Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26275 and BWI76_RS26280 are separated by 121 nucleotides BWI76_RS26280 and BWI76_RS26285 are separated by 23 nucleotides BWI76_RS26285 and BWI76_RS26290 overlap by 4 nucleotides
BWI76_RS26275: BWI76_RS26275 - gamma-glutamyltransferase, at 5,438,258 to 5,440,000
_RS26275
BWI76_RS26280: BWI76_RS26280 - hypothetical protein, at 5,440,122 to 5,440,442
_RS26280
BWI76_RS26285: BWI76_RS26285 - glycerophosphodiester phosphodiesterase, at 5,440,466 to 5,441,206
_RS26285
BWI76_RS26290: BWI76_RS26290 - glycerol-3-phosphate ABC transporter ATP-binding protein, at 5,441,203 to 5,442,273
_RS26290
Position (kb) 5440
5441 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5439.138 kb on - strand, within BWI76_RS26275 at 5439.140 kb on + strand, within BWI76_RS26275 at 5439.140 kb on + strand, within BWI76_RS26275 at 5439.148 kb on - strand, within BWI76_RS26275 at 5439.148 kb on - strand, within BWI76_RS26275 at 5439.212 kb on + strand, within BWI76_RS26275 at 5439.231 kb on + strand, within BWI76_RS26275 at 5439.267 kb on + strand, within BWI76_RS26275 at 5439.417 kb on - strand, within BWI76_RS26275 at 5439.503 kb on + strand, within BWI76_RS26275 at 5439.540 kb on - strand, within BWI76_RS26275 at 5439.572 kb on - strand, within BWI76_RS26275 at 5439.678 kb on + strand, within BWI76_RS26275 at 5439.686 kb on - strand, within BWI76_RS26275 at 5439.742 kb on + strand, within BWI76_RS26275 at 5439.854 kb on + strand at 5439.897 kb on + strand at 5439.897 kb on + strand at 5439.905 kb on - strand at 5439.942 kb on - strand at 5439.963 kb on + strand at 5440.096 kb on - strand at 5440.112 kb on + strand at 5440.115 kb on - strand at 5440.178 kb on + strand, within BWI76_RS26280 at 5440.197 kb on - strand, within BWI76_RS26280 at 5440.218 kb on - strand, within BWI76_RS26280 at 5440.293 kb on + strand, within BWI76_RS26280 at 5440.293 kb on + strand, within BWI76_RS26280 at 5440.601 kb on - strand, within BWI76_RS26285 at 5440.635 kb on - strand, within BWI76_RS26285 at 5440.635 kb on - strand, within BWI76_RS26285 at 5440.698 kb on + strand, within BWI76_RS26285 at 5440.943 kb on - strand, within BWI76_RS26285 at 5441.105 kb on + strand, within BWI76_RS26285 at 5441.113 kb on - strand, within BWI76_RS26285
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 5,439,138 - BWI76_RS26275 0.50 +0.9 5,439,140 + BWI76_RS26275 0.51 +0.6 5,439,140 + BWI76_RS26275 0.51 -0.6 5,439,148 - BWI76_RS26275 0.51 -2.0 5,439,148 - BWI76_RS26275 0.51 +0.3 5,439,212 + BWI76_RS26275 0.55 -1.8 5,439,231 + BWI76_RS26275 0.56 +0.1 5,439,267 + BWI76_RS26275 0.58 +0.2 5,439,417 - BWI76_RS26275 0.66 +0.3 5,439,503 + BWI76_RS26275 0.71 -1.5 5,439,540 - BWI76_RS26275 0.74 +2.4 5,439,572 - BWI76_RS26275 0.75 -0.9 5,439,678 + BWI76_RS26275 0.81 -0.2 5,439,686 - BWI76_RS26275 0.82 +0.0 5,439,742 + BWI76_RS26275 0.85 -0.2 5,439,854 + -0.2 5,439,897 + -0.3 5,439,897 + -0.5 5,439,905 - +0.1 5,439,942 - -0.9 5,439,963 + -1.3 5,440,096 - +1.2 5,440,112 + +0.5 5,440,115 - -0.2 5,440,178 + BWI76_RS26280 0.17 -0.9 5,440,197 - BWI76_RS26280 0.23 +0.0 5,440,218 - BWI76_RS26280 0.30 -0.8 5,440,293 + BWI76_RS26280 0.53 -0.9 5,440,293 + BWI76_RS26280 0.53 -0.7 5,440,601 - BWI76_RS26285 0.18 -0.5 5,440,635 - BWI76_RS26285 0.23 -0.4 5,440,635 - BWI76_RS26285 0.23 -0.5 5,440,698 + BWI76_RS26285 0.31 -0.1 5,440,943 - BWI76_RS26285 0.64 +0.9 5,441,105 + BWI76_RS26285 0.86 -1.7 5,441,113 - BWI76_RS26285 0.87 +0.2
Or see this region's nucleotide sequence