.23 +0.9 941,742 - HSERO_RS04290 0.32 +1.3 941,861 + HSERO_RS04290 0.58 +2.1 941,917 + HSERO_RS04290 0.71 +1.4 941,981 + HSERO_RS04290 0.85 +1.1 942,261 - -1.0 942,273 + -0.4 942,281 - -0.8 942,400 + -0.3 942,641 + -0.1 942,670 - -0.8
Or see this region's nucleotide sequence