Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2498c

Experiment: 7H9 with nitrite 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2497c and Rv2498c are separated by 258 nucleotidesRv2498c and Rv2499c overlap by 4 nucleotidesRv2499c and Rv2500c overlap by 4 nucleotides Rv2497c: Rv2497c - Probable branched-chain keto acid dehydrogenase E1 component, alpha subunit BkdA, at 2,810,993 to 2,812,096 Rv2497c Rv2498c: Rv2498c - Probable citrate (pro-3S)-lyase (beta subunit) CitE (citrase) (citratase) (citritase) (citridesmolase) (citrase aldolase), at 2,812,355 to 2,813,176 Rv2498c Rv2499c: Rv2499c - Possible oxidase regulatory-related protein, at 2,813,173 to 2,813,730 Rv2499c Rv2500c: Rv2500c - Possible acyl-CoA dehydrogenase FadE19 (MMGC), at 2,813,727 to 2,814,911 Rv2500c Position (kb) 2812 2813 2814Strain fitness (log2 ratio) -1 0 1 2at 2811.379 kb on - strand, within Rv2497cat 2811.511 kb on - strand, within Rv2497cat 2811.561 kb on - strand, within Rv2497cat 2811.561 kb on - strand, within Rv2497cat 2811.776 kb on + strand, within Rv2497cat 2811.827 kb on + strand, within Rv2497cat 2811.828 kb on - strand, within Rv2497cat 2811.879 kb on + strand, within Rv2497cat 2811.880 kb on - strand, within Rv2497cat 2811.954 kb on + strand, within Rv2497cat 2811.993 kb on + strandat 2812.161 kb on + strandat 2812.284 kb on - strandat 2812.634 kb on + strand, within Rv2498cat 2812.634 kb on + strand, within Rv2498cat 2812.921 kb on + strand, within Rv2498cat 2812.921 kb on + strand, within Rv2498cat 2812.924 kb on - strand, within Rv2498cat 2812.969 kb on + strand, within Rv2498cat 2812.969 kb on + strand, within Rv2498cat 2813.600 kb on + strand, within Rv2499cat 2813.840 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with nitrite 2.5 mM
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2,811,379 - Rv2497c 0.35 -0.5
2,811,511 - Rv2497c 0.47 -0.0
2,811,561 - Rv2497c 0.51 -1.6
2,811,561 - Rv2497c 0.51 -1.0
2,811,776 + Rv2497c 0.71 -0.8
2,811,827 + Rv2497c 0.76 +1.9
2,811,828 - Rv2497c 0.76 -0.9
2,811,879 + Rv2497c 0.80 +0.3
2,811,880 - Rv2497c 0.80 +0.5
2,811,954 + Rv2497c 0.87 +0.4
2,811,993 + +0.7
2,812,161 + -0.2
2,812,284 - +0.9
2,812,634 + Rv2498c 0.34 -1.5
2,812,634 + Rv2498c 0.34 -0.8
2,812,921 + Rv2498c 0.69 -0.3
2,812,921 + Rv2498c 0.69 -1.4
2,812,924 - Rv2498c 0.69 +0.4
2,812,969 + Rv2498c 0.75 -1.3
2,812,969 + Rv2498c 0.75 +0.0
2,813,600 + Rv2499c 0.77 -1.3
2,813,840 + -0.4

Or see this region's nucleotide sequence