Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2758 and Pf6N2E2_2759 overlap by 32 nucleotides Pf6N2E2_2759 and Pf6N2E2_2760 overlap by 17 nucleotides Pf6N2E2_2760 and Pf6N2E2_2761 overlap by 22 nucleotides Pf6N2E2_2761 and Pf6N2E2_2762 overlap by 36 nucleotides Pf6N2E2_2762 and Pf6N2E2_2763 are separated by 35 nucleotides
Pf6N2E2_2758 - L-aspartate oxidase (EC 1.4.3.16), at 3,249,429 to 3,250,991
_2758
Pf6N2E2_2759 - FIG00955375: hypothetical protein, at 3,250,960 to 3,251,412
_2759
Pf6N2E2_2760 - YgfY COG2938, at 3,251,396 to 3,251,650
_2760
Pf6N2E2_2761 - hypothetical protein, at 3,251,629 to 3,251,835
_2761
Pf6N2E2_2762 - Folate-dependent protein for Fe/S cluster synthesis/repair in oxidative stress, at 3,251,800 to 3,252,741
_2762
Pf6N2E2_2763 - Predicted signal transduction protein, at 3,252,777 to 3,253,598
_2763
Position (kb)
3251
3252 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3250.702 kb on + strand, within Pf6N2E2_2758 at 3250.703 kb on - strand, within Pf6N2E2_2758 at 3250.722 kb on + strand, within Pf6N2E2_2758 at 3250.722 kb on + strand, within Pf6N2E2_2758 at 3250.722 kb on + strand, within Pf6N2E2_2758 at 3250.722 kb on + strand, within Pf6N2E2_2758 at 3250.723 kb on - strand, within Pf6N2E2_2758 at 3250.723 kb on - strand at 3250.806 kb on + strand, within Pf6N2E2_2758 at 3250.806 kb on + strand, within Pf6N2E2_2758 at 3250.815 kb on + strand, within Pf6N2E2_2758 at 3250.815 kb on + strand, within Pf6N2E2_2758 at 3250.815 kb on + strand, within Pf6N2E2_2758 at 3250.815 kb on + strand, within Pf6N2E2_2758 at 3250.815 kb on + strand, within Pf6N2E2_2758 at 3250.815 kb on + strand, within Pf6N2E2_2758 at 3250.815 kb on + strand, within Pf6N2E2_2758 at 3250.815 kb on + strand, within Pf6N2E2_2758 at 3250.816 kb on - strand, within Pf6N2E2_2758 at 3250.816 kb on - strand, within Pf6N2E2_2758 at 3250.816 kb on - strand, within Pf6N2E2_2758 at 3250.816 kb on - strand, within Pf6N2E2_2758 at 3250.816 kb on - strand, within Pf6N2E2_2758 at 3250.817 kb on + strand, within Pf6N2E2_2758 at 3250.817 kb on + strand, within Pf6N2E2_2758 at 3250.817 kb on + strand, within Pf6N2E2_2758 at 3250.817 kb on + strand, within Pf6N2E2_2758 at 3250.817 kb on + strand, within Pf6N2E2_2758 at 3250.818 kb on - strand, within Pf6N2E2_2758 at 3250.818 kb on - strand, within Pf6N2E2_2758 at 3250.818 kb on - strand, within Pf6N2E2_2758 at 3250.917 kb on + strand at 3250.917 kb on + strand at 3250.917 kb on + strand at 3250.917 kb on + strand at 3250.918 kb on - strand at 3250.918 kb on - strand at 3250.961 kb on + strand at 3250.962 kb on - strand at 3250.980 kb on + strand at 3250.980 kb on + strand at 3250.981 kb on - strand at 3251.187 kb on + strand, within Pf6N2E2_2759 at 3251.232 kb on - strand, within Pf6N2E2_2759 at 3251.269 kb on + strand, within Pf6N2E2_2759 at 3251.269 kb on + strand, within Pf6N2E2_2759 at 3251.269 kb on + strand, within Pf6N2E2_2759 at 3251.269 kb on + strand, within Pf6N2E2_2759 at 3251.269 kb on + strand, within Pf6N2E2_2759 at 3251.269 kb on + strand, within Pf6N2E2_2759 at 3251.269 kb on + strand, within Pf6N2E2_2759 at 3251.270 kb on - strand, within Pf6N2E2_2759 at 3251.270 kb on - strand, within Pf6N2E2_2759 at 3251.270 kb on - strand, within Pf6N2E2_2759 at 3251.270 kb on - strand, within Pf6N2E2_2759 at 3251.270 kb on - strand, within Pf6N2E2_2759 at 3251.270 kb on - strand, within Pf6N2E2_2759 at 3251.270 kb on - strand, within Pf6N2E2_2759 at 3251.270 kb on - strand, within Pf6N2E2_2759 at 3251.270 kb on - strand, within Pf6N2E2_2759 at 3251.270 kb on - strand, within Pf6N2E2_2759 at 3251.270 kb on - strand, within Pf6N2E2_2759 at 3251.348 kb on + strand, within Pf6N2E2_2759 at 3251.348 kb on + strand, within Pf6N2E2_2759 at 3251.349 kb on - strand, within Pf6N2E2_2759 at 3251.397 kb on + strand at 3251.397 kb on + strand at 3251.397 kb on + strand at 3251.398 kb on - strand at 3251.398 kb on - strand at 3251.398 kb on - strand at 3251.398 kb on - strand at 3251.398 kb on - strand at 3251.667 kb on - strand, within Pf6N2E2_2761 at 3251.667 kb on - strand, within Pf6N2E2_2761 at 3251.678 kb on - strand, within Pf6N2E2_2761 at 3251.678 kb on - strand, within Pf6N2E2_2761 at 3251.680 kb on - strand, within Pf6N2E2_2761 at 3251.680 kb on - strand, within Pf6N2E2_2761 at 3251.680 kb on - strand, within Pf6N2E2_2761 at 3251.740 kb on + strand, within Pf6N2E2_2761 at 3251.763 kb on + strand, within Pf6N2E2_2761 at 3251.763 kb on + strand, within Pf6N2E2_2761 at 3251.764 kb on - strand, within Pf6N2E2_2761 at 3251.772 kb on + strand, within Pf6N2E2_2761 at 3251.772 kb on + strand, within Pf6N2E2_2761 at 3251.784 kb on + strand, within Pf6N2E2_2761 at 3252.766 kb on - strand at 3252.766 kb on - strand at 3252.766 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 3,250,702 + Pf6N2E2_2758 0.81 +0.1 3,250,703 - Pf6N2E2_2758 0.82 -0.2 3,250,722 + Pf6N2E2_2758 0.83 -0.8 3,250,722 + Pf6N2E2_2758 0.83 -0.4 3,250,722 + Pf6N2E2_2758 0.83 -0.7 3,250,722 + Pf6N2E2_2758 0.83 -0.3 3,250,723 - Pf6N2E2_2758 0.83 -0.1 3,250,723 - +0.6 3,250,806 + Pf6N2E2_2758 0.88 +0.9 3,250,806 + Pf6N2E2_2758 0.88 -0.4 3,250,815 + Pf6N2E2_2758 0.89 +0.5 3,250,815 + Pf6N2E2_2758 0.89 +0.3 3,250,815 + Pf6N2E2_2758 0.89 -0.4 3,250,815 + Pf6N2E2_2758 0.89 +0.1 3,250,815 + Pf6N2E2_2758 0.89 -0.6 3,250,815 + Pf6N2E2_2758 0.89 -0.8 3,250,815 + Pf6N2E2_2758 0.89 -1.2 3,250,815 + Pf6N2E2_2758 0.89 +0.1 3,250,816 - Pf6N2E2_2758 0.89 -1.2 3,250,816 - Pf6N2E2_2758 0.89 -0.6 3,250,816 - Pf6N2E2_2758 0.89 -0.3 3,250,816 - Pf6N2E2_2758 0.89 +0.4 3,250,816 - Pf6N2E2_2758 0.89 +0.1 3,250,817 + Pf6N2E2_2758 0.89 -1.1 3,250,817 + Pf6N2E2_2758 0.89 +0.5 3,250,817 + Pf6N2E2_2758 0.89 -2.0 3,250,817 + Pf6N2E2_2758 0.89 +1.9 3,250,817 + Pf6N2E2_2758 0.89 +0.3 3,250,818 - Pf6N2E2_2758 0.89 -0.0 3,250,818 - Pf6N2E2_2758 0.89 +1.3 3,250,818 - Pf6N2E2_2758 0.89 +1.8 3,250,917 + +0.4 3,250,917 + -0.1 3,250,917 + +0.8 3,250,917 + +0.9 3,250,918 - +0.7 3,250,918 - -0.7 3,250,961 + +0.1 3,250,962 - -0.6 3,250,980 + +0.6 3,250,980 + -1.1 3,250,981 - +0.5 3,251,187 + Pf6N2E2_2759 0.50 -1.3 3,251,232 - Pf6N2E2_2759 0.60 -1.4 3,251,269 + Pf6N2E2_2759 0.68 +0.2 3,251,269 + Pf6N2E2_2759 0.68 -0.2 3,251,269 + Pf6N2E2_2759 0.68 +0.7 3,251,269 + Pf6N2E2_2759 0.68 -2.3 3,251,269 + Pf6N2E2_2759 0.68 +0.0 3,251,269 + Pf6N2E2_2759 0.68 -0.2 3,251,269 + Pf6N2E2_2759 0.68 +0.3 3,251,270 - Pf6N2E2_2759 0.68 +0.6 3,251,270 - Pf6N2E2_2759 0.68 +1.3 3,251,270 - Pf6N2E2_2759 0.68 -1.0 3,251,270 - Pf6N2E2_2759 0.68 +0.1 3,251,270 - Pf6N2E2_2759 0.68 +0.6 3,251,270 - Pf6N2E2_2759 0.68 +1.2 3,251,270 - Pf6N2E2_2759 0.68 -0.8 3,251,270 - Pf6N2E2_2759 0.68 -0.9 3,251,270 - Pf6N2E2_2759 0.68 +0.3 3,251,270 - Pf6N2E2_2759 0.68 +0.0 3,251,270 - Pf6N2E2_2759 0.68 -1.2 3,251,348 + Pf6N2E2_2759 0.86 +0.5 3,251,348 + Pf6N2E2_2759 0.86 -0.1 3,251,349 - Pf6N2E2_2759 0.86 -0.8 3,251,397 + +0.9 3,251,397 + -0.4 3,251,397 + -1.1 3,251,398 - -0.1 3,251,398 - -1.4 3,251,398 - -0.7 3,251,398 - -0.1 3,251,398 - -0.5 3,251,667 - Pf6N2E2_2761 0.18 -0.7 3,251,667 - Pf6N2E2_2761 0.18 -2.0 3,251,678 - Pf6N2E2_2761 0.24 -2.1 3,251,678 - Pf6N2E2_2761 0.24 -1.1 3,251,680 - Pf6N2E2_2761 0.25 -1.7 3,251,680 - Pf6N2E2_2761 0.25 -0.8 3,251,680 - Pf6N2E2_2761 0.25 -0.7 3,251,740 + Pf6N2E2_2761 0.54 -1.4 3,251,763 + Pf6N2E2_2761 0.65 -0.1 3,251,763 + Pf6N2E2_2761 0.65 -0.9 3,251,764 - Pf6N2E2_2761 0.65 +0.9 3,251,772 + Pf6N2E2_2761 0.69 +0.1 3,251,772 + Pf6N2E2_2761 0.69 +0.1 3,251,784 + Pf6N2E2_2761 0.75 +0.2 3,252,766 - +0.1 3,252,766 - -0.2 3,252,766 - -0.3
Or see this region's nucleotide sequence