Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20285 and BWI76_RS20290 are separated by 208 nucleotides BWI76_RS20290 and BWI76_RS20295 are separated by 65 nucleotides BWI76_RS20295 and BWI76_RS20300 are separated by 23 nucleotides
BWI76_RS20285: BWI76_RS20285 - glutathione S-transferase, at 4,250,307 to 4,250,951
_RS20285
BWI76_RS20290: BWI76_RS20290 - thiol:disulfide oxidoreductase, at 4,251,160 to 4,251,789
_RS20290
BWI76_RS20295: BWI76_RS20295 - dihydroneopterin triphosphate 2'-epimerase, at 4,251,855 to 4,252,217
_RS20295
BWI76_RS20300: BWI76_RS20300 - epimerase, at 4,252,241 to 4,253,134
_RS20300
Position (kb) 4251
4252
4253 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 4250.946 kb on + strand at 4251.047 kb on + strand at 4251.055 kb on - strand at 4251.062 kb on - strand at 4251.082 kb on + strand at 4251.261 kb on - strand, within BWI76_RS20290 at 4251.364 kb on - strand, within BWI76_RS20290 at 4251.451 kb on - strand, within BWI76_RS20290 at 4251.611 kb on + strand, within BWI76_RS20290 at 4251.715 kb on + strand, within BWI76_RS20290 at 4251.715 kb on + strand, within BWI76_RS20290 at 4251.847 kb on + strand at 4252.004 kb on + strand, within BWI76_RS20295 at 4252.130 kb on + strand, within BWI76_RS20295 at 4252.172 kb on + strand, within BWI76_RS20295 at 4252.172 kb on + strand, within BWI76_RS20295 at 4252.180 kb on - strand, within BWI76_RS20295
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 4,250,946 + -3.2 4,251,047 + -0.0 4,251,055 - -0.5 4,251,062 - +0.1 4,251,082 + -1.2 4,251,261 - BWI76_RS20290 0.16 +3.4 4,251,364 - BWI76_RS20290 0.32 +1.1 4,251,451 - BWI76_RS20290 0.46 +0.1 4,251,611 + BWI76_RS20290 0.72 -0.9 4,251,715 + BWI76_RS20290 0.88 -0.1 4,251,715 + BWI76_RS20290 0.88 -1.9 4,251,847 + +0.2 4,252,004 + BWI76_RS20295 0.41 +9.8 4,252,130 + BWI76_RS20295 0.76 +0.1 4,252,172 + BWI76_RS20295 0.87 +0.1 4,252,172 + BWI76_RS20295 0.87 -0.6 4,252,180 - BWI76_RS20295 0.90 +1.1
Or see this region's nucleotide sequence