Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04845 and BWI76_RS04850 are separated by 194 nucleotides BWI76_RS04850 and BWI76_RS04855 are separated by 9 nucleotides BWI76_RS04855 and BWI76_RS04860 are separated by 31 nucleotides BWI76_RS04860 and BWI76_RS04865 overlap by 1 nucleotides
BWI76_RS04845: BWI76_RS04845 - nicotinate-nucleotide pyrophosphorylase, at 1,012,016 to 1,012,909
_RS04845
BWI76_RS04850: BWI76_RS04850 - N-acetylmuramoyl-L-alanine amidase, at 1,013,104 to 1,013,655
_RS04850
BWI76_RS04855: BWI76_RS04855 - regulatory protein AmpE, at 1,013,665 to 1,014,519
_RS04855
BWI76_RS04860: BWI76_RS04860 - alpha-N-arabinofuranosidase, at 1,014,551 to 1,015,501
_RS04860
BWI76_RS04865: BWI76_RS04865 - MFS transporter, at 1,015,501 to 1,016,907
_RS04865
Position (kb) 1013
1014
1015 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1012.802 kb on + strand, within BWI76_RS04845 at 1012.802 kb on + strand, within BWI76_RS04845 at 1012.810 kb on - strand, within BWI76_RS04845 at 1013.094 kb on - strand at 1013.256 kb on + strand, within BWI76_RS04850 at 1013.368 kb on + strand, within BWI76_RS04850 at 1013.398 kb on + strand, within BWI76_RS04850 at 1013.472 kb on + strand, within BWI76_RS04850 at 1013.514 kb on - strand at 1013.613 kb on - strand at 1013.675 kb on + strand at 1013.681 kb on - strand at 1013.708 kb on + strand at 1013.716 kb on - strand at 1013.716 kb on - strand at 1013.760 kb on - strand, within BWI76_RS04855 at 1013.784 kb on + strand, within BWI76_RS04855 at 1013.844 kb on + strand, within BWI76_RS04855 at 1013.904 kb on + strand, within BWI76_RS04855 at 1013.904 kb on - strand, within BWI76_RS04855 at 1013.917 kb on + strand at 1014.080 kb on - strand, within BWI76_RS04855 at 1014.278 kb on + strand, within BWI76_RS04855 at 1014.395 kb on - strand, within BWI76_RS04855 at 1014.681 kb on + strand, within BWI76_RS04860 at 1014.735 kb on + strand, within BWI76_RS04860 at 1014.748 kb on - strand, within BWI76_RS04860 at 1014.824 kb on - strand, within BWI76_RS04860 at 1014.841 kb on - strand, within BWI76_RS04860 at 1015.293 kb on - strand, within BWI76_RS04860 at 1015.293 kb on - strand, within BWI76_RS04860 at 1015.366 kb on + strand, within BWI76_RS04860 at 1015.374 kb on - strand, within BWI76_RS04860 at 1015.400 kb on + strand, within BWI76_RS04860 at 1015.493 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 1,012,802 + BWI76_RS04845 0.88 -0.1 1,012,802 + BWI76_RS04845 0.88 -0.4 1,012,810 - BWI76_RS04845 0.89 -2.4 1,013,094 - +1.1 1,013,256 + BWI76_RS04850 0.28 +0.4 1,013,368 + BWI76_RS04850 0.48 -0.3 1,013,398 + BWI76_RS04850 0.53 -0.3 1,013,472 + BWI76_RS04850 0.67 -0.3 1,013,514 - +0.1 1,013,613 - +0.1 1,013,675 + -0.0 1,013,681 - -1.2 1,013,708 + +0.1 1,013,716 - -1.1 1,013,716 - -0.3 1,013,760 - BWI76_RS04855 0.11 -0.5 1,013,784 + BWI76_RS04855 0.14 -0.3 1,013,844 + BWI76_RS04855 0.21 -0.0 1,013,904 + BWI76_RS04855 0.28 +0.5 1,013,904 - BWI76_RS04855 0.28 -0.7 1,013,917 + +1.9 1,014,080 - BWI76_RS04855 0.49 +2.7 1,014,278 + BWI76_RS04855 0.72 +1.4 1,014,395 - BWI76_RS04855 0.85 +0.4 1,014,681 + BWI76_RS04860 0.14 +0.4 1,014,735 + BWI76_RS04860 0.19 +0.0 1,014,748 - BWI76_RS04860 0.21 -2.0 1,014,824 - BWI76_RS04860 0.29 -0.4 1,014,841 - BWI76_RS04860 0.30 +0.6 1,015,293 - BWI76_RS04860 0.78 -0.4 1,015,293 - BWI76_RS04860 0.78 +1.0 1,015,366 + BWI76_RS04860 0.86 -0.6 1,015,374 - BWI76_RS04860 0.87 -0.4 1,015,400 + BWI76_RS04860 0.89 +0.3 1,015,493 + -0.4
Or see this region's nucleotide sequence