Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1781 and Pf6N2E2_1782 are separated by 16 nucleotides Pf6N2E2_1782 and Pf6N2E2_1783 are separated by 32 nucleotides Pf6N2E2_1783 and Pf6N2E2_1784 are separated by 11 nucleotides Pf6N2E2_1784 and Pf6N2E2_1785 are separated by 315 nucleotides
Pf6N2E2_1781 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 2,106,229 to 2,108,358
_1781
Pf6N2E2_1782 - STAS domain protein, at 2,108,375 to 2,108,692
_1782
Pf6N2E2_1783 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 2,108,725 to 2,109,093
_1783
Pf6N2E2_1784 - Methyl-accepting chemotaxis protein, at 2,109,105 to 2,110,223
_1784
Pf6N2E2_1785 - HDIG domain protein, at 2,110,539 to 2,111,780
_1785
Position (kb)
2109
2110
2111 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2108.221 kb on - strand at 2108.339 kb on - strand at 2108.631 kb on + strand, within Pf6N2E2_1782 at 2108.632 kb on - strand, within Pf6N2E2_1782 at 2108.632 kb on - strand, within Pf6N2E2_1782 at 2108.632 kb on - strand, within Pf6N2E2_1782 at 2108.727 kb on - strand at 2108.873 kb on + strand, within Pf6N2E2_1783 at 2108.942 kb on - strand, within Pf6N2E2_1783 at 2108.942 kb on - strand, within Pf6N2E2_1783 at 2109.107 kb on - strand at 2109.131 kb on + strand at 2109.131 kb on + strand at 2109.132 kb on - strand at 2109.132 kb on - strand at 2109.184 kb on + strand at 2109.184 kb on + strand at 2109.185 kb on - strand at 2109.765 kb on + strand, within Pf6N2E2_1784 at 2109.766 kb on - strand, within Pf6N2E2_1784 at 2110.008 kb on + strand, within Pf6N2E2_1784 at 2110.111 kb on - strand, within Pf6N2E2_1784 at 2110.417 kb on - strand at 2110.417 kb on - strand at 2110.422 kb on + strand at 2110.423 kb on - strand at 2110.423 kb on - strand at 2110.423 kb on - strand at 2110.516 kb on - strand at 2110.661 kb on + strand at 2110.661 kb on + strand at 2110.662 kb on - strand at 2110.662 kb on - strand at 2110.700 kb on - strand, within Pf6N2E2_1785 at 2110.729 kb on + strand, within Pf6N2E2_1785 at 2110.729 kb on + strand, within Pf6N2E2_1785 at 2110.798 kb on + strand, within Pf6N2E2_1785 at 2110.799 kb on - strand, within Pf6N2E2_1785 at 2110.802 kb on + strand, within Pf6N2E2_1785 at 2110.802 kb on + strand, within Pf6N2E2_1785 at 2110.803 kb on - strand, within Pf6N2E2_1785 at 2110.803 kb on - strand, within Pf6N2E2_1785 at 2110.803 kb on - strand, within Pf6N2E2_1785 at 2110.803 kb on - strand, within Pf6N2E2_1785 at 2110.803 kb on - strand, within Pf6N2E2_1785 at 2110.803 kb on - strand, within Pf6N2E2_1785 at 2110.803 kb on - strand, within Pf6N2E2_1785 at 2110.803 kb on - strand, within Pf6N2E2_1785 at 2110.930 kb on + strand, within Pf6N2E2_1785 at 2110.931 kb on - strand, within Pf6N2E2_1785 at 2110.983 kb on + strand, within Pf6N2E2_1785 at 2110.983 kb on + strand, within Pf6N2E2_1785 at 2110.984 kb on - strand, within Pf6N2E2_1785 at 2110.984 kb on - strand, within Pf6N2E2_1785 at 2110.984 kb on - strand, within Pf6N2E2_1785 at 2110.984 kb on - strand, within Pf6N2E2_1785 at 2110.984 kb on - strand, within Pf6N2E2_1785 at 2110.989 kb on - strand, within Pf6N2E2_1785 at 2110.989 kb on - strand, within Pf6N2E2_1785 at 2111.059 kb on + strand, within Pf6N2E2_1785 at 2111.060 kb on - strand, within Pf6N2E2_1785 at 2111.215 kb on - strand, within Pf6N2E2_1785
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 2,108,221 - -2.3 2,108,339 - -0.9 2,108,631 + Pf6N2E2_1782 0.81 -0.6 2,108,632 - Pf6N2E2_1782 0.81 -0.5 2,108,632 - Pf6N2E2_1782 0.81 +0.1 2,108,632 - Pf6N2E2_1782 0.81 +1.7 2,108,727 - -0.3 2,108,873 + Pf6N2E2_1783 0.40 +2.3 2,108,942 - Pf6N2E2_1783 0.59 +0.5 2,108,942 - Pf6N2E2_1783 0.59 +2.8 2,109,107 - +0.7 2,109,131 + -1.7 2,109,131 + +1.2 2,109,132 - +0.6 2,109,132 - +1.8 2,109,184 + +1.6 2,109,184 + -0.6 2,109,185 - +0.5 2,109,765 + Pf6N2E2_1784 0.59 -0.3 2,109,766 - Pf6N2E2_1784 0.59 -2.2 2,110,008 + Pf6N2E2_1784 0.81 -0.5 2,110,111 - Pf6N2E2_1784 0.90 -2.0 2,110,417 - -1.1 2,110,417 - -2.2 2,110,422 + +2.1 2,110,423 - +0.1 2,110,423 - -2.3 2,110,423 - +1.3 2,110,516 - -1.5 2,110,661 + -1.5 2,110,661 + +0.7 2,110,662 - -1.6 2,110,662 - +1.7 2,110,700 - Pf6N2E2_1785 0.13 -0.6 2,110,729 + Pf6N2E2_1785 0.15 -0.3 2,110,729 + Pf6N2E2_1785 0.15 -0.3 2,110,798 + Pf6N2E2_1785 0.21 +0.1 2,110,799 - Pf6N2E2_1785 0.21 -0.8 2,110,802 + Pf6N2E2_1785 0.21 +1.0 2,110,802 + Pf6N2E2_1785 0.21 +0.8 2,110,803 - Pf6N2E2_1785 0.21 +3.6 2,110,803 - Pf6N2E2_1785 0.21 +0.8 2,110,803 - Pf6N2E2_1785 0.21 -0.5 2,110,803 - Pf6N2E2_1785 0.21 +1.2 2,110,803 - Pf6N2E2_1785 0.21 +2.0 2,110,803 - Pf6N2E2_1785 0.21 -1.6 2,110,803 - Pf6N2E2_1785 0.21 +3.0 2,110,803 - Pf6N2E2_1785 0.21 -2.7 2,110,930 + Pf6N2E2_1785 0.31 +0.8 2,110,931 - Pf6N2E2_1785 0.32 -3.4 2,110,983 + Pf6N2E2_1785 0.36 +1.6 2,110,983 + Pf6N2E2_1785 0.36 -2.7 2,110,984 - Pf6N2E2_1785 0.36 -3.1 2,110,984 - Pf6N2E2_1785 0.36 +0.9 2,110,984 - Pf6N2E2_1785 0.36 -0.9 2,110,984 - Pf6N2E2_1785 0.36 +2.5 2,110,984 - Pf6N2E2_1785 0.36 +0.5 2,110,989 - Pf6N2E2_1785 0.36 -1.5 2,110,989 - Pf6N2E2_1785 0.36 +1.2 2,111,059 + Pf6N2E2_1785 0.42 +0.7 2,111,060 - Pf6N2E2_1785 0.42 +0.6 2,111,215 - Pf6N2E2_1785 0.54 +0.2
Or see this region's nucleotide sequence