Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08800 and BWI76_RS08805 are separated by 111 nucleotides BWI76_RS08805 and BWI76_RS08810 are separated by 28 nucleotides BWI76_RS08810 and BWI76_RS08815 are separated by 111 nucleotides
BWI76_RS08800: BWI76_RS08800 - phosphoethanolamine transferase EptA, at 1,855,514 to 1,857,157
_RS08800
BWI76_RS08805: BWI76_RS08805 - gluconeogenesis factor, at 1,857,269 to 1,858,174
_RS08805
BWI76_RS08810: BWI76_RS08810 - hypothetical protein, at 1,858,203 to 1,858,397
_RS08810
BWI76_RS08815: BWI76_RS08815 - cyclic pyranopterin phosphate synthase, at 1,858,509 to 1,859,508
_RS08815
Position (kb)
1857
1858
1859 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1856.866 kb on + strand, within BWI76_RS08800 at 1857.043 kb on + strand at 1857.130 kb on - strand at 1857.170 kb on + strand at 1857.267 kb on + strand at 1857.274 kb on - strand at 1857.288 kb on + strand at 1857.296 kb on - strand at 1857.313 kb on - strand at 1857.347 kb on + strand at 1857.491 kb on + strand, within BWI76_RS08805 at 1857.550 kb on - strand, within BWI76_RS08805 at 1857.725 kb on + strand, within BWI76_RS08805 at 1857.978 kb on - strand, within BWI76_RS08805 at 1858.072 kb on + strand, within BWI76_RS08805 at 1858.214 kb on + strand at 1858.397 kb on + strand at 1858.397 kb on + strand at 1858.404 kb on - strand at 1858.505 kb on + strand at 1858.513 kb on + strand at 1858.833 kb on + strand, within BWI76_RS08815 at 1859.001 kb on - strand, within BWI76_RS08815 at 1859.011 kb on + strand, within BWI76_RS08815 at 1859.062 kb on + strand, within BWI76_RS08815
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 1,856,866 + BWI76_RS08800 0.82 +0.2 1,857,043 + +0.4 1,857,130 - +2.8 1,857,170 + -0.9 1,857,267 + -1.6 1,857,274 - +0.4 1,857,288 + -0.2 1,857,296 - +1.3 1,857,313 - +0.0 1,857,347 + +0.2 1,857,491 + BWI76_RS08805 0.25 +0.5 1,857,550 - BWI76_RS08805 0.31 -1.0 1,857,725 + BWI76_RS08805 0.50 -0.2 1,857,978 - BWI76_RS08805 0.78 +2.1 1,858,072 + BWI76_RS08805 0.89 -1.0 1,858,214 + +0.5 1,858,397 + -0.6 1,858,397 + +0.1 1,858,404 - -0.2 1,858,505 + +0.1 1,858,513 + +1.7 1,858,833 + BWI76_RS08815 0.32 +0.3 1,859,001 - BWI76_RS08815 0.49 +1.6 1,859,011 + BWI76_RS08815 0.50 +1.8 1,859,062 + BWI76_RS08815 0.55 +2.1
Or see this region's nucleotide sequence