Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS07250

Experiment: Fusidic 1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS07245 and BWI76_RS07250 are separated by 44 nucleotidesBWI76_RS07250 and BWI76_RS07255 overlap by 4 nucleotidesBWI76_RS07255 and BWI76_RS07260 are separated by 17 nucleotides BWI76_RS07245: BWI76_RS07245 - ABC transporter, at 1,524,944 to 1,525,975 _RS07245 BWI76_RS07250: BWI76_RS07250 - Zn-dependent hydrolase, at 1,526,020 to 1,527,261 _RS07250 BWI76_RS07255: BWI76_RS07255 - hypothetical protein, at 1,527,258 to 1,527,740 _RS07255 BWI76_RS07260: BWI76_RS07260 - branched chain amino acid ABC transporter substrate-binding protein, at 1,527,758 to 1,528,915 _RS07260 Position (kb) 1526 1527 1528Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1525.065 kb on + strand, within BWI76_RS07245at 1525.173 kb on + strand, within BWI76_RS07245at 1525.175 kb on - strand, within BWI76_RS07245at 1525.181 kb on - strand, within BWI76_RS07245at 1525.186 kb on - strand, within BWI76_RS07245at 1525.208 kb on - strand, within BWI76_RS07245at 1525.212 kb on + strand, within BWI76_RS07245at 1525.212 kb on + strand, within BWI76_RS07245at 1525.212 kb on + strand, within BWI76_RS07245at 1525.231 kb on - strand, within BWI76_RS07245at 1525.262 kb on - strand, within BWI76_RS07245at 1525.273 kb on - strand, within BWI76_RS07245at 1525.427 kb on - strand, within BWI76_RS07245at 1525.450 kb on + strand, within BWI76_RS07245at 1525.450 kb on + strand, within BWI76_RS07245at 1525.450 kb on + strand, within BWI76_RS07245at 1525.450 kb on + strand, within BWI76_RS07245at 1525.450 kb on + strand, within BWI76_RS07245at 1525.450 kb on + strand, within BWI76_RS07245at 1525.450 kb on + strand, within BWI76_RS07245at 1525.450 kb on + strand, within BWI76_RS07245at 1525.450 kb on + strand, within BWI76_RS07245at 1525.450 kb on + strand, within BWI76_RS07245at 1525.458 kb on - strand, within BWI76_RS07245at 1525.458 kb on - strand, within BWI76_RS07245at 1525.458 kb on - strandat 1525.458 kb on - strand, within BWI76_RS07245at 1525.458 kb on - strand, within BWI76_RS07245at 1525.458 kb on - strand, within BWI76_RS07245at 1525.458 kb on - strand, within BWI76_RS07245at 1525.499 kb on - strand, within BWI76_RS07245at 1525.552 kb on + strand, within BWI76_RS07245at 1525.552 kb on + strand, within BWI76_RS07245at 1525.735 kb on + strand, within BWI76_RS07245at 1525.866 kb on - strand, within BWI76_RS07245at 1526.001 kb on + strandat 1526.616 kb on + strand, within BWI76_RS07250at 1527.418 kb on - strand, within BWI76_RS07255at 1527.480 kb on + strand, within BWI76_RS07255at 1527.639 kb on + strand, within BWI76_RS07255at 1527.761 kb on - strandat 1527.888 kb on - strand, within BWI76_RS07260at 1528.023 kb on + strand, within BWI76_RS07260at 1528.048 kb on - strand, within BWI76_RS07260

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml
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1,525,065 + BWI76_RS07245 0.12 +0.9
1,525,173 + BWI76_RS07245 0.22 -0.7
1,525,175 - BWI76_RS07245 0.22 -1.0
1,525,181 - BWI76_RS07245 0.23 -0.2
1,525,186 - BWI76_RS07245 0.23 -0.0
1,525,208 - BWI76_RS07245 0.26 -0.9
1,525,212 + BWI76_RS07245 0.26 +0.1
1,525,212 + BWI76_RS07245 0.26 -1.2
1,525,212 + BWI76_RS07245 0.26 -3.4
1,525,231 - BWI76_RS07245 0.28 -0.9
1,525,262 - BWI76_RS07245 0.31 -0.2
1,525,273 - BWI76_RS07245 0.32 -0.7
1,525,427 - BWI76_RS07245 0.47 +0.6
1,525,450 + BWI76_RS07245 0.49 -0.3
1,525,450 + BWI76_RS07245 0.49 -0.1
1,525,450 + BWI76_RS07245 0.49 -0.2
1,525,450 + BWI76_RS07245 0.49 +0.0
1,525,450 + BWI76_RS07245 0.49 -0.0
1,525,450 + BWI76_RS07245 0.49 +0.5
1,525,450 + BWI76_RS07245 0.49 -0.3
1,525,450 + BWI76_RS07245 0.49 -1.1
1,525,450 + BWI76_RS07245 0.49 +0.7
1,525,450 + BWI76_RS07245 0.49 -0.2
1,525,458 - BWI76_RS07245 0.50 +0.1
1,525,458 - BWI76_RS07245 0.50 -0.9
1,525,458 - +0.5
1,525,458 - BWI76_RS07245 0.50 +0.4
1,525,458 - BWI76_RS07245 0.50 -0.6
1,525,458 - BWI76_RS07245 0.50 +0.1
1,525,458 - BWI76_RS07245 0.50 +1.7
1,525,499 - BWI76_RS07245 0.54 +0.2
1,525,552 + BWI76_RS07245 0.59 -0.6
1,525,552 + BWI76_RS07245 0.59 +0.6
1,525,735 + BWI76_RS07245 0.77 -1.1
1,525,866 - BWI76_RS07245 0.89 -0.2
1,526,001 + -0.0
1,526,616 + BWI76_RS07250 0.48 +0.2
1,527,418 - BWI76_RS07255 0.33 -0.8
1,527,480 + BWI76_RS07255 0.46 -0.4
1,527,639 + BWI76_RS07255 0.79 -0.7
1,527,761 - -0.2
1,527,888 - BWI76_RS07260 0.11 +2.7
1,528,023 + BWI76_RS07260 0.23 -0.3
1,528,048 - BWI76_RS07260 0.25 -0.1

Or see this region's nucleotide sequence