Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3322c

Experiment: 7H9 with Sisomicin 0.07 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3319 and Rv3320c are separated by 78 nucleotidesRv3320c and Rv3321c are separated by 3 nucleotidesRv3321c and Rv3322c are separated by 121 nucleotidesRv3322c and Rv3323c overlap by 4 nucleotidesRv3323c and Rv3324c are separated by 0 nucleotides Rv3319: Rv3319 - Probable succinate dehydrogenase (iron-sulphur protein subunit) SdhB (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase), at 3,706,772 to 3,707,563 Rv3319 Rv3320c: Rv3320c - Possible toxin VapC44. Contains PIN domain., at 3,707,642 to 3,708,070 Rv3320c Rv3321c: Rv3321c - Possible antitoxin VapB44, at 3,708,074 to 3,708,316 Rv3321c Rv3322c: Rv3322c - Possible methyltransferase, at 3,708,438 to 3,709,052 Rv3322c Rv3323c: Rv3323c - Probable MoaD-MoaE fusion protein MoaX, at 3,709,049 to 3,709,714 Rv3323c Rv3324c: Rv3324c - Probable molybdenum cofactor biosynthesis protein C 3 MoaC3, at 3,709,715 to 3,710,248 Rv3324c Position (kb) 3708 3709 3710Strain fitness (log2 ratio) -2 -1 0 1at 3707.751 kb on + strand, within Rv3320cat 3708.204 kb on - strand, within Rv3321cat 3708.284 kb on + strand, within Rv3321cat 3708.285 kb on - strand, within Rv3321cat 3708.439 kb on + strandat 3708.439 kb on + strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.501 kb on - strand, within Rv3322cat 3708.516 kb on + strand, within Rv3322cat 3708.516 kb on + strand, within Rv3322cat 3708.630 kb on + strand, within Rv3322cat 3708.630 kb on + strand, within Rv3322cat 3708.630 kb on + strand, within Rv3322cat 3708.630 kb on + strand, within Rv3322cat 3708.630 kb on + strand, within Rv3322cat 3708.669 kb on + strand, within Rv3322cat 3709.320 kb on + strand, within Rv3323cat 3709.320 kb on + strand, within Rv3323cat 3709.321 kb on - strand, within Rv3323cat 3709.321 kb on - strand, within Rv3323cat 3709.323 kb on + strand, within Rv3323cat 3709.323 kb on + strand, within Rv3323cat 3709.324 kb on - strand, within Rv3323cat 3709.357 kb on + strand, within Rv3323cat 3709.357 kb on + strand, within Rv3323cat 3709.458 kb on + strand, within Rv3323cat 3709.459 kb on - strand, within Rv3323cat 3709.487 kb on + strand, within Rv3323cat 3709.487 kb on + strand, within Rv3323cat 3709.693 kb on - strandat 3709.709 kb on - strandat 3709.915 kb on + strand, within Rv3324cat 3709.915 kb on + strand, within Rv3324cat 3709.915 kb on + strand, within Rv3324cat 3709.915 kb on + strand, within Rv3324cat 3709.915 kb on + strand, within Rv3324cat 3709.916 kb on - strand, within Rv3324cat 3709.916 kb on - strand, within Rv3324cat 3709.916 kb on - strand, within Rv3324cat 3710.048 kb on - strand, within Rv3324c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Sisomicin 0.07 mM
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3,707,751 + Rv3320c 0.25 +0.9
3,708,204 - Rv3321c 0.53 -0.5
3,708,284 + Rv3321c 0.86 -0.5
3,708,285 - Rv3321c 0.87 -0.7
3,708,439 + -0.1
3,708,439 + +1.3
3,708,440 - -0.7
3,708,440 - -0.7
3,708,440 - +0.2
3,708,440 - +1.1
3,708,440 - -1.0
3,708,440 - -1.1
3,708,440 - +0.9
3,708,440 - -0.2
3,708,501 - Rv3322c 0.10 -1.2
3,708,516 + Rv3322c 0.13 -0.4
3,708,516 + Rv3322c 0.13 -0.8
3,708,630 + Rv3322c 0.31 -0.1
3,708,630 + Rv3322c 0.31 -1.1
3,708,630 + Rv3322c 0.31 -1.1
3,708,630 + Rv3322c 0.31 -1.0
3,708,630 + Rv3322c 0.31 -1.9
3,708,669 + Rv3322c 0.38 -0.5
3,709,320 + Rv3323c 0.41 +0.6
3,709,320 + Rv3323c 0.41 -0.3
3,709,321 - Rv3323c 0.41 -0.6
3,709,321 - Rv3323c 0.41 -0.5
3,709,323 + Rv3323c 0.41 -0.6
3,709,323 + Rv3323c 0.41 -0.9
3,709,324 - Rv3323c 0.41 -2.6
3,709,357 + Rv3323c 0.46 -0.2
3,709,357 + Rv3323c 0.46 -0.1
3,709,458 + Rv3323c 0.61 +0.1
3,709,459 - Rv3323c 0.62 -0.6
3,709,487 + Rv3323c 0.66 -0.5
3,709,487 + Rv3323c 0.66 +1.7
3,709,693 - -0.9
3,709,709 - +0.5
3,709,915 + Rv3324c 0.37 -0.2
3,709,915 + Rv3324c 0.37 +0.5
3,709,915 + Rv3324c 0.37 +0.7
3,709,915 + Rv3324c 0.37 -0.8
3,709,915 + Rv3324c 0.37 +0.5
3,709,916 - Rv3324c 0.38 +0.0
3,709,916 - Rv3324c 0.38 +1.7
3,709,916 - Rv3324c 0.38 +1.1
3,710,048 - Rv3324c 0.62 -0.3

Or see this region's nucleotide sequence