Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1080

Experiment: Sisomicin 0.0008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1079 and Pf6N2E2_1080 are separated by 11 nucleotidesPf6N2E2_1080 and Pf6N2E2_1081 are separated by 85 nucleotidesPf6N2E2_1081 and Pf6N2E2_1082 overlap by 1 nucleotides Pf6N2E2_1079 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 1,263,441 to 1,264,403 _1079 Pf6N2E2_1080 - hydrolase, alpha/beta fold family, at 1,264,415 to 1,265,446 _1080 Pf6N2E2_1081 - probable membrane protein YPO3302, at 1,265,532 to 1,265,966 _1081 Pf6N2E2_1082 - CopG protein, at 1,265,966 to 1,266,412 _1082 Position (kb) 1264 1265 1266Strain fitness (log2 ratio) -2 -1 0 1 2at 1263.515 kb on - strandat 1263.526 kb on + strandat 1263.544 kb on + strand, within Pf6N2E2_1079at 1263.544 kb on + strand, within Pf6N2E2_1079at 1263.544 kb on + strand, within Pf6N2E2_1079at 1263.545 kb on - strand, within Pf6N2E2_1079at 1263.567 kb on + strand, within Pf6N2E2_1079at 1263.567 kb on + strand, within Pf6N2E2_1079at 1263.567 kb on + strand, within Pf6N2E2_1079at 1263.568 kb on - strand, within Pf6N2E2_1079at 1263.621 kb on + strand, within Pf6N2E2_1079at 1263.621 kb on + strand, within Pf6N2E2_1079at 1263.621 kb on + strand, within Pf6N2E2_1079at 1263.622 kb on - strand, within Pf6N2E2_1079at 1263.835 kb on + strand, within Pf6N2E2_1079at 1263.835 kb on + strand, within Pf6N2E2_1079at 1263.835 kb on + strand, within Pf6N2E2_1079at 1263.835 kb on + strand, within Pf6N2E2_1079at 1263.835 kb on + strand, within Pf6N2E2_1079at 1263.835 kb on + strand, within Pf6N2E2_1079at 1263.835 kb on + strand, within Pf6N2E2_1079at 1263.835 kb on + strand, within Pf6N2E2_1079at 1263.836 kb on - strand, within Pf6N2E2_1079at 1263.836 kb on - strand, within Pf6N2E2_1079at 1263.836 kb on - strand, within Pf6N2E2_1079at 1264.093 kb on + strand, within Pf6N2E2_1079at 1264.093 kb on + strand, within Pf6N2E2_1079at 1264.093 kb on + strand, within Pf6N2E2_1079at 1264.093 kb on + strand, within Pf6N2E2_1079at 1264.094 kb on - strand, within Pf6N2E2_1079at 1264.094 kb on - strand, within Pf6N2E2_1079at 1264.094 kb on - strand, within Pf6N2E2_1079at 1264.094 kb on - strand, within Pf6N2E2_1079at 1264.094 kb on - strand, within Pf6N2E2_1079at 1264.110 kb on + strand, within Pf6N2E2_1079at 1264.110 kb on + strand, within Pf6N2E2_1079at 1264.298 kb on - strand, within Pf6N2E2_1079at 1264.380 kb on + strandat 1264.380 kb on + strandat 1264.389 kb on + strandat 1264.389 kb on + strandat 1264.390 kb on - strandat 1264.390 kb on - strandat 1264.554 kb on - strand, within Pf6N2E2_1080at 1264.560 kb on + strand, within Pf6N2E2_1080at 1264.560 kb on + strand, within Pf6N2E2_1080at 1264.674 kb on + strand, within Pf6N2E2_1080at 1264.675 kb on - strand, within Pf6N2E2_1080at 1264.701 kb on + strand, within Pf6N2E2_1080at 1264.701 kb on + strand, within Pf6N2E2_1080at 1264.701 kb on + strand, within Pf6N2E2_1080at 1264.702 kb on - strand, within Pf6N2E2_1080at 1264.893 kb on - strandat 1265.028 kb on - strand, within Pf6N2E2_1080at 1265.089 kb on - strand, within Pf6N2E2_1080at 1265.102 kb on - strand, within Pf6N2E2_1080at 1265.263 kb on - strand, within Pf6N2E2_1080at 1265.316 kb on + strand, within Pf6N2E2_1080at 1265.317 kb on - strand, within Pf6N2E2_1080at 1265.322 kb on + strand, within Pf6N2E2_1080at 1265.350 kb on - strandat 1265.492 kb on - strandat 1265.710 kb on + strand, within Pf6N2E2_1081at 1265.953 kb on + strandat 1265.953 kb on + strandat 1265.953 kb on + strandat 1265.953 kb on + strandat 1265.953 kb on + strandat 1265.954 kb on - strandat 1265.954 kb on - strandat 1266.043 kb on + strand, within Pf6N2E2_1082at 1266.043 kb on + strand, within Pf6N2E2_1082at 1266.044 kb on - strand, within Pf6N2E2_1082

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.0008 mg/ml
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1,263,515 - +0.2
1,263,526 + -0.9
1,263,544 + Pf6N2E2_1079 0.11 +1.5
1,263,544 + Pf6N2E2_1079 0.11 +0.5
1,263,544 + Pf6N2E2_1079 0.11 +1.3
1,263,545 - Pf6N2E2_1079 0.11 +0.8
1,263,567 + Pf6N2E2_1079 0.13 +0.3
1,263,567 + Pf6N2E2_1079 0.13 -0.1
1,263,567 + Pf6N2E2_1079 0.13 -1.1
1,263,568 - Pf6N2E2_1079 0.13 -2.1
1,263,621 + Pf6N2E2_1079 0.19 +0.4
1,263,621 + Pf6N2E2_1079 0.19 +1.1
1,263,621 + Pf6N2E2_1079 0.19 -0.8
1,263,622 - Pf6N2E2_1079 0.19 +0.7
1,263,835 + Pf6N2E2_1079 0.41 -0.2
1,263,835 + Pf6N2E2_1079 0.41 +0.3
1,263,835 + Pf6N2E2_1079 0.41 +0.5
1,263,835 + Pf6N2E2_1079 0.41 -0.9
1,263,835 + Pf6N2E2_1079 0.41 +1.5
1,263,835 + Pf6N2E2_1079 0.41 +1.9
1,263,835 + Pf6N2E2_1079 0.41 +1.0
1,263,835 + Pf6N2E2_1079 0.41 -0.1
1,263,836 - Pf6N2E2_1079 0.41 -0.8
1,263,836 - Pf6N2E2_1079 0.41 +0.3
1,263,836 - Pf6N2E2_1079 0.41 -0.1
1,264,093 + Pf6N2E2_1079 0.68 +1.1
1,264,093 + Pf6N2E2_1079 0.68 +0.7
1,264,093 + Pf6N2E2_1079 0.68 -0.0
1,264,093 + Pf6N2E2_1079 0.68 +0.4
1,264,094 - Pf6N2E2_1079 0.68 -0.7
1,264,094 - Pf6N2E2_1079 0.68 +0.9
1,264,094 - Pf6N2E2_1079 0.68 -0.4
1,264,094 - Pf6N2E2_1079 0.68 -0.6
1,264,094 - Pf6N2E2_1079 0.68 +0.3
1,264,110 + Pf6N2E2_1079 0.69 -0.6
1,264,110 + Pf6N2E2_1079 0.69 +0.1
1,264,298 - Pf6N2E2_1079 0.89 -0.4
1,264,380 + +0.2
1,264,380 + -0.7
1,264,389 + -1.1
1,264,389 + -0.8
1,264,390 - -1.1
1,264,390 - -0.1
1,264,554 - Pf6N2E2_1080 0.13 -0.5
1,264,560 + Pf6N2E2_1080 0.14 +0.6
1,264,560 + Pf6N2E2_1080 0.14 +0.6
1,264,674 + Pf6N2E2_1080 0.25 +0.9
1,264,675 - Pf6N2E2_1080 0.25 +1.5
1,264,701 + Pf6N2E2_1080 0.28 +1.5
1,264,701 + Pf6N2E2_1080 0.28 +0.7
1,264,701 + Pf6N2E2_1080 0.28 +0.2
1,264,702 - Pf6N2E2_1080 0.28 +0.9
1,264,893 - +0.2
1,265,028 - Pf6N2E2_1080 0.59 +1.5
1,265,089 - Pf6N2E2_1080 0.65 +0.7
1,265,102 - Pf6N2E2_1080 0.67 +1.1
1,265,263 - Pf6N2E2_1080 0.82 +0.6
1,265,316 + Pf6N2E2_1080 0.87 +0.7
1,265,317 - Pf6N2E2_1080 0.87 +0.2
1,265,322 + Pf6N2E2_1080 0.88 -0.1
1,265,350 - +1.5
1,265,492 - -1.2
1,265,710 + Pf6N2E2_1081 0.41 +0.4
1,265,953 + -0.5
1,265,953 + -0.7
1,265,953 + -0.4
1,265,953 + -0.1
1,265,953 + -0.8
1,265,954 - +0.1
1,265,954 - -0.2
1,266,043 + Pf6N2E2_1082 0.17 -1.0
1,266,043 + Pf6N2E2_1082 0.17 -0.7
1,266,044 - Pf6N2E2_1082 0.17 -0.4

Or see this region's nucleotide sequence