Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS07745

Experiment: Paraquat dichloride 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS07740 and BWI76_RS07745 are separated by 370 nucleotidesBWI76_RS07745 and BWI76_RS07750 are separated by 9 nucleotides BWI76_RS07740: BWI76_RS07740 - Fe2+-enterobactin ABC transporter substrate-binding protein, at 1,641,986 to 1,642,945 _RS07740 BWI76_RS07745: BWI76_RS07745 - isochorismate synthase EntC, at 1,643,316 to 1,644,491 _RS07745 BWI76_RS07750: BWI76_RS07750 - 2,3-dihydroxybenzoate-AMP ligase, at 1,644,501 to 1,646,108 _RS07750 Position (kb) 1643 1644 1645Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6at 1642.726 kb on - strand, within BWI76_RS07740at 1643.195 kb on + strandat 1643.579 kb on + strand, within BWI76_RS07745at 1643.587 kb on - strand, within BWI76_RS07745at 1643.596 kb on + strand, within BWI76_RS07745at 1643.670 kb on - strand, within BWI76_RS07745at 1643.848 kb on + strand, within BWI76_RS07745at 1643.848 kb on + strand, within BWI76_RS07745at 1644.263 kb on - strand, within BWI76_RS07745at 1644.795 kb on - strand, within BWI76_RS07750at 1644.809 kb on + strand, within BWI76_RS07750at 1644.844 kb on - strand, within BWI76_RS07750at 1645.289 kb on - strand, within BWI76_RS07750

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Per-strain Table

Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml
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1,642,726 - BWI76_RS07740 0.77 +6.0
1,643,195 + -0.5
1,643,579 + BWI76_RS07745 0.22 +0.2
1,643,587 - BWI76_RS07745 0.23 +0.8
1,643,596 + BWI76_RS07745 0.24 +0.5
1,643,670 - BWI76_RS07745 0.30 +0.6
1,643,848 + BWI76_RS07745 0.45 +0.7
1,643,848 + BWI76_RS07745 0.45 +0.4
1,644,263 - BWI76_RS07745 0.81 +1.0
1,644,795 - BWI76_RS07750 0.18 +0.6
1,644,809 + BWI76_RS07750 0.19 -0.4
1,644,844 - BWI76_RS07750 0.21 +0.8
1,645,289 - BWI76_RS07750 0.49 +2.1

Or see this region's nucleotide sequence