Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proB and proA are separated by 15 nucleotides proA and LU632_RS01195 are separated by 83 nucleotides LU632_RS01195 and LU632_RS01200 are separated by 42 nucleotides
LU632_RS01185: proB - glutamate 5-kinase, at 250,192 to 251,295
proB
LU632_RS01190: proA - glutamate-5-semialdehyde dehydrogenase, at 251,311 to 252,564
proA
LU632_RS01195: LU632_RS01195 - LysE family translocator, at 252,648 to 252,767
_RS01195
LU632_RS01200: LU632_RS01200 - HNH endonuclease, at 252,810 to 253,175
_RS01200
Position (kb) 251
252
253 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 250.416 kb on - strand, within proB at 250.472 kb on - strand, within proB at 250.482 kb on + strand, within proB at 250.482 kb on + strand, within proB at 250.484 kb on + strand, within proB at 250.484 kb on + strand, within proB at 250.485 kb on - strand, within proB at 250.485 kb on - strand, within proB at 250.553 kb on + strand, within proB at 250.553 kb on + strand, within proB at 250.553 kb on + strand, within proB at 250.554 kb on - strand, within proB at 250.575 kb on + strand, within proB at 250.575 kb on + strand, within proB at 250.576 kb on - strand, within proB at 250.576 kb on - strand, within proB at 250.809 kb on - strand, within proB at 250.884 kb on + strand, within proB at 250.885 kb on - strand, within proB at 250.885 kb on - strand, within proB at 250.986 kb on + strand, within proB at 251.182 kb on - strand, within proB at 251.221 kb on - strand at 251.245 kb on - strand at 251.281 kb on - strand at 251.529 kb on - strand, within proA at 251.666 kb on - strand, within proA at 251.685 kb on + strand, within proA at 251.870 kb on + strand, within proA at 251.870 kb on + strand, within proA at 251.870 kb on + strand, within proA at 251.870 kb on + strand, within proA at 251.871 kb on - strand, within proA at 251.871 kb on - strand, within proA at 251.874 kb on + strand, within proA at 251.874 kb on + strand, within proA at 251.874 kb on + strand, within proA at 251.875 kb on - strand, within proA at 251.875 kb on - strand, within proA at 252.039 kb on + strand, within proA at 252.040 kb on - strand, within proA at 252.040 kb on - strand, within proA at 252.346 kb on - strand, within proA at 252.523 kb on + strand at 252.523 kb on + strand at 252.523 kb on + strand at 252.559 kb on - strand at 252.572 kb on - strand at 252.576 kb on + strand at 252.577 kb on - strand at 252.577 kb on - strand at 252.591 kb on + strand at 252.591 kb on + strand at 252.592 kb on - strand at 252.701 kb on - strand, within LU632_RS01195 at 252.719 kb on + strand, within LU632_RS01195 at 252.719 kb on + strand, within LU632_RS01195 at 252.720 kb on - strand, within LU632_RS01195 at 252.726 kb on + strand, within LU632_RS01195 at 252.759 kb on - strand at 252.768 kb on + strand at 252.769 kb on - strand at 252.808 kb on - strand at 252.810 kb on - strand at 252.986 kb on - strand, within LU632_RS01200 at 253.018 kb on - strand, within LU632_RS01200 at 253.018 kb on - strand, within LU632_RS01200 at 253.040 kb on + strand, within LU632_RS01200 at 253.077 kb on - strand, within LU632_RS01200 at 253.077 kb on - strand, within LU632_RS01200 at 253.077 kb on - strand, within LU632_RS01200 at 253.085 kb on + strand, within LU632_RS01200 at 253.086 kb on - strand, within LU632_RS01200 at 253.090 kb on - strand, within LU632_RS01200 at 253.248 kb on + strand at 253.248 kb on + strand at 253.249 kb on - strand at 253.252 kb on + strand at 253.263 kb on + strand at 253.267 kb on + strand at 253.267 kb on + strand at 253.267 kb on + strand at 253.268 kb on - strand at 253.268 kb on - strand at 253.268 kb on - strand at 253.268 kb on - strand at 253.268 kb on - strand at 253.345 kb on + strand at 253.360 kb on + strand at 253.517 kb on + strand at 253.518 kb on - strand at 253.518 kb on - strand at 253.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 250,416 - proB LU632_RS01185 0.20 -2.8 250,472 - proB LU632_RS01185 0.25 -1.5 250,482 + proB LU632_RS01185 0.26 -2.2 250,482 + proB LU632_RS01185 0.26 -2.5 250,484 + proB LU632_RS01185 0.26 -3.4 250,484 + proB LU632_RS01185 0.26 -2.2 250,485 - proB LU632_RS01185 0.27 -4.6 250,485 - proB LU632_RS01185 0.27 -0.8 250,553 + proB LU632_RS01185 0.33 -4.6 250,553 + proB LU632_RS01185 0.33 -4.2 250,553 + proB LU632_RS01185 0.33 -3.5 250,554 - proB LU632_RS01185 0.33 -4.4 250,575 + proB LU632_RS01185 0.35 -2.7 250,575 + proB LU632_RS01185 0.35 -3.0 250,576 - proB LU632_RS01185 0.35 -1.2 250,576 - proB LU632_RS01185 0.35 -3.2 250,809 - proB LU632_RS01185 0.56 -2.4 250,884 + proB LU632_RS01185 0.63 -3.1 250,885 - proB LU632_RS01185 0.63 -3.5 250,885 - proB LU632_RS01185 0.63 -1.8 250,986 + proB LU632_RS01185 0.72 -0.2 251,182 - proB LU632_RS01185 0.90 -1.8 251,221 - -4.0 251,245 - -2.2 251,281 - -1.5 251,529 - proA LU632_RS01190 0.17 -3.4 251,666 - proA LU632_RS01190 0.28 -4.0 251,685 + proA LU632_RS01190 0.30 -3.0 251,870 + proA LU632_RS01190 0.45 -1.2 251,870 + proA LU632_RS01190 0.45 -2.8 251,870 + proA LU632_RS01190 0.45 -1.9 251,870 + proA LU632_RS01190 0.45 -1.8 251,871 - proA LU632_RS01190 0.45 -3.9 251,871 - proA LU632_RS01190 0.45 -2.0 251,874 + proA LU632_RS01190 0.45 -2.0 251,874 + proA LU632_RS01190 0.45 -3.3 251,874 + proA LU632_RS01190 0.45 -2.3 251,875 - proA LU632_RS01190 0.45 -2.7 251,875 - proA LU632_RS01190 0.45 -1.4 252,039 + proA LU632_RS01190 0.58 -2.4 252,040 - proA LU632_RS01190 0.58 -1.2 252,040 - proA LU632_RS01190 0.58 -3.8 252,346 - proA LU632_RS01190 0.83 -2.0 252,523 + -1.9 252,523 + -3.3 252,523 + -2.4 252,559 - -4.0 252,572 - -1.0 252,576 + -0.0 252,577 - -0.8 252,577 - -0.2 252,591 + -0.7 252,591 + -1.5 252,592 - -0.6 252,701 - LU632_RS01195 0.44 +0.3 252,719 + LU632_RS01195 0.59 -0.2 252,719 + LU632_RS01195 0.59 +0.2 252,720 - LU632_RS01195 0.60 +0.2 252,726 + LU632_RS01195 0.65 -0.2 252,759 - +0.0 252,768 + +0.5 252,769 - +2.8 252,808 - -0.2 252,810 - +0.8 252,986 - LU632_RS01200 0.48 +0.3 253,018 - LU632_RS01200 0.57 -0.3 253,018 - LU632_RS01200 0.57 +0.8 253,040 + LU632_RS01200 0.63 -0.0 253,077 - LU632_RS01200 0.73 -0.2 253,077 - LU632_RS01200 0.73 +0.4 253,077 - LU632_RS01200 0.73 -0.3 253,085 + LU632_RS01200 0.75 -0.5 253,086 - LU632_RS01200 0.75 -0.4 253,090 - LU632_RS01200 0.77 -0.7 253,248 + -0.1 253,248 + -0.4 253,249 - +0.5 253,252 + +0.1 253,263 + +0.6 253,267 + -0.3 253,267 + -0.4 253,267 + -0.3 253,268 - +0.6 253,268 - -0.4 253,268 - +1.1 253,268 - +0.6 253,268 - -0.4 253,345 + -0.9 253,360 + +0.1 253,517 + -0.5 253,518 - -1.1 253,518 - -0.9 253,520 - -0.8
Or see this region's nucleotide sequence