Experiment: 7H9 with Sisomicin 0.07 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv1420 and Rv1421 overlap by 4 nucleotides Rv1421 and Rv1422 overlap by 4 nucleotides Rv1422 and Rv1423 overlap by 4 nucleotides Rv1423 and Rv1424c are separated by 9 nucleotides
Rv1420: Rv1420 - Probable excinuclease ABC (subunit C-nuclease) UvrC, at 1,594,042 to 1,595,982
Rv1420
Rv1421: Rv1421 - Conserved protein, at 1,595,979 to 1,596,884
Rv1421
Rv1422: Rv1422 - Conserved hypothetical protein, at 1,596,881 to 1,597,909
Rv1422
Rv1423: Rv1423 - Probable transcriptional regulatory protein WhiA, at 1,597,906 to 1,598,883
Rv1423
Rv1424c: Rv1424c - Possible membrane protein, at 1,598,893 to 1,599,654
Rv1424c
Position (kb) 1596
1597
1598 Strain fitness (log2 ratio) -1
0
1
2 at 1596.105 kb on + strand, within Rv1421 at 1596.400 kb on + strand, within Rv1421 at 1596.710 kb on - strand, within Rv1421 at 1596.753 kb on - strand, within Rv1421 at 1596.783 kb on - strand, within Rv1421 at 1596.960 kb on - strand at 1597.485 kb on + strand, within Rv1422 at 1597.486 kb on - strand, within Rv1422 at 1597.770 kb on + strand, within Rv1422 at 1598.895 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Sisomicin 0.07 mM remove 1,596,105 + Rv1421 0.14 -1.0 1,596,400 + Rv1421 0.46 -0.0 1,596,710 - Rv1421 0.81 +0.3 1,596,753 - Rv1421 0.85 -1.3 1,596,783 - Rv1421 0.89 +2.2 1,596,960 - +0.2 1,597,485 + Rv1422 0.59 -1.4 1,597,486 - Rv1422 0.59 +0.6 1,597,770 + Rv1422 0.86 -0.4 1,598,895 - -0.6
Or see this region's nucleotide sequence