Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_776

Experiment: Fusidic 0.4 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_773 and Pf6N2E2_774 overlap by 4 nucleotidesPf6N2E2_774 and Pf6N2E2_775 are separated by 4 nucleotidesPf6N2E2_775 and Pf6N2E2_776 overlap by 4 nucleotidesPf6N2E2_776 and Pf6N2E2_777 are separated by 128 nucleotidesPf6N2E2_777 and Pf6N2E2_778 are separated by 76 nucleotides Pf6N2E2_773 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 884,233 to 885,063 _773 Pf6N2E2_774 - Cold-shock DNA-binding domain, at 885,060 to 885,479 _774 Pf6N2E2_775 - FIG00955061: hypothetical protein, at 885,484 to 885,834 _775 Pf6N2E2_776 - Transcriptional regulator, LysR family, at 885,831 to 886,739 _776 Pf6N2E2_777 - FMN-dependent NADH-azoreductase, at 886,868 to 887,476 _777 Pf6N2E2_778 - Probable lipoprotein signal peptide, at 887,553 to 888,698 _778 Position (kb) 885 886 887Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6at 884.837 kb on - strand, within Pf6N2E2_773at 884.846 kb on - strand, within Pf6N2E2_773at 884.846 kb on - strand, within Pf6N2E2_773at 884.851 kb on + strand, within Pf6N2E2_773at 884.968 kb on + strand, within Pf6N2E2_773at 884.968 kb on + strand, within Pf6N2E2_773at 884.968 kb on + strand, within Pf6N2E2_773at 884.969 kb on - strand, within Pf6N2E2_773at 884.969 kb on - strand, within Pf6N2E2_773at 884.969 kb on - strand, within Pf6N2E2_773at 884.969 kb on - strand, within Pf6N2E2_773at 884.969 kb on - strand, within Pf6N2E2_773at 884.969 kb on - strand, within Pf6N2E2_773at 885.469 kb on - strandat 885.485 kb on + strandat 885.486 kb on - strandat 885.486 kb on - strandat 885.624 kb on - strand, within Pf6N2E2_775at 885.770 kb on + strand, within Pf6N2E2_775at 885.771 kb on - strand, within Pf6N2E2_775at 885.771 kb on - strand, within Pf6N2E2_775at 885.771 kb on - strand, within Pf6N2E2_775at 885.771 kb on - strand, within Pf6N2E2_775at 885.771 kb on - strand, within Pf6N2E2_775at 885.834 kb on + strandat 885.842 kb on + strandat 885.843 kb on - strandat 885.843 kb on - strandat 885.843 kb on - strandat 886.006 kb on + strand, within Pf6N2E2_776at 886.006 kb on + strand, within Pf6N2E2_776at 886.006 kb on + strand, within Pf6N2E2_776at 886.006 kb on + strand, within Pf6N2E2_776at 886.006 kb on + strand, within Pf6N2E2_776at 886.006 kb on + strandat 886.006 kb on + strand, within Pf6N2E2_776at 886.007 kb on - strand, within Pf6N2E2_776at 886.007 kb on - strand, within Pf6N2E2_776at 886.007 kb on - strand, within Pf6N2E2_776at 886.010 kb on + strand, within Pf6N2E2_776at 886.010 kb on + strand, within Pf6N2E2_776at 886.011 kb on - strand, within Pf6N2E2_776at 886.020 kb on + strand, within Pf6N2E2_776at 886.367 kb on + strand, within Pf6N2E2_776at 886.367 kb on + strand, within Pf6N2E2_776at 886.388 kb on - strand, within Pf6N2E2_776at 886.410 kb on + strand, within Pf6N2E2_776at 886.546 kb on + strand, within Pf6N2E2_776at 886.546 kb on + strand, within Pf6N2E2_776at 886.714 kb on + strandat 886.714 kb on + strandat 886.714 kb on + strandat 886.717 kb on + strandat 886.717 kb on + strandat 886.718 kb on - strandat 886.718 kb on - strandat 886.773 kb on + strandat 886.774 kb on - strandat 886.811 kb on - strandat 886.814 kb on + strandat 886.814 kb on + strandat 886.868 kb on - strandat 886.876 kb on - strandat 886.978 kb on + strand, within Pf6N2E2_777at 886.983 kb on - strand, within Pf6N2E2_777at 886.983 kb on - strand, within Pf6N2E2_777at 887.045 kb on - strand, within Pf6N2E2_777at 887.092 kb on + strand, within Pf6N2E2_777at 887.153 kb on + strand, within Pf6N2E2_777at 887.154 kb on - strand, within Pf6N2E2_777at 887.333 kb on + strand, within Pf6N2E2_777at 887.333 kb on + strand, within Pf6N2E2_777at 887.333 kb on + strand, within Pf6N2E2_777at 887.334 kb on - strand, within Pf6N2E2_777at 887.359 kb on - strand, within Pf6N2E2_777at 887.423 kb on - strandat 887.486 kb on - strandat 887.486 kb on - strandat 887.516 kb on + strandat 887.516 kb on + strandat 887.516 kb on + strandat 887.517 kb on - strandat 887.527 kb on - strandat 887.565 kb on - strandat 887.565 kb on - strandat 887.565 kb on - strandat 887.599 kb on - strandat 887.666 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml
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884,837 - Pf6N2E2_773 0.73 +3.7
884,846 - Pf6N2E2_773 0.74 +3.9
884,846 - Pf6N2E2_773 0.74 +4.6
884,851 + Pf6N2E2_773 0.74 +3.7
884,968 + Pf6N2E2_773 0.88 +3.4
884,968 + Pf6N2E2_773 0.88 +3.0
884,968 + Pf6N2E2_773 0.88 +3.7
884,969 - Pf6N2E2_773 0.89 +4.0
884,969 - Pf6N2E2_773 0.89 +5.3
884,969 - Pf6N2E2_773 0.89 +5.8
884,969 - Pf6N2E2_773 0.89 +4.4
884,969 - Pf6N2E2_773 0.89 +4.5
884,969 - Pf6N2E2_773 0.89 +4.4
885,469 - +1.3
885,485 + -1.8
885,486 - +0.5
885,486 - +1.0
885,624 - Pf6N2E2_775 0.40 +0.7
885,770 + Pf6N2E2_775 0.81 -0.0
885,771 - Pf6N2E2_775 0.82 +0.6
885,771 - Pf6N2E2_775 0.82 +0.4
885,771 - Pf6N2E2_775 0.82 +0.4
885,771 - Pf6N2E2_775 0.82 +0.2
885,771 - Pf6N2E2_775 0.82 +0.0
885,834 + -1.9
885,842 + -2.4
885,843 - +0.4
885,843 - -1.7
885,843 - +0.6
886,006 + Pf6N2E2_776 0.19 +0.4
886,006 + Pf6N2E2_776 0.19 -1.4
886,006 + Pf6N2E2_776 0.19 -0.4
886,006 + Pf6N2E2_776 0.19 +0.2
886,006 + Pf6N2E2_776 0.19 -0.7
886,006 + +1.2
886,006 + Pf6N2E2_776 0.19 -0.5
886,007 - Pf6N2E2_776 0.19 +1.5
886,007 - Pf6N2E2_776 0.19 +2.1
886,007 - Pf6N2E2_776 0.19 +2.0
886,010 + Pf6N2E2_776 0.20 +0.7
886,010 + Pf6N2E2_776 0.20 +0.8
886,011 - Pf6N2E2_776 0.20 +0.9
886,020 + Pf6N2E2_776 0.21 -0.0
886,367 + Pf6N2E2_776 0.59 +1.0
886,367 + Pf6N2E2_776 0.59 +0.4
886,388 - Pf6N2E2_776 0.61 +1.8
886,410 + Pf6N2E2_776 0.64 -0.6
886,546 + Pf6N2E2_776 0.79 +0.8
886,546 + Pf6N2E2_776 0.79 -2.3
886,714 + -0.1
886,714 + +0.1
886,714 + -0.2
886,717 + -0.2
886,717 + -0.7
886,718 - +0.2
886,718 - -0.5
886,773 + -1.3
886,774 - +1.1
886,811 - +1.2
886,814 + +0.2
886,814 + +1.8
886,868 - +1.0
886,876 - +0.8
886,978 + Pf6N2E2_777 0.18 +0.0
886,983 - Pf6N2E2_777 0.19 +0.7
886,983 - Pf6N2E2_777 0.19 +0.1
887,045 - Pf6N2E2_777 0.29 -0.3
887,092 + Pf6N2E2_777 0.37 +0.4
887,153 + Pf6N2E2_777 0.47 -1.2
887,154 - Pf6N2E2_777 0.47 +1.9
887,333 + Pf6N2E2_777 0.76 +1.3
887,333 + Pf6N2E2_777 0.76 +0.2
887,333 + Pf6N2E2_777 0.76 -0.3
887,334 - Pf6N2E2_777 0.77 +0.2
887,359 - Pf6N2E2_777 0.81 +3.6
887,423 - -0.9
887,486 - +1.7
887,486 - +0.2
887,516 + +0.6
887,516 + +0.7
887,516 + -0.9
887,517 - -0.5
887,527 - +0.3
887,565 - +0.8
887,565 - -0.4
887,565 - +1.4
887,599 - +0.5
887,666 - +0.9

Or see this region's nucleotide sequence