Experiment: 7H9 with Sisomicin 0.07 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3280 and Rv3281 overlap by 20 nucleotides Rv3281 and Rv3282 overlap by 4 nucleotides Rv3282 and Rv3283 are separated by 40 nucleotides Rv3283 and Rv3284 overlap by 4 nucleotides
Rv3280: Rv3280 - Probable propionyl-CoA carboxylase beta chain 5 AccD5 (pccase) (propanoyl-CoA:carbon dioxide ligase), at 3,662,062 to 3,663,708
Rv3280
Rv3281: Rv3281 - Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (epsilon chain) AccE5, at 3,663,689 to 3,664,222
Rv3281
Rv3282: Rv3282 - Conserved hypothetical protein, at 3,664,219 to 3,664,887
Rv3282
Rv3283: Rv3283 - Probable thiosulfate sulfurtransferase SseA (rhodanese) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase), at 3,664,928 to 3,665,821
Rv3283
Rv3284: Rv3284 - Conserved hypothetical protein, at 3,665,818 to 3,666,249
Rv3284
Position (kb)
3664
3665 Strain fitness (log2 ratio)
-1
0
1 at 3664.564 kb on + strand, within Rv3282 at 3664.678 kb on + strand, within Rv3282 at 3664.886 kb on - strand at 3665.142 kb on - strand, within Rv3283 at 3665.214 kb on - strand, within Rv3283 at 3665.495 kb on - strand, within Rv3283 at 3665.495 kb on - strand, within Rv3283 at 3665.504 kb on + strand, within Rv3283 at 3665.658 kb on - strand, within Rv3283 at 3665.727 kb on + strand, within Rv3283 at 3665.728 kb on - strand, within Rv3283 at 3665.735 kb on + strand at 3665.736 kb on - strand at 3665.817 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Sisomicin 0.07 mM remove 3,664,564 + Rv3282 0.52 -1.5 3,664,678 + Rv3282 0.69 +0.1 3,664,886 - -0.1 3,665,142 - Rv3283 0.24 -1.1 3,665,214 - Rv3283 0.32 +0.1 3,665,495 - Rv3283 0.63 -1.6 3,665,495 - Rv3283 0.63 +1.0 3,665,504 + Rv3283 0.64 -0.4 3,665,658 - Rv3283 0.82 -0.3 3,665,727 + Rv3283 0.89 -0.9 3,665,728 - Rv3283 0.89 +0.8 3,665,735 + +0.3 3,665,736 - +1.7 3,665,817 + -0.6
Or see this region's nucleotide sequence