Experiment: KB with Nonanoic 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0059 and Psyr_0060 overlap by 4 nucleotides Psyr_0060 and Psyr_0061 overlap by 1 nucleotides Psyr_0061 and Psyr_0062 overlap by 4 nucleotides
Psyr_0059: Psyr_0059 - HemY, N-terminal:HemY, N-terminal:HemY, N-terminal, at 58,285 to 59,529
_0059
Psyr_0060: Psyr_0060 - Protein of unknown function DUF513, at 59,526 to 60,701
_0060
Psyr_0061: Psyr_0061 - uroporphyrinogen-III synthase, at 60,701 to 61,474
_0061
Psyr_0062: Psyr_0062 - hydroxymethylbilane synthase, at 61,471 to 62,412
_0062
Position (kb) 59
60
61 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 58.601 kb on + strand, within Psyr_0059 at 58.602 kb on - strand, within Psyr_0059 at 58.602 kb on - strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.626 kb on - strand, within Psyr_0059 at 58.700 kb on + strand, within Psyr_0059 at 59.165 kb on + strand, within Psyr_0059 at 59.165 kb on + strand, within Psyr_0059 at 59.165 kb on + strand, within Psyr_0059 at 59.166 kb on - strand, within Psyr_0059 at 59.166 kb on - strand, within Psyr_0059 at 59.166 kb on - strand, within Psyr_0059 at 59.166 kb on - strand, within Psyr_0059 at 59.166 kb on - strand, within Psyr_0059 at 59.168 kb on + strand, within Psyr_0059 at 59.168 kb on + strand, within Psyr_0059 at 59.168 kb on + strand, within Psyr_0059 at 59.168 kb on + strand, within Psyr_0059 at 59.169 kb on - strand, within Psyr_0059 at 59.169 kb on - strand, within Psyr_0059 at 59.169 kb on - strand, within Psyr_0059 at 59.169 kb on - strand, within Psyr_0059 at 59.169 kb on - strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.433 kb on - strand at 59.447 kb on + strand at 59.447 kb on + strand at 59.448 kb on - strand at 59.448 kb on - strand at 59.506 kb on + strand at 59.507 kb on - strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.587 kb on + strand at 59.588 kb on - strand at 59.749 kb on + strand, within Psyr_0060 at 59.750 kb on - strand, within Psyr_0060 at 59.888 kb on - strand, within Psyr_0060 at 60.004 kb on - strand, within Psyr_0060 at 60.004 kb on - strand, within Psyr_0060 at 60.371 kb on + strand, within Psyr_0060 at 60.537 kb on + strand, within Psyr_0060 at 60.538 kb on - strand, within Psyr_0060 at 60.650 kb on - strand at 60.673 kb on + strand at 60.673 kb on + strand at 60.674 kb on - strand at 60.674 kb on - strand at 60.676 kb on + strand at 60.681 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nonanoic 10 mM remove 58,601 + Psyr_0059 0.25 +0.3 58,602 - Psyr_0059 0.25 +1.7 58,602 - Psyr_0059 0.25 -0.4 58,625 + Psyr_0059 0.27 -0.7 58,626 - Psyr_0059 0.27 +0.2 58,700 + Psyr_0059 0.33 -0.6 59,165 + Psyr_0059 0.71 -1.5 59,165 + Psyr_0059 0.71 -0.1 59,165 + Psyr_0059 0.71 -0.9 59,166 - Psyr_0059 0.71 +0.2 59,166 - Psyr_0059 0.71 -1.7 59,166 - Psyr_0059 0.71 -0.0 59,166 - Psyr_0059 0.71 -0.6 59,166 - Psyr_0059 0.71 -0.7 59,168 + Psyr_0059 0.71 +0.1 59,168 + Psyr_0059 0.71 +0.4 59,168 + Psyr_0059 0.71 -0.9 59,168 + Psyr_0059 0.71 -0.3 59,169 - Psyr_0059 0.71 -0.5 59,169 - Psyr_0059 0.71 +0.5 59,169 - Psyr_0059 0.71 +0.4 59,169 - Psyr_0059 0.71 +0.8 59,169 - Psyr_0059 0.71 +0.3 59,269 + Psyr_0059 0.79 +1.4 59,269 + Psyr_0059 0.79 -1.2 59,269 + Psyr_0059 0.79 -1.9 59,269 + Psyr_0059 0.79 +2.4 59,269 + Psyr_0059 0.79 -0.9 59,269 + Psyr_0059 0.79 -1.6 59,269 + Psyr_0059 0.79 -1.4 59,269 + Psyr_0059 0.79 -0.6 59,270 - Psyr_0059 0.79 +0.0 59,270 - Psyr_0059 0.79 +0.1 59,270 - Psyr_0059 0.79 -2.6 59,270 - Psyr_0059 0.79 -2.4 59,270 - Psyr_0059 0.79 -0.9 59,270 - Psyr_0059 0.79 -1.8 59,270 - Psyr_0059 0.79 -1.3 59,270 - Psyr_0059 0.79 +0.1 59,433 - -0.8 59,447 + -0.3 59,447 + +0.2 59,448 - +0.5 59,448 - +1.0 59,506 + -1.2 59,507 - -3.8 59,516 + -1.6 59,516 + +2.4 59,516 + +0.1 59,516 + -1.5 59,516 + -2.4 59,516 + -0.4 59,516 + -2.3 59,517 - +0.2 59,517 - +2.2 59,517 - -0.6 59,517 - -0.6 59,517 - -1.2 59,517 - -0.0 59,517 - +1.8 59,517 - -1.0 59,517 - -1.4 59,517 - +0.2 59,517 - +0.0 59,517 - +2.4 59,517 - +2.3 59,587 + -0.0 59,588 - +0.1 59,749 + Psyr_0060 0.19 -0.8 59,750 - Psyr_0060 0.19 -0.9 59,888 - Psyr_0060 0.31 +3.0 60,004 - Psyr_0060 0.41 +1.2 60,004 - Psyr_0060 0.41 -1.8 60,371 + Psyr_0060 0.72 -2.3 60,537 + Psyr_0060 0.86 -0.9 60,538 - Psyr_0060 0.86 -1.6 60,650 - -1.5 60,673 + -0.9 60,673 + -1.2 60,674 - +0.8 60,674 - +0.2 60,676 + +4.0 60,681 + -1.3
Or see this region's nucleotide sequence