Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rodZ and pilW overlap by 11 nucleotides pilW and LU632_RS17815 are separated by 82 nucleotides LU632_RS17815 and ndk are separated by 238 nucleotides
LU632_RS17805: rodZ - cytoskeleton protein RodZ, at 3,353,701 to 3,354,702
rodZ
LU632_RS17810: pilW - type IV pilus biogenesis/stability protein PilW, at 3,354,692 to 3,355,429
pilW
LU632_RS17815: LU632_RS17815 - bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN, at 3,355,512 to 3,356,687
_RS17815
LU632_RS17820: ndk - nucleoside-diphosphate kinase, at 3,356,926 to 3,357,357
ndk
Position (kb)
3355
3356
3357 Strain fitness (log2 ratio)
-2
-1
0
1 at 3354.890 kb on - strand, within pilW at 3355.136 kb on - strand, within pilW at 3355.143 kb on - strand, within pilW at 3355.143 kb on - strand, within pilW at 3355.143 kb on - strand, within pilW at 3355.182 kb on + strand, within pilW at 3355.182 kb on + strand, within pilW at 3355.183 kb on - strand, within pilW at 3355.242 kb on + strand, within pilW at 3355.243 kb on - strand, within pilW at 3355.243 kb on - strand, within pilW at 3355.324 kb on - strand, within pilW at 3355.617 kb on - strand at 3355.635 kb on - strand, within LU632_RS17815 at 3355.643 kb on + strand, within LU632_RS17815 at 3355.644 kb on - strand, within LU632_RS17815 at 3355.647 kb on - strand, within LU632_RS17815 at 3355.649 kb on + strand, within LU632_RS17815 at 3355.831 kb on + strand, within LU632_RS17815 at 3355.907 kb on + strand, within LU632_RS17815 at 3355.925 kb on + strand, within LU632_RS17815 at 3355.925 kb on + strand, within LU632_RS17815 at 3355.926 kb on - strand, within LU632_RS17815 at 3355.944 kb on + strand, within LU632_RS17815 at 3355.944 kb on + strand, within LU632_RS17815 at 3355.945 kb on - strand, within LU632_RS17815 at 3355.945 kb on - strand, within LU632_RS17815 at 3355.966 kb on - strand, within LU632_RS17815 at 3356.118 kb on + strand, within LU632_RS17815 at 3356.119 kb on - strand, within LU632_RS17815 at 3356.344 kb on + strand, within LU632_RS17815 at 3356.345 kb on - strand, within LU632_RS17815 at 3356.345 kb on - strand, within LU632_RS17815 at 3356.378 kb on + strand, within LU632_RS17815 at 3356.379 kb on - strand, within LU632_RS17815 at 3356.387 kb on + strand, within LU632_RS17815 at 3356.388 kb on - strand, within LU632_RS17815 at 3356.477 kb on + strand, within LU632_RS17815 at 3356.478 kb on - strand, within LU632_RS17815 at 3356.518 kb on + strand, within LU632_RS17815 at 3356.687 kb on + strand at 3356.688 kb on - strand at 3356.709 kb on + strand at 3356.710 kb on - strand at 3356.734 kb on + strand at 3356.734 kb on + strand at 3356.735 kb on - strand at 3356.762 kb on - strand at 3356.799 kb on + strand at 3356.801 kb on + strand at 3356.801 kb on + strand at 3356.802 kb on - strand at 3356.831 kb on - strand at 3356.850 kb on - strand at 3356.850 kb on - strand at 3356.870 kb on + strand at 3356.877 kb on - strand at 3356.878 kb on - strand at 3356.928 kb on - strand at 3357.039 kb on - strand, within ndk at 3357.039 kb on - strand, within ndk at 3357.207 kb on - strand, within ndk at 3357.328 kb on + strand at 3357.342 kb on + strand at 3357.352 kb on - strand at 3357.379 kb on + strand at 3357.379 kb on + strand at 3357.380 kb on - strand at 3357.380 kb on - strand at 3357.557 kb on - strand at 3357.573 kb on + strand at 3357.574 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 3,354,890 - pilW LU632_RS17810 0.27 -0.7 3,355,136 - pilW LU632_RS17810 0.60 -0.3 3,355,143 - pilW LU632_RS17810 0.61 -0.1 3,355,143 - pilW LU632_RS17810 0.61 -0.2 3,355,143 - pilW LU632_RS17810 0.61 +0.1 3,355,182 + pilW LU632_RS17810 0.66 +0.0 3,355,182 + pilW LU632_RS17810 0.66 +0.5 3,355,183 - pilW LU632_RS17810 0.67 -0.4 3,355,242 + pilW LU632_RS17810 0.75 +0.3 3,355,243 - pilW LU632_RS17810 0.75 -0.5 3,355,243 - pilW LU632_RS17810 0.75 -0.4 3,355,324 - pilW LU632_RS17810 0.86 -0.6 3,355,617 - -0.4 3,355,635 - LU632_RS17815 0.10 -2.2 3,355,643 + LU632_RS17815 0.11 -0.1 3,355,644 - LU632_RS17815 0.11 +1.0 3,355,647 - LU632_RS17815 0.11 -0.7 3,355,649 + LU632_RS17815 0.12 -0.8 3,355,831 + LU632_RS17815 0.27 +0.7 3,355,907 + LU632_RS17815 0.34 -1.5 3,355,925 + LU632_RS17815 0.35 -0.9 3,355,925 + LU632_RS17815 0.35 -0.2 3,355,926 - LU632_RS17815 0.35 -1.1 3,355,944 + LU632_RS17815 0.37 +0.8 3,355,944 + LU632_RS17815 0.37 -0.5 3,355,945 - LU632_RS17815 0.37 +0.2 3,355,945 - LU632_RS17815 0.37 -0.6 3,355,966 - LU632_RS17815 0.39 -0.2 3,356,118 + LU632_RS17815 0.52 -0.6 3,356,119 - LU632_RS17815 0.52 -0.3 3,356,344 + LU632_RS17815 0.71 -0.6 3,356,345 - LU632_RS17815 0.71 -0.5 3,356,345 - LU632_RS17815 0.71 +0.0 3,356,378 + LU632_RS17815 0.74 +0.3 3,356,379 - LU632_RS17815 0.74 -0.1 3,356,387 + LU632_RS17815 0.74 +1.0 3,356,388 - LU632_RS17815 0.74 -0.2 3,356,477 + LU632_RS17815 0.82 -0.3 3,356,478 - LU632_RS17815 0.82 +0.0 3,356,518 + LU632_RS17815 0.86 -0.4 3,356,687 + +0.0 3,356,688 - +0.3 3,356,709 + -0.4 3,356,710 - -0.5 3,356,734 + -0.2 3,356,734 + -0.5 3,356,735 - -0.0 3,356,762 - -0.8 3,356,799 + +1.7 3,356,801 + -1.6 3,356,801 + -1.5 3,356,802 - -0.1 3,356,831 - +0.0 3,356,850 - +0.8 3,356,850 - +0.1 3,356,870 + +0.5 3,356,877 - -0.2 3,356,878 - -0.1 3,356,928 - -0.8 3,357,039 - ndk LU632_RS17820 0.26 +0.4 3,357,039 - ndk LU632_RS17820 0.26 +0.9 3,357,207 - ndk LU632_RS17820 0.65 +0.7 3,357,328 + +0.1 3,357,342 + +0.6 3,357,352 - +1.0 3,357,379 + +0.2 3,357,379 + -0.3 3,357,380 - +1.7 3,357,380 - -0.2 3,357,557 - +1.3 3,357,573 + -0.2 3,357,574 - +0.2
Or see this region's nucleotide sequence