Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1331 and Pf6N2E2_1332 are separated by 12 nucleotides Pf6N2E2_1332 and Pf6N2E2_1333 are separated by 69 nucleotides
Pf6N2E2_1331 - General substrate transporter:Major facilitator superfamily, at 1,566,485 to 1,567,741
_1331
Pf6N2E2_1332 - Protein involved in meta-pathway of phenol degradation, at 1,567,754 to 1,568,686
_1332
Pf6N2E2_1333 - Ethanolamine operon regulatory protein, at 1,568,756 to 1,569,748
_1333
Position (kb)
1567
1568
1569 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1566.808 kb on - strand, within Pf6N2E2_1331 at 1566.808 kb on - strand, within Pf6N2E2_1331 at 1567.194 kb on + strand, within Pf6N2E2_1331 at 1567.195 kb on - strand, within Pf6N2E2_1331 at 1567.195 kb on - strand, within Pf6N2E2_1331 at 1567.323 kb on + strand, within Pf6N2E2_1331 at 1567.324 kb on - strand, within Pf6N2E2_1331 at 1567.447 kb on - strand, within Pf6N2E2_1331 at 1567.755 kb on + strand at 1567.772 kb on - strand at 1567.786 kb on + strand at 1567.824 kb on + strand at 1567.824 kb on + strand at 1567.824 kb on + strand at 1567.825 kb on - strand at 1567.863 kb on + strand, within Pf6N2E2_1332 at 1567.863 kb on + strand, within Pf6N2E2_1332 at 1567.863 kb on + strand, within Pf6N2E2_1332 at 1567.863 kb on + strand, within Pf6N2E2_1332 at 1567.864 kb on - strand, within Pf6N2E2_1332 at 1567.864 kb on - strand, within Pf6N2E2_1332 at 1567.948 kb on - strand, within Pf6N2E2_1332 at 1567.950 kb on + strand, within Pf6N2E2_1332 at 1567.950 kb on + strand, within Pf6N2E2_1332 at 1567.951 kb on - strand, within Pf6N2E2_1332 at 1568.031 kb on + strand, within Pf6N2E2_1332 at 1568.031 kb on + strand, within Pf6N2E2_1332 at 1568.032 kb on - strand, within Pf6N2E2_1332 at 1568.106 kb on + strand, within Pf6N2E2_1332 at 1568.107 kb on - strand, within Pf6N2E2_1332 at 1568.107 kb on - strand, within Pf6N2E2_1332 at 1568.373 kb on + strand, within Pf6N2E2_1332 at 1568.373 kb on + strand, within Pf6N2E2_1332 at 1568.373 kb on + strand, within Pf6N2E2_1332 at 1568.373 kb on + strand, within Pf6N2E2_1332 at 1568.373 kb on + strand, within Pf6N2E2_1332 at 1568.374 kb on - strand, within Pf6N2E2_1332 at 1568.412 kb on + strand, within Pf6N2E2_1332 at 1568.412 kb on + strand, within Pf6N2E2_1332 at 1568.413 kb on - strand, within Pf6N2E2_1332 at 1568.413 kb on - strand, within Pf6N2E2_1332 at 1568.413 kb on - strand, within Pf6N2E2_1332 at 1568.497 kb on - strand, within Pf6N2E2_1332 at 1568.497 kb on - strand, within Pf6N2E2_1332 at 1568.506 kb on + strand, within Pf6N2E2_1332 at 1568.507 kb on - strand, within Pf6N2E2_1332 at 1568.507 kb on - strand, within Pf6N2E2_1332 at 1568.507 kb on - strand, within Pf6N2E2_1332 at 1568.565 kb on + strand, within Pf6N2E2_1332 at 1568.565 kb on + strand, within Pf6N2E2_1332 at 1568.566 kb on - strand, within Pf6N2E2_1332 at 1568.651 kb on - strand at 1568.651 kb on - strand at 1568.937 kb on + strand, within Pf6N2E2_1333 at 1568.937 kb on + strand, within Pf6N2E2_1333 at 1569.057 kb on + strand, within Pf6N2E2_1333 at 1569.057 kb on + strand, within Pf6N2E2_1333 at 1569.058 kb on - strand, within Pf6N2E2_1333 at 1569.137 kb on + strand, within Pf6N2E2_1333 at 1569.138 kb on - strand, within Pf6N2E2_1333 at 1569.403 kb on + strand, within Pf6N2E2_1333 at 1569.422 kb on + strand, within Pf6N2E2_1333 at 1569.422 kb on + strand, within Pf6N2E2_1333 at 1569.622 kb on - strand, within Pf6N2E2_1333
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 1,566,808 - Pf6N2E2_1331 0.26 +0.3 1,566,808 - Pf6N2E2_1331 0.26 +0.3 1,567,194 + Pf6N2E2_1331 0.56 +0.1 1,567,195 - Pf6N2E2_1331 0.56 -1.1 1,567,195 - Pf6N2E2_1331 0.56 +0.4 1,567,323 + Pf6N2E2_1331 0.67 +1.7 1,567,324 - Pf6N2E2_1331 0.67 +0.4 1,567,447 - Pf6N2E2_1331 0.77 -1.1 1,567,755 + -0.2 1,567,772 - +2.5 1,567,786 + +0.7 1,567,824 + +0.3 1,567,824 + -0.8 1,567,824 + -0.9 1,567,825 - -0.2 1,567,863 + Pf6N2E2_1332 0.12 +0.8 1,567,863 + Pf6N2E2_1332 0.12 -0.2 1,567,863 + Pf6N2E2_1332 0.12 -1.2 1,567,863 + Pf6N2E2_1332 0.12 +0.4 1,567,864 - Pf6N2E2_1332 0.12 -0.3 1,567,864 - Pf6N2E2_1332 0.12 -0.3 1,567,948 - Pf6N2E2_1332 0.21 +0.1 1,567,950 + Pf6N2E2_1332 0.21 -0.3 1,567,950 + Pf6N2E2_1332 0.21 -0.4 1,567,951 - Pf6N2E2_1332 0.21 +1.4 1,568,031 + Pf6N2E2_1332 0.30 -0.1 1,568,031 + Pf6N2E2_1332 0.30 -1.1 1,568,032 - Pf6N2E2_1332 0.30 -0.1 1,568,106 + Pf6N2E2_1332 0.38 -1.1 1,568,107 - Pf6N2E2_1332 0.38 -0.2 1,568,107 - Pf6N2E2_1332 0.38 +0.4 1,568,373 + Pf6N2E2_1332 0.66 -0.3 1,568,373 + Pf6N2E2_1332 0.66 -0.6 1,568,373 + Pf6N2E2_1332 0.66 +0.3 1,568,373 + Pf6N2E2_1332 0.66 +0.3 1,568,373 + Pf6N2E2_1332 0.66 -0.7 1,568,374 - Pf6N2E2_1332 0.66 -0.3 1,568,412 + Pf6N2E2_1332 0.71 -1.9 1,568,412 + Pf6N2E2_1332 0.71 -0.6 1,568,413 - Pf6N2E2_1332 0.71 +1.0 1,568,413 - Pf6N2E2_1332 0.71 +0.7 1,568,413 - Pf6N2E2_1332 0.71 +0.4 1,568,497 - Pf6N2E2_1332 0.80 +0.7 1,568,497 - Pf6N2E2_1332 0.80 -0.9 1,568,506 + Pf6N2E2_1332 0.81 -0.5 1,568,507 - Pf6N2E2_1332 0.81 -1.5 1,568,507 - Pf6N2E2_1332 0.81 +0.3 1,568,507 - Pf6N2E2_1332 0.81 -1.1 1,568,565 + Pf6N2E2_1332 0.87 -0.2 1,568,565 + Pf6N2E2_1332 0.87 +0.1 1,568,566 - Pf6N2E2_1332 0.87 -0.7 1,568,651 - -0.3 1,568,651 - -0.4 1,568,937 + Pf6N2E2_1333 0.18 +0.9 1,568,937 + Pf6N2E2_1333 0.18 +1.6 1,569,057 + Pf6N2E2_1333 0.30 -0.6 1,569,057 + Pf6N2E2_1333 0.30 +0.2 1,569,058 - Pf6N2E2_1333 0.30 +0.3 1,569,137 + Pf6N2E2_1333 0.38 -1.1 1,569,138 - Pf6N2E2_1333 0.38 -0.0 1,569,403 + Pf6N2E2_1333 0.65 +0.9 1,569,422 + Pf6N2E2_1333 0.67 +0.4 1,569,422 + Pf6N2E2_1333 0.67 +0.1 1,569,622 - Pf6N2E2_1333 0.87 -0.3
Or see this region's nucleotide sequence