Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14000 and BWI76_RS14005 are separated by 2 nucleotides BWI76_RS14005 and BWI76_RS14010 are separated by 116 nucleotides BWI76_RS14010 and BWI76_RS14015 are separated by 265 nucleotides
BWI76_RS14000: BWI76_RS14000 - glutamine amidotransferase, at 2,925,897 to 2,926,640
_RS14000
BWI76_RS14005: BWI76_RS14005 - TetR family transcriptional regulator, at 2,926,643 to 2,927,191
_RS14005
BWI76_RS14010: BWI76_RS14010 - SDR family oxidoreductase, at 2,927,308 to 2,928,090
_RS14010
BWI76_RS14015: BWI76_RS14015 - L-2,4-diaminobutyrate decarboxylase, at 2,928,356 to 2,929,828
_RS14015
Position (kb)
2927
2928
2929 Strain fitness (log2 ratio)
-2
-1
0
1 at 2926.339 kb on - strand, within BWI76_RS14000 at 2926.343 kb on + strand, within BWI76_RS14000 at 2926.523 kb on - strand, within BWI76_RS14000 at 2926.570 kb on + strand at 2926.816 kb on + strand, within BWI76_RS14005 at 2927.693 kb on + strand, within BWI76_RS14010 at 2927.773 kb on + strand, within BWI76_RS14010 at 2927.810 kb on + strand, within BWI76_RS14010 at 2927.957 kb on + strand, within BWI76_RS14010 at 2928.097 kb on + strand at 2928.114 kb on + strand at 2929.084 kb on + strand, within BWI76_RS14015
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 2,926,339 - BWI76_RS14000 0.59 -0.6 2,926,343 + BWI76_RS14000 0.60 -0.3 2,926,523 - BWI76_RS14000 0.84 +0.0 2,926,570 + -0.6 2,926,816 + BWI76_RS14005 0.32 -0.6 2,927,693 + BWI76_RS14010 0.49 +0.2 2,927,773 + BWI76_RS14010 0.59 +0.4 2,927,810 + BWI76_RS14010 0.64 +1.4 2,927,957 + BWI76_RS14010 0.83 -2.4 2,928,097 + +0.2 2,928,114 + +0.0 2,929,084 + BWI76_RS14015 0.49 +1.2
Or see this region's nucleotide sequence