Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02520 and BWI76_RS02525 are separated by 10 nucleotides BWI76_RS02525 and BWI76_RS02530 overlap by 8 nucleotides
BWI76_RS02520: BWI76_RS02520 - N-acetylmuramoyl-L-alanine amidase, at 515,678 to 517,015
_RS02520
BWI76_RS02525: BWI76_RS02525 - DNA mismatch repair protein MutL, at 517,026 to 518,921
_RS02525
BWI76_RS02530: BWI76_RS02530 - tRNA dimethylallyltransferase, at 518,914 to 519,864
_RS02530
Position (kb) 517
518
519 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 516.171 kb on - strand, within BWI76_RS02520 at 516.196 kb on + strand, within BWI76_RS02520 at 516.204 kb on - strand, within BWI76_RS02520 at 516.367 kb on + strand, within BWI76_RS02520 at 516.573 kb on + strand, within BWI76_RS02520 at 516.610 kb on - strand, within BWI76_RS02520 at 516.702 kb on + strand, within BWI76_RS02520 at 516.739 kb on + strand, within BWI76_RS02520 at 516.810 kb on + strand, within BWI76_RS02520 at 517.279 kb on + strand, within BWI76_RS02525 at 517.279 kb on + strand, within BWI76_RS02525 at 517.287 kb on - strand, within BWI76_RS02525 at 517.317 kb on + strand, within BWI76_RS02525 at 517.317 kb on + strand, within BWI76_RS02525 at 517.325 kb on - strand, within BWI76_RS02525 at 517.358 kb on - strand, within BWI76_RS02525 at 517.464 kb on - strand, within BWI76_RS02525 at 517.790 kb on + strand, within BWI76_RS02525 at 517.893 kb on - strand, within BWI76_RS02525 at 517.926 kb on - strand, within BWI76_RS02525 at 518.074 kb on - strand, within BWI76_RS02525 at 518.152 kb on + strand, within BWI76_RS02525 at 518.287 kb on + strand, within BWI76_RS02525 at 518.325 kb on + strand, within BWI76_RS02525 at 518.468 kb on - strand, within BWI76_RS02525 at 518.502 kb on + strand, within BWI76_RS02525 at 518.512 kb on + strand, within BWI76_RS02525 at 518.621 kb on - strand, within BWI76_RS02525 at 518.651 kb on + strand, within BWI76_RS02525 at 518.652 kb on - strand, within BWI76_RS02525 at 518.659 kb on - strand, within BWI76_RS02525 at 518.758 kb on + strand at 518.816 kb on + strand at 518.846 kb on + strand at 518.854 kb on - strand at 518.854 kb on - strand at 518.940 kb on + strand at 519.180 kb on + strand, within BWI76_RS02530 at 519.287 kb on - strand, within BWI76_RS02530 at 519.287 kb on - strand, within BWI76_RS02530 at 519.305 kb on - strand, within BWI76_RS02530 at 519.530 kb on + strand, within BWI76_RS02530 at 519.776 kb on + strand at 519.853 kb on + strand at 519.882 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 516,171 - BWI76_RS02520 0.37 -1.2 516,196 + BWI76_RS02520 0.39 -1.4 516,204 - BWI76_RS02520 0.39 -0.8 516,367 + BWI76_RS02520 0.51 +2.8 516,573 + BWI76_RS02520 0.67 -1.1 516,610 - BWI76_RS02520 0.70 +0.6 516,702 + BWI76_RS02520 0.77 +0.5 516,739 + BWI76_RS02520 0.79 -1.8 516,810 + BWI76_RS02520 0.85 +2.0 517,279 + BWI76_RS02525 0.13 +1.6 517,279 + BWI76_RS02525 0.13 -0.7 517,287 - BWI76_RS02525 0.14 -1.2 517,317 + BWI76_RS02525 0.15 -0.9 517,317 + BWI76_RS02525 0.15 -0.4 517,325 - BWI76_RS02525 0.16 +0.0 517,358 - BWI76_RS02525 0.18 +1.9 517,464 - BWI76_RS02525 0.23 +0.3 517,790 + BWI76_RS02525 0.40 +2.4 517,893 - BWI76_RS02525 0.46 -1.7 517,926 - BWI76_RS02525 0.47 +3.3 518,074 - BWI76_RS02525 0.55 -1.7 518,152 + BWI76_RS02525 0.59 +0.6 518,287 + BWI76_RS02525 0.67 -1.7 518,325 + BWI76_RS02525 0.69 +0.1 518,468 - BWI76_RS02525 0.76 -1.9 518,502 + BWI76_RS02525 0.78 +1.7 518,512 + BWI76_RS02525 0.78 +3.6 518,621 - BWI76_RS02525 0.84 -1.0 518,651 + BWI76_RS02525 0.86 -0.3 518,652 - BWI76_RS02525 0.86 +1.8 518,659 - BWI76_RS02525 0.86 +0.5 518,758 + +0.6 518,816 + -0.8 518,846 + +2.7 518,854 - -0.8 518,854 - -1.2 518,940 + +0.7 519,180 + BWI76_RS02530 0.28 -0.3 519,287 - BWI76_RS02530 0.39 -0.0 519,287 - BWI76_RS02530 0.39 -0.1 519,305 - BWI76_RS02530 0.41 -0.4 519,530 + BWI76_RS02530 0.65 -0.6 519,776 + -2.2 519,853 + -1.5 519,882 + -1.1
Or see this region's nucleotide sequence