Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS00405 and HSERO_RS00410 are separated by 73 nucleotides HSERO_RS00410 and HSERO_RS00415 are separated by 427 nucleotides HSERO_RS00415 and HSERO_RS00420 are separated by 12 nucleotides HSERO_RS00420 and HSERO_RS00425 are separated by 15 nucleotides
HSERO_RS00405: HSERO_RS00405 - Fis family transcriptional regulator, at 95,981 to 97,504
_RS00405
HSERO_RS00410: HSERO_RS00410 - phosphoheptose isomerase, at 97,578 to 97,823
_RS00410
HSERO_RS00415: HSERO_RS00415 - hypothetical protein, at 98,251 to 99,039
_RS00415
HSERO_RS00420: HSERO_RS00420 - nitrogen regulatory protein P-II 1, at 99,052 to 99,390
_RS00420
HSERO_RS00425: HSERO_RS00425 - ammonia channel protein, at 99,406 to 100,938
_RS00425
Position (kb) 98
99
100 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 97.258 kb on + strand, within HSERO_RS00405 at 97.301 kb on + strand, within HSERO_RS00405 at 97.308 kb on + strand, within HSERO_RS00405 at 97.382 kb on - strand at 97.394 kb on - strand at 97.507 kb on + strand at 97.515 kb on - strand at 97.631 kb on - strand, within HSERO_RS00410 at 97.750 kb on + strand, within HSERO_RS00410 at 97.782 kb on - strand, within HSERO_RS00410 at 97.831 kb on - strand at 97.871 kb on - strand at 97.907 kb on - strand at 97.907 kb on - strand at 97.965 kb on + strand at 97.975 kb on - strand at 97.994 kb on - strand at 98.059 kb on - strand at 98.099 kb on - strand at 98.149 kb on + strand at 98.187 kb on - strand at 98.192 kb on - strand at 98.248 kb on + strand at 98.299 kb on + strand at 98.317 kb on - strand at 98.317 kb on - strand at 98.386 kb on + strand, within HSERO_RS00415 at 98.444 kb on - strand, within HSERO_RS00415 at 98.500 kb on + strand, within HSERO_RS00415 at 98.607 kb on + strand, within HSERO_RS00415 at 98.620 kb on - strand, within HSERO_RS00415 at 98.630 kb on - strand, within HSERO_RS00415 at 98.630 kb on - strand, within HSERO_RS00415 at 98.809 kb on - strand, within HSERO_RS00415 at 98.835 kb on + strand, within HSERO_RS00415 at 98.859 kb on + strand, within HSERO_RS00415 at 98.912 kb on + strand, within HSERO_RS00415 at 98.920 kb on - strand, within HSERO_RS00415 at 98.926 kb on - strand at 98.928 kb on + strand, within HSERO_RS00415 at 99.055 kb on + strand at 99.058 kb on - strand at 99.058 kb on - strand at 99.393 kb on - strand at 99.711 kb on + strand, within HSERO_RS00425 at 99.711 kb on + strand, within HSERO_RS00425 at 99.719 kb on - strand, within HSERO_RS00425 at 99.719 kb on - strand, within HSERO_RS00425 at 99.729 kb on - strand, within HSERO_RS00425 at 99.884 kb on - strand, within HSERO_RS00425 at 99.979 kb on - strand, within HSERO_RS00425
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 97,258 + HSERO_RS00405 0.84 -0.4 97,301 + HSERO_RS00405 0.87 -0.4 97,308 + HSERO_RS00405 0.87 +0.2 97,382 - -0.1 97,394 - +0.4 97,507 + +0.8 97,515 - -0.1 97,631 - HSERO_RS00410 0.22 -1.9 97,750 + HSERO_RS00410 0.70 -0.7 97,782 - HSERO_RS00410 0.83 +0.3 97,831 - -0.4 97,871 - -0.3 97,907 - +0.3 97,907 - -0.6 97,965 + -0.7 97,975 - +0.1 97,994 - +0.6 98,059 - +0.3 98,099 - -1.1 98,149 + -1.1 98,187 - -4.5 98,192 - -3.1 98,248 + -2.9 98,299 + -1.5 98,317 - -3.8 98,317 - -3.8 98,386 + HSERO_RS00415 0.17 -3.3 98,444 - HSERO_RS00415 0.24 -2.9 98,500 + HSERO_RS00415 0.32 -1.5 98,607 + HSERO_RS00415 0.45 -2.7 98,620 - HSERO_RS00415 0.47 -3.8 98,630 - HSERO_RS00415 0.48 -2.8 98,630 - HSERO_RS00415 0.48 -2.6 98,809 - HSERO_RS00415 0.71 -3.7 98,835 + HSERO_RS00415 0.74 -2.4 98,859 + HSERO_RS00415 0.77 -2.1 98,912 + HSERO_RS00415 0.84 -0.9 98,920 - HSERO_RS00415 0.85 -2.7 98,926 - -1.3 98,928 + HSERO_RS00415 0.86 -1.1 99,055 + -2.8 99,058 - -0.8 99,058 - -3.4 99,393 - -2.2 99,711 + HSERO_RS00425 0.20 -1.6 99,711 + HSERO_RS00425 0.20 -2.7 99,719 - HSERO_RS00425 0.20 -2.5 99,719 - HSERO_RS00425 0.20 -2.6 99,729 - HSERO_RS00425 0.21 -3.4 99,884 - HSERO_RS00425 0.31 -2.0 99,979 - HSERO_RS00425 0.37 -2.7
Or see this region's nucleotide sequence