Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2802

Experiment: Lomefloxacin 0.0002 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2801 and Pf6N2E2_2802 are separated by 140 nucleotidesPf6N2E2_2802 and Pf6N2E2_2803 are separated by 140 nucleotides Pf6N2E2_2801 - Bicyclomycin resistance protein, at 3,287,048 to 3,288,211 _2801 Pf6N2E2_2802 - Alcohol dehydrogenase (EC 1.1.1.1), at 3,288,352 to 3,289,500 _2802 Pf6N2E2_2803 - FIG00953317: hypothetical protein, at 3,289,641 to 3,290,660 _2803 Position (kb) 3288 3289 3290Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3287.486 kb on + strand, within Pf6N2E2_2801at 3287.486 kb on + strand, within Pf6N2E2_2801at 3287.487 kb on - strand, within Pf6N2E2_2801at 3287.636 kb on + strand, within Pf6N2E2_2801at 3287.637 kb on - strand, within Pf6N2E2_2801at 3287.637 kb on - strand, within Pf6N2E2_2801at 3287.771 kb on + strand, within Pf6N2E2_2801at 3287.771 kb on + strand, within Pf6N2E2_2801at 3287.772 kb on - strand, within Pf6N2E2_2801at 3287.804 kb on + strand, within Pf6N2E2_2801at 3287.804 kb on + strand, within Pf6N2E2_2801at 3287.804 kb on + strand, within Pf6N2E2_2801at 3287.804 kb on + strand, within Pf6N2E2_2801at 3287.805 kb on - strand, within Pf6N2E2_2801at 3287.838 kb on + strand, within Pf6N2E2_2801at 3287.839 kb on - strand, within Pf6N2E2_2801at 3287.993 kb on + strand, within Pf6N2E2_2801at 3287.993 kb on + strand, within Pf6N2E2_2801at 3287.993 kb on + strand, within Pf6N2E2_2801at 3287.994 kb on - strand, within Pf6N2E2_2801at 3288.045 kb on - strand, within Pf6N2E2_2801at 3288.219 kb on - strandat 3288.344 kb on + strandat 3288.344 kb on + strandat 3288.344 kb on + strandat 3288.345 kb on - strandat 3288.345 kb on - strandat 3288.353 kb on + strandat 3288.353 kb on + strandat 3288.353 kb on + strandat 3288.353 kb on + strandat 3288.354 kb on - strandat 3288.455 kb on - strandat 3288.469 kb on + strand, within Pf6N2E2_2802at 3288.469 kb on + strand, within Pf6N2E2_2802at 3288.470 kb on - strand, within Pf6N2E2_2802at 3288.574 kb on - strand, within Pf6N2E2_2802at 3288.686 kb on + strand, within Pf6N2E2_2802at 3288.686 kb on + strand, within Pf6N2E2_2802at 3288.686 kb on + strand, within Pf6N2E2_2802at 3288.686 kb on + strand, within Pf6N2E2_2802at 3288.686 kb on + strand, within Pf6N2E2_2802at 3288.687 kb on - strand, within Pf6N2E2_2802at 3288.687 kb on - strand, within Pf6N2E2_2802at 3288.687 kb on - strand, within Pf6N2E2_2802at 3288.771 kb on - strand, within Pf6N2E2_2802at 3288.771 kb on - strand, within Pf6N2E2_2802at 3288.896 kb on + strand, within Pf6N2E2_2802at 3288.896 kb on + strand, within Pf6N2E2_2802at 3288.896 kb on + strand, within Pf6N2E2_2802at 3288.896 kb on + strand, within Pf6N2E2_2802at 3288.896 kb on + strand, within Pf6N2E2_2802at 3288.896 kb on + strand, within Pf6N2E2_2802at 3288.896 kb on + strand, within Pf6N2E2_2802at 3288.896 kb on + strand, within Pf6N2E2_2802at 3288.897 kb on - strand, within Pf6N2E2_2802at 3288.897 kb on - strand, within Pf6N2E2_2802at 3289.145 kb on + strand, within Pf6N2E2_2802at 3289.146 kb on - strand, within Pf6N2E2_2802at 3289.146 kb on - strand, within Pf6N2E2_2802at 3289.146 kb on - strand, within Pf6N2E2_2802at 3289.554 kb on + strandat 3289.659 kb on + strandat 3289.659 kb on + strandat 3289.944 kb on + strand, within Pf6N2E2_2803at 3289.945 kb on - strand, within Pf6N2E2_2803at 3290.074 kb on - strand, within Pf6N2E2_2803at 3290.074 kb on - strand, within Pf6N2E2_2803at 3290.074 kb on - strand, within Pf6N2E2_2803at 3290.193 kb on + strand, within Pf6N2E2_2803at 3290.193 kb on + strand, within Pf6N2E2_2803at 3290.379 kb on + strand, within Pf6N2E2_2803at 3290.380 kb on - strand, within Pf6N2E2_2803at 3290.380 kb on - strand, within Pf6N2E2_2803at 3290.380 kb on - strand, within Pf6N2E2_2803at 3290.380 kb on - strand, within Pf6N2E2_2803at 3290.394 kb on + strand, within Pf6N2E2_2803

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Per-strain Table

Position Strand Gene LocusTag Fraction Lomefloxacin 0.0002 mM
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3,287,486 + Pf6N2E2_2801 0.38 +1.1
3,287,486 + Pf6N2E2_2801 0.38 +0.0
3,287,487 - Pf6N2E2_2801 0.38 -0.9
3,287,636 + Pf6N2E2_2801 0.51 -0.1
3,287,637 - Pf6N2E2_2801 0.51 +0.7
3,287,637 - Pf6N2E2_2801 0.51 +1.3
3,287,771 + Pf6N2E2_2801 0.62 -0.7
3,287,771 + Pf6N2E2_2801 0.62 -1.0
3,287,772 - Pf6N2E2_2801 0.62 -0.2
3,287,804 + Pf6N2E2_2801 0.65 -0.4
3,287,804 + Pf6N2E2_2801 0.65 +0.2
3,287,804 + Pf6N2E2_2801 0.65 -0.6
3,287,804 + Pf6N2E2_2801 0.65 -0.0
3,287,805 - Pf6N2E2_2801 0.65 -1.8
3,287,838 + Pf6N2E2_2801 0.68 -0.3
3,287,839 - Pf6N2E2_2801 0.68 -0.9
3,287,993 + Pf6N2E2_2801 0.81 +0.3
3,287,993 + Pf6N2E2_2801 0.81 -0.8
3,287,993 + Pf6N2E2_2801 0.81 -1.2
3,287,994 - Pf6N2E2_2801 0.81 +0.1
3,288,045 - Pf6N2E2_2801 0.86 +0.3
3,288,219 - -2.7
3,288,344 + +0.1
3,288,344 + +0.3
3,288,344 + -1.0
3,288,345 - -0.3
3,288,345 - -1.2
3,288,353 + +1.9
3,288,353 + -1.0
3,288,353 + -0.1
3,288,353 + +0.3
3,288,354 - +0.6
3,288,455 - -1.2
3,288,469 + Pf6N2E2_2802 0.10 -0.3
3,288,469 + Pf6N2E2_2802 0.10 -0.9
3,288,470 - Pf6N2E2_2802 0.10 +0.0
3,288,574 - Pf6N2E2_2802 0.19 +1.5
3,288,686 + Pf6N2E2_2802 0.29 -0.5
3,288,686 + Pf6N2E2_2802 0.29 +0.1
3,288,686 + Pf6N2E2_2802 0.29 +0.4
3,288,686 + Pf6N2E2_2802 0.29 +0.5
3,288,686 + Pf6N2E2_2802 0.29 -1.1
3,288,687 - Pf6N2E2_2802 0.29 +1.0
3,288,687 - Pf6N2E2_2802 0.29 -0.5
3,288,687 - Pf6N2E2_2802 0.29 -0.6
3,288,771 - Pf6N2E2_2802 0.36 -1.4
3,288,771 - Pf6N2E2_2802 0.36 -0.7
3,288,896 + Pf6N2E2_2802 0.47 -0.4
3,288,896 + Pf6N2E2_2802 0.47 -0.5
3,288,896 + Pf6N2E2_2802 0.47 +0.4
3,288,896 + Pf6N2E2_2802 0.47 +0.2
3,288,896 + Pf6N2E2_2802 0.47 +2.1
3,288,896 + Pf6N2E2_2802 0.47 -1.4
3,288,896 + Pf6N2E2_2802 0.47 -1.1
3,288,896 + Pf6N2E2_2802 0.47 +0.8
3,288,897 - Pf6N2E2_2802 0.47 -0.7
3,288,897 - Pf6N2E2_2802 0.47 +0.1
3,289,145 + Pf6N2E2_2802 0.69 +0.4
3,289,146 - Pf6N2E2_2802 0.69 -0.2
3,289,146 - Pf6N2E2_2802 0.69 -0.2
3,289,146 - Pf6N2E2_2802 0.69 +0.5
3,289,554 + +0.3
3,289,659 + -1.4
3,289,659 + -1.3
3,289,944 + Pf6N2E2_2803 0.30 +0.7
3,289,945 - Pf6N2E2_2803 0.30 -1.0
3,290,074 - Pf6N2E2_2803 0.42 -1.1
3,290,074 - Pf6N2E2_2803 0.42 -0.1
3,290,074 - Pf6N2E2_2803 0.42 -0.3
3,290,193 + Pf6N2E2_2803 0.54 +0.6
3,290,193 + Pf6N2E2_2803 0.54 -0.9
3,290,379 + Pf6N2E2_2803 0.72 -2.9
3,290,380 - Pf6N2E2_2803 0.72 -0.5
3,290,380 - Pf6N2E2_2803 0.72 +0.1
3,290,380 - Pf6N2E2_2803 0.72 -0.2
3,290,380 - Pf6N2E2_2803 0.72 +0.1
3,290,394 + Pf6N2E2_2803 0.74 +0.1

Or see this region's nucleotide sequence