Experiment: Nickel (II) chloride 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5129 and Pf6N2E2_6146 are separated by 415 nucleotides Pf6N2E2_6146 and Pf6N2E2_6147 are separated by 10 nucleotides Pf6N2E2_6147 and Pf6N2E2_6148 are separated by 8 nucleotides Pf6N2E2_6148 and Pf6N2E2_6149 are separated by 96 nucleotides Pf6N2E2_6149 and Pf6N2E2_5130 overlap by 9 nucleotides Pf6N2E2_5130 and Pf6N2E2_5131 are separated by 30 nucleotides Pf6N2E2_5131 and Pf6N2E2_5132 are separated by 139 nucleotides
Pf6N2E2_5129 - ClpB protein, at 5,913,135 to 5,915,699
_5129
Pf6N2E2_6146 - tRNA-Asn-GTT, at 5,916,115 to 5,916,187
_6146
Pf6N2E2_6147 - tRNA-Pro-TGG, at 5,916,198 to 5,916,271
_6147
Pf6N2E2_6148 - tRNA-Lys-TTT, at 5,916,280 to 5,916,352
_6148
Pf6N2E2_6149 - tRNA-Pro-TGG, at 5,916,449 to 5,916,522
_6149
Pf6N2E2_5130 - hypothetical protein, at 5,916,514 to 5,916,642
_5130
Pf6N2E2_5131 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 5,916,673 to 5,918,298
_5131
Pf6N2E2_5132 - NADH dehydrogenase (EC 1.6.99.3), at 5,918,438 to 5,919,736
_5132
Position (kb) 5916
5917
5918
5919 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5915.936 kb on + strand at 5915.936 kb on + strand at 5916.387 kb on + strand at 5916.526 kb on + strand at 5916.526 kb on + strand at 5916.526 kb on + strand at 5916.541 kb on + strand, within Pf6N2E2_5130 at 5916.541 kb on + strand, within Pf6N2E2_5130 at 5916.551 kb on + strand, within Pf6N2E2_5130 at 5916.657 kb on - strand at 5916.672 kb on + strand at 5916.913 kb on - strand, within Pf6N2E2_5131 at 5916.913 kb on - strand, within Pf6N2E2_5131 at 5916.922 kb on + strand, within Pf6N2E2_5131 at 5917.015 kb on + strand, within Pf6N2E2_5131 at 5917.015 kb on + strand, within Pf6N2E2_5131 at 5917.046 kb on - strand, within Pf6N2E2_5131 at 5917.225 kb on + strand, within Pf6N2E2_5131 at 5917.226 kb on - strand, within Pf6N2E2_5131 at 5917.311 kb on + strand, within Pf6N2E2_5131 at 5917.338 kb on + strand, within Pf6N2E2_5131 at 5917.339 kb on - strand, within Pf6N2E2_5131 at 5917.339 kb on - strand, within Pf6N2E2_5131 at 5918.034 kb on + strand, within Pf6N2E2_5131 at 5918.300 kb on + strand at 5918.300 kb on + strand at 5918.432 kb on - strand at 5918.432 kb on - strand at 5918.518 kb on - strand at 5918.518 kb on - strand at 5918.518 kb on - strand at 5918.542 kb on - strand at 5918.542 kb on - strand at 5918.553 kb on + strand at 5918.553 kb on + strand at 5918.553 kb on + strand at 5918.553 kb on + strand at 5918.554 kb on - strand at 5918.554 kb on - strand at 5918.655 kb on + strand, within Pf6N2E2_5132 at 5918.656 kb on - strand, within Pf6N2E2_5132 at 5918.664 kb on + strand, within Pf6N2E2_5132 at 5918.664 kb on + strand, within Pf6N2E2_5132 at 5918.664 kb on + strand, within Pf6N2E2_5132 at 5918.664 kb on + strand, within Pf6N2E2_5132 at 5918.665 kb on - strand, within Pf6N2E2_5132 at 5918.665 kb on - strand, within Pf6N2E2_5132 at 5918.665 kb on - strand, within Pf6N2E2_5132 at 5918.665 kb on - strand, within Pf6N2E2_5132 at 5918.665 kb on - strand, within Pf6N2E2_5132 at 5918.665 kb on - strand, within Pf6N2E2_5132 at 5918.842 kb on + strand, within Pf6N2E2_5132 at 5918.843 kb on - strand, within Pf6N2E2_5132 at 5919.062 kb on - strand, within Pf6N2E2_5132 at 5919.062 kb on - strand, within Pf6N2E2_5132 at 5919.173 kb on - strand, within Pf6N2E2_5132 at 5919.173 kb on - strand, within Pf6N2E2_5132 at 5919.173 kb on - strand, within Pf6N2E2_5132 at 5919.273 kb on + strand, within Pf6N2E2_5132
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M remove 5,915,936 + -1.3 5,915,936 + -0.5 5,916,387 + +1.0 5,916,526 + +0.9 5,916,526 + -0.2 5,916,526 + +0.5 5,916,541 + Pf6N2E2_5130 0.21 -0.5 5,916,541 + Pf6N2E2_5130 0.21 -0.5 5,916,551 + Pf6N2E2_5130 0.29 +0.5 5,916,657 - -0.5 5,916,672 + +0.6 5,916,913 - Pf6N2E2_5131 0.15 +1.0 5,916,913 - Pf6N2E2_5131 0.15 +2.1 5,916,922 + Pf6N2E2_5131 0.15 +1.0 5,917,015 + Pf6N2E2_5131 0.21 -0.1 5,917,015 + Pf6N2E2_5131 0.21 +0.5 5,917,046 - Pf6N2E2_5131 0.23 +1.7 5,917,225 + Pf6N2E2_5131 0.34 +0.3 5,917,226 - Pf6N2E2_5131 0.34 +0.8 5,917,311 + Pf6N2E2_5131 0.39 -1.8 5,917,338 + Pf6N2E2_5131 0.41 -0.7 5,917,339 - Pf6N2E2_5131 0.41 +0.6 5,917,339 - Pf6N2E2_5131 0.41 +0.3 5,918,034 + Pf6N2E2_5131 0.84 -2.0 5,918,300 + -0.0 5,918,300 + +0.1 5,918,432 - +0.1 5,918,432 - -1.0 5,918,518 - +0.2 5,918,518 - -0.1 5,918,518 - +1.2 5,918,542 - +0.7 5,918,542 - +0.8 5,918,553 + +0.2 5,918,553 + +0.7 5,918,553 + -0.2 5,918,553 + -1.8 5,918,554 - +0.4 5,918,554 - +0.8 5,918,655 + Pf6N2E2_5132 0.17 +0.7 5,918,656 - Pf6N2E2_5132 0.17 -1.0 5,918,664 + Pf6N2E2_5132 0.17 +0.4 5,918,664 + Pf6N2E2_5132 0.17 -1.4 5,918,664 + Pf6N2E2_5132 0.17 -1.0 5,918,664 + Pf6N2E2_5132 0.17 +0.0 5,918,665 - Pf6N2E2_5132 0.17 -1.0 5,918,665 - Pf6N2E2_5132 0.17 +0.3 5,918,665 - Pf6N2E2_5132 0.17 +0.6 5,918,665 - Pf6N2E2_5132 0.17 -0.8 5,918,665 - Pf6N2E2_5132 0.17 +0.0 5,918,665 - Pf6N2E2_5132 0.17 -0.3 5,918,842 + Pf6N2E2_5132 0.31 +1.3 5,918,843 - Pf6N2E2_5132 0.31 +0.2 5,919,062 - Pf6N2E2_5132 0.48 +1.0 5,919,062 - Pf6N2E2_5132 0.48 +0.4 5,919,173 - Pf6N2E2_5132 0.57 +2.2 5,919,173 - Pf6N2E2_5132 0.57 -0.7 5,919,173 - Pf6N2E2_5132 0.57 +0.1 5,919,273 + Pf6N2E2_5132 0.64 -0.3
Or see this region's nucleotide sequence