Experiment: KB with Nonanoic 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1951 and Psyr_1952 are separated by 35 nucleotides Psyr_1952 and Psyr_1953 overlap by 4 nucleotides Psyr_1953 and Psyr_1954 overlap by 4 nucleotides Psyr_1954 and Psyr_1955 overlap by 4 nucleotides
Psyr_1951: Psyr_1951 - Periplasmic solute binding protein, at 2,235,967 to 2,236,899
_1951
Psyr_1952: Psyr_1952 - hypothetical protein, at 2,236,935 to 2,237,501
_1952
Psyr_1953: Psyr_1953 - conserved hypothetical protein, at 2,237,498 to 2,237,830
_1953
Psyr_1954: Psyr_1954 - conserved hypothetical protein, at 2,237,827 to 2,238,372
_1954
Psyr_1955: Psyr_1955 - Propeptide, PepSY amd peptidase M4:PepSY-associated TM helix, at 2,238,369 to 2,239,592
_1955
Position (kb)
2237
2238
2239 Strain fitness (log2 ratio)
-1
0
1 at 2237.302 kb on + strand, within Psyr_1952 at 2237.303 kb on - strand, within Psyr_1952 at 2237.480 kb on + strand at 2237.565 kb on + strand, within Psyr_1953 at 2237.565 kb on + strand, within Psyr_1953 at 2237.565 kb on + strand, within Psyr_1953 at 2237.566 kb on - strand, within Psyr_1953 at 2237.566 kb on - strand, within Psyr_1953 at 2237.644 kb on - strand, within Psyr_1953 at 2238.057 kb on - strand, within Psyr_1954 at 2238.117 kb on - strand, within Psyr_1954 at 2238.254 kb on + strand, within Psyr_1954 at 2238.255 kb on - strand, within Psyr_1954 at 2238.495 kb on - strand, within Psyr_1955 at 2238.748 kb on + strand, within Psyr_1955 at 2239.175 kb on + strand, within Psyr_1955 at 2239.274 kb on - strand, within Psyr_1955 at 2239.274 kb on - strand, within Psyr_1955 at 2239.327 kb on + strand, within Psyr_1955
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nonanoic 10 mM remove 2,237,302 + Psyr_1952 0.65 -0.3 2,237,303 - Psyr_1952 0.65 +0.4 2,237,480 + +0.2 2,237,565 + Psyr_1953 0.20 -0.2 2,237,565 + Psyr_1953 0.20 -0.7 2,237,565 + Psyr_1953 0.20 -0.2 2,237,566 - Psyr_1953 0.20 -0.9 2,237,566 - Psyr_1953 0.20 +0.1 2,237,644 - Psyr_1953 0.44 -0.0 2,238,057 - Psyr_1954 0.42 -0.9 2,238,117 - Psyr_1954 0.53 -1.0 2,238,254 + Psyr_1954 0.78 -1.3 2,238,255 - Psyr_1954 0.78 -1.4 2,238,495 - Psyr_1955 0.10 +0.1 2,238,748 + Psyr_1955 0.31 -0.1 2,239,175 + Psyr_1955 0.66 -0.1 2,239,274 - Psyr_1955 0.74 -1.7 2,239,274 - Psyr_1955 0.74 -0.9 2,239,327 + Psyr_1955 0.78 +0.8
Or see this region's nucleotide sequence