Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4325 and Pf6N2E2_4326 are separated by 504 nucleotides Pf6N2E2_4326 and Pf6N2E2_4327 are separated by 158 nucleotides
Pf6N2E2_4325 - hypothetical protein, at 5,045,283 to 5,045,816
_4325
Pf6N2E2_4326 - VgrG protein, at 5,046,321 to 5,048,261
_4326
Pf6N2E2_4327 - ClpB protein, at 5,048,420 to 5,051,110
_4327
Position (kb)
5046
5047
5048
5049 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5045.807 kb on + strand at 5045.841 kb on + strand at 5045.841 kb on + strand at 5045.841 kb on + strand at 5045.842 kb on - strand at 5045.842 kb on - strand at 5045.842 kb on - strand at 5045.842 kb on - strand at 5045.889 kb on - strand at 5045.945 kb on + strand at 5045.945 kb on + strand at 5045.945 kb on + strand at 5045.945 kb on + strand at 5045.945 kb on + strand at 5045.946 kb on - strand at 5045.946 kb on - strand at 5045.946 kb on - strand at 5045.946 kb on - strand at 5045.946 kb on - strand at 5045.946 kb on - strand at 5045.946 kb on - strand at 5045.949 kb on + strand at 5045.949 kb on + strand at 5045.949 kb on + strand at 5045.950 kb on - strand at 5045.950 kb on - strand at 5045.989 kb on + strand at 5045.989 kb on + strand at 5045.989 kb on + strand at 5045.990 kb on - strand at 5046.096 kb on + strand at 5046.096 kb on + strand at 5046.097 kb on - strand at 5046.138 kb on - strand at 5046.469 kb on + strand at 5046.470 kb on - strand at 5046.470 kb on - strand at 5046.470 kb on - strand at 5046.473 kb on + strand at 5046.473 kb on + strand at 5046.473 kb on + strand at 5046.474 kb on - strand at 5046.495 kb on - strand at 5046.497 kb on + strand at 5046.497 kb on + strand at 5046.498 kb on - strand at 5046.574 kb on + strand, within Pf6N2E2_4326 at 5046.575 kb on - strand, within Pf6N2E2_4326 at 5046.575 kb on - strand, within Pf6N2E2_4326 at 5046.881 kb on - strand, within Pf6N2E2_4326 at 5046.904 kb on + strand, within Pf6N2E2_4326 at 5046.904 kb on + strand, within Pf6N2E2_4326 at 5047.286 kb on - strand, within Pf6N2E2_4326 at 5047.286 kb on - strand, within Pf6N2E2_4326 at 5047.286 kb on - strand, within Pf6N2E2_4326 at 5047.286 kb on - strand, within Pf6N2E2_4326 at 5047.310 kb on - strand, within Pf6N2E2_4326 at 5047.310 kb on - strand, within Pf6N2E2_4326 at 5047.441 kb on + strand, within Pf6N2E2_4326 at 5047.442 kb on - strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.568 kb on - strand, within Pf6N2E2_4326 at 5047.625 kb on + strand, within Pf6N2E2_4326 at 5047.625 kb on + strand, within Pf6N2E2_4326 at 5047.626 kb on - strand, within Pf6N2E2_4326 at 5047.626 kb on - strand, within Pf6N2E2_4326 at 5047.661 kb on - strand, within Pf6N2E2_4326 at 5047.663 kb on + strand, within Pf6N2E2_4326 at 5047.663 kb on + strand, within Pf6N2E2_4326 at 5047.663 kb on + strand, within Pf6N2E2_4326 at 5047.757 kb on - strand, within Pf6N2E2_4326 at 5047.757 kb on - strand, within Pf6N2E2_4326 at 5047.760 kb on - strand, within Pf6N2E2_4326 at 5047.760 kb on - strand, within Pf6N2E2_4326 at 5047.813 kb on + strand, within Pf6N2E2_4326 at 5047.825 kb on + strand, within Pf6N2E2_4326 at 5047.825 kb on + strand, within Pf6N2E2_4326 at 5047.840 kb on + strand, within Pf6N2E2_4326 at 5048.149 kb on + strand at 5048.256 kb on + strand at 5048.279 kb on + strand at 5048.336 kb on + strand at 5048.336 kb on + strand at 5048.442 kb on + strand at 5048.443 kb on - strand at 5048.724 kb on + strand, within Pf6N2E2_4327 at 5048.725 kb on - strand, within Pf6N2E2_4327 at 5049.012 kb on + strand, within Pf6N2E2_4327 at 5049.013 kb on - strand, within Pf6N2E2_4327 at 5049.066 kb on + strand, within Pf6N2E2_4327 at 5049.066 kb on + strand, within Pf6N2E2_4327 at 5049.156 kb on + strand, within Pf6N2E2_4327 at 5049.156 kb on + strand, within Pf6N2E2_4327 at 5049.156 kb on + strand, within Pf6N2E2_4327 at 5049.157 kb on - strand, within Pf6N2E2_4327 at 5049.157 kb on - strand, within Pf6N2E2_4327
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 5,045,807 + -0.3 5,045,841 + -1.1 5,045,841 + +1.7 5,045,841 + +0.1 5,045,842 - +1.0 5,045,842 - -1.6 5,045,842 - +1.3 5,045,842 - +0.4 5,045,889 - -2.0 5,045,945 + -2.9 5,045,945 + +1.0 5,045,945 + -2.3 5,045,945 + -2.3 5,045,945 + +2.2 5,045,946 - -0.0 5,045,946 - -1.0 5,045,946 - +2.4 5,045,946 - +0.5 5,045,946 - +1.3 5,045,946 - -1.6 5,045,946 - +1.3 5,045,949 + -2.7 5,045,949 + -1.2 5,045,949 + -2.6 5,045,950 - +0.4 5,045,950 - +0.2 5,045,989 + -0.8 5,045,989 + +2.2 5,045,989 + +0.5 5,045,990 - -2.1 5,046,096 + +0.2 5,046,096 + +0.4 5,046,097 - +1.5 5,046,138 - +2.1 5,046,469 + -0.1 5,046,470 - -0.8 5,046,470 - -3.5 5,046,470 - +0.9 5,046,473 + -1.5 5,046,473 + +2.4 5,046,473 + -2.8 5,046,474 - +1.3 5,046,495 - +0.8 5,046,497 + -0.1 5,046,497 + -1.5 5,046,498 - +1.5 5,046,574 + Pf6N2E2_4326 0.13 -0.4 5,046,575 - Pf6N2E2_4326 0.13 -0.5 5,046,575 - Pf6N2E2_4326 0.13 -3.5 5,046,881 - Pf6N2E2_4326 0.29 -2.9 5,046,904 + Pf6N2E2_4326 0.30 +2.9 5,046,904 + Pf6N2E2_4326 0.30 +0.6 5,047,286 - Pf6N2E2_4326 0.50 +2.0 5,047,286 - Pf6N2E2_4326 0.50 -3.0 5,047,286 - Pf6N2E2_4326 0.50 -2.6 5,047,286 - Pf6N2E2_4326 0.50 +0.5 5,047,310 - Pf6N2E2_4326 0.51 +0.5 5,047,310 - Pf6N2E2_4326 0.51 -2.2 5,047,441 + Pf6N2E2_4326 0.58 +1.7 5,047,442 - Pf6N2E2_4326 0.58 -1.8 5,047,468 + Pf6N2E2_4326 0.59 -0.7 5,047,468 + Pf6N2E2_4326 0.59 -3.1 5,047,468 + Pf6N2E2_4326 0.59 +0.8 5,047,468 + Pf6N2E2_4326 0.59 -0.4 5,047,468 + Pf6N2E2_4326 0.59 -1.6 5,047,468 + Pf6N2E2_4326 0.59 +0.7 5,047,468 + Pf6N2E2_4326 0.59 -0.5 5,047,468 + Pf6N2E2_4326 0.59 -1.1 5,047,469 - Pf6N2E2_4326 0.59 +0.2 5,047,469 - Pf6N2E2_4326 0.59 +0.2 5,047,469 - Pf6N2E2_4326 0.59 -1.3 5,047,469 - Pf6N2E2_4326 0.59 -1.3 5,047,469 - Pf6N2E2_4326 0.59 -2.7 5,047,469 - Pf6N2E2_4326 0.59 -0.3 5,047,469 - Pf6N2E2_4326 0.59 -0.5 5,047,469 - Pf6N2E2_4326 0.59 +0.4 5,047,469 - Pf6N2E2_4326 0.59 -2.3 5,047,568 - Pf6N2E2_4326 0.64 -1.9 5,047,625 + Pf6N2E2_4326 0.67 -0.1 5,047,625 + Pf6N2E2_4326 0.67 -0.8 5,047,626 - Pf6N2E2_4326 0.67 +1.7 5,047,626 - Pf6N2E2_4326 0.67 -1.8 5,047,661 - Pf6N2E2_4326 0.69 -0.6 5,047,663 + Pf6N2E2_4326 0.69 +2.4 5,047,663 + Pf6N2E2_4326 0.69 -1.3 5,047,663 + Pf6N2E2_4326 0.69 -2.7 5,047,757 - Pf6N2E2_4326 0.74 -3.0 5,047,757 - Pf6N2E2_4326 0.74 -1.5 5,047,760 - Pf6N2E2_4326 0.74 -1.8 5,047,760 - Pf6N2E2_4326 0.74 -1.5 5,047,813 + Pf6N2E2_4326 0.77 -1.9 5,047,825 + Pf6N2E2_4326 0.77 -0.3 5,047,825 + Pf6N2E2_4326 0.77 -2.5 5,047,840 + Pf6N2E2_4326 0.78 +1.6 5,048,149 + -2.3 5,048,256 + -2.4 5,048,279 + -0.0 5,048,336 + +1.4 5,048,336 + -2.3 5,048,442 + -2.1 5,048,443 - -2.4 5,048,724 + Pf6N2E2_4327 0.11 -2.3 5,048,725 - Pf6N2E2_4327 0.11 +2.0 5,049,012 + Pf6N2E2_4327 0.22 -0.1 5,049,013 - Pf6N2E2_4327 0.22 +0.7 5,049,066 + Pf6N2E2_4327 0.24 +1.3 5,049,066 + Pf6N2E2_4327 0.24 -1.0 5,049,156 + Pf6N2E2_4327 0.27 -1.9 5,049,156 + Pf6N2E2_4327 0.27 -0.8 5,049,156 + Pf6N2E2_4327 0.27 +1.1 5,049,157 - Pf6N2E2_4327 0.27 +0.3 5,049,157 - Pf6N2E2_4327 0.27 +0.4
Or see this region's nucleotide sequence