Experiment: Fusidic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_536 and Pf6N2E2_537 overlap by 4 nucleotides Pf6N2E2_537 and Pf6N2E2_538 overlap by 1 nucleotides
Pf6N2E2_536 - Na(+) H(+) antiporter subunit D, at 602,844 to 604,529
_536
Pf6N2E2_537 - Na(+) H(+) antiporter subunit C, at 604,526 to 604,870
_537
Pf6N2E2_538 - Na(+) H(+) antiporter subunit A; Na(+) H(+) antiporter subunit B, at 604,870 to 607,794
_538
Position (kb) 604
605 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 603.688 kb on + strand, within Pf6N2E2_536 at 603.688 kb on + strand, within Pf6N2E2_536 at 603.689 kb on - strand, within Pf6N2E2_536 at 603.748 kb on + strand, within Pf6N2E2_536 at 603.808 kb on + strand, within Pf6N2E2_536 at 603.808 kb on + strand, within Pf6N2E2_536 at 603.808 kb on + strand, within Pf6N2E2_536 at 603.970 kb on + strand, within Pf6N2E2_536 at 603.970 kb on + strand, within Pf6N2E2_536 at 603.971 kb on - strand, within Pf6N2E2_536 at 603.971 kb on - strand, within Pf6N2E2_536 at 603.971 kb on - strand, within Pf6N2E2_536 at 603.971 kb on - strand, within Pf6N2E2_536 at 604.027 kb on + strand, within Pf6N2E2_536 at 604.027 kb on + strand, within Pf6N2E2_536 at 604.028 kb on - strand, within Pf6N2E2_536 at 604.028 kb on - strand, within Pf6N2E2_536 at 604.028 kb on - strand, within Pf6N2E2_536 at 604.028 kb on - strand, within Pf6N2E2_536 at 604.098 kb on + strand, within Pf6N2E2_536 at 604.099 kb on - strand, within Pf6N2E2_536 at 604.099 kb on - strand, within Pf6N2E2_536 at 604.150 kb on + strand, within Pf6N2E2_536 at 604.330 kb on + strand, within Pf6N2E2_536 at 604.372 kb on + strand at 604.372 kb on + strand at 604.373 kb on - strand at 604.668 kb on + strand, within Pf6N2E2_537 at 604.668 kb on + strand, within Pf6N2E2_537 at 604.668 kb on + strand, within Pf6N2E2_537 at 604.668 kb on + strand, within Pf6N2E2_537 at 604.669 kb on - strand, within Pf6N2E2_537 at 604.669 kb on - strand, within Pf6N2E2_537 at 604.761 kb on + strand, within Pf6N2E2_537 at 604.769 kb on + strand, within Pf6N2E2_537 at 604.769 kb on + strand, within Pf6N2E2_537 at 604.770 kb on - strand, within Pf6N2E2_537 at 604.838 kb on + strand at 604.839 kb on - strand at 604.923 kb on + strand at 604.991 kb on + strand at 605.201 kb on + strand, within Pf6N2E2_538 at 605.201 kb on + strand, within Pf6N2E2_538 at 605.201 kb on + strand, within Pf6N2E2_538 at 605.201 kb on + strand, within Pf6N2E2_538 at 605.201 kb on + strand, within Pf6N2E2_538 at 605.201 kb on + strand, within Pf6N2E2_538 at 605.201 kb on + strand, within Pf6N2E2_538 at 605.202 kb on - strand, within Pf6N2E2_538 at 605.202 kb on - strand, within Pf6N2E2_538 at 605.202 kb on - strand, within Pf6N2E2_538 at 605.282 kb on + strand, within Pf6N2E2_538 at 605.282 kb on + strand, within Pf6N2E2_538 at 605.283 kb on - strand, within Pf6N2E2_538 at 605.335 kb on + strand, within Pf6N2E2_538 at 605.597 kb on + strand, within Pf6N2E2_538 at 605.597 kb on + strand, within Pf6N2E2_538 at 605.598 kb on - strand, within Pf6N2E2_538 at 605.598 kb on - strand, within Pf6N2E2_538 at 605.643 kb on - strand, within Pf6N2E2_538
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml remove 603,688 + Pf6N2E2_536 0.50 -0.0 603,688 + Pf6N2E2_536 0.50 +0.4 603,689 - Pf6N2E2_536 0.50 +1.3 603,748 + Pf6N2E2_536 0.54 -0.1 603,808 + Pf6N2E2_536 0.57 +0.4 603,808 + Pf6N2E2_536 0.57 -0.1 603,808 + Pf6N2E2_536 0.57 +0.6 603,970 + Pf6N2E2_536 0.67 -1.4 603,970 + Pf6N2E2_536 0.67 -1.2 603,971 - Pf6N2E2_536 0.67 +1.0 603,971 - Pf6N2E2_536 0.67 -0.8 603,971 - Pf6N2E2_536 0.67 -0.9 603,971 - Pf6N2E2_536 0.67 +1.5 604,027 + Pf6N2E2_536 0.70 -0.3 604,027 + Pf6N2E2_536 0.70 +1.6 604,028 - Pf6N2E2_536 0.70 +0.6 604,028 - Pf6N2E2_536 0.70 -0.7 604,028 - Pf6N2E2_536 0.70 -0.2 604,028 - Pf6N2E2_536 0.70 +1.8 604,098 + Pf6N2E2_536 0.74 -1.5 604,099 - Pf6N2E2_536 0.74 -0.1 604,099 - Pf6N2E2_536 0.74 -0.2 604,150 + Pf6N2E2_536 0.77 +0.1 604,330 + Pf6N2E2_536 0.88 -0.4 604,372 + +1.2 604,372 + +0.2 604,373 - -1.2 604,668 + Pf6N2E2_537 0.41 +1.1 604,668 + Pf6N2E2_537 0.41 +0.5 604,668 + Pf6N2E2_537 0.41 -0.9 604,668 + Pf6N2E2_537 0.41 +5.4 604,669 - Pf6N2E2_537 0.41 -1.8 604,669 - Pf6N2E2_537 0.41 -0.4 604,761 + Pf6N2E2_537 0.68 +0.5 604,769 + Pf6N2E2_537 0.70 -0.5 604,769 + Pf6N2E2_537 0.70 -0.5 604,770 - Pf6N2E2_537 0.71 -0.2 604,838 + -1.1 604,839 - +1.0 604,923 + +1.4 604,991 + -0.1 605,201 + Pf6N2E2_538 0.11 -0.6 605,201 + Pf6N2E2_538 0.11 +0.1 605,201 + Pf6N2E2_538 0.11 -0.6 605,201 + Pf6N2E2_538 0.11 +0.2 605,201 + Pf6N2E2_538 0.11 +1.3 605,201 + Pf6N2E2_538 0.11 +0.2 605,201 + Pf6N2E2_538 0.11 +0.1 605,202 - Pf6N2E2_538 0.11 +0.6 605,202 - Pf6N2E2_538 0.11 -2.8 605,202 - Pf6N2E2_538 0.11 +1.0 605,282 + Pf6N2E2_538 0.14 -1.6 605,282 + Pf6N2E2_538 0.14 +0.0 605,283 - Pf6N2E2_538 0.14 +0.4 605,335 + Pf6N2E2_538 0.16 -0.1 605,597 + Pf6N2E2_538 0.25 +0.4 605,597 + Pf6N2E2_538 0.25 +0.6 605,598 - Pf6N2E2_538 0.25 +1.1 605,598 - Pf6N2E2_538 0.25 +0.8 605,643 - Pf6N2E2_538 0.26 -0.1
Or see this region's nucleotide sequence