Experiment: Chloride 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1026 and Pf6N2E2_1027 overlap by 4 nucleotides Pf6N2E2_1027 and Pf6N2E2_1028 are separated by 195 nucleotides
Pf6N2E2_1026 - Rhodanese-related sulfurtransferase, at 1,199,480 to 1,201,063
_1026
Pf6N2E2_1027 - Cysteine dioxygenase (EC 1.13.11.20), at 1,201,060 to 1,201,695
_1027
Pf6N2E2_1028 - Transcriptional regulator, LysR family, at 1,201,891 to 1,202,796
_1028
Position (kb)
1201
1202 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1200.219 kb on + strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.686 kb on - strand, within Pf6N2E2_1026 at 1200.808 kb on - strand, within Pf6N2E2_1026 at 1200.808 kb on - strand, within Pf6N2E2_1026 at 1200.808 kb on - strand, within Pf6N2E2_1026 at 1200.903 kb on + strand, within Pf6N2E2_1026 at 1201.052 kb on + strand at 1201.053 kb on - strand at 1201.064 kb on - strand at 1201.157 kb on + strand, within Pf6N2E2_1027 at 1201.481 kb on + strand, within Pf6N2E2_1027 at 1201.481 kb on + strand, within Pf6N2E2_1027 at 1201.481 kb on + strand, within Pf6N2E2_1027 at 1201.482 kb on - strand at 1201.482 kb on - strand, within Pf6N2E2_1027 at 1201.832 kb on + strand at 1201.864 kb on - strand at 1201.864 kb on - strand at 1201.864 kb on - strand at 1202.449 kb on + strand, within Pf6N2E2_1028 at 1202.449 kb on + strand, within Pf6N2E2_1028 at 1202.449 kb on + strand, within Pf6N2E2_1028
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 0.5 M remove 1,200,219 + Pf6N2E2_1026 0.47 +0.1 1,200,220 - Pf6N2E2_1026 0.47 +0.5 1,200,220 - Pf6N2E2_1026 0.47 +0.4 1,200,220 - Pf6N2E2_1026 0.47 +0.2 1,200,220 - Pf6N2E2_1026 0.47 -0.0 1,200,220 - Pf6N2E2_1026 0.47 -0.1 1,200,220 - Pf6N2E2_1026 0.47 -1.5 1,200,220 - Pf6N2E2_1026 0.47 -1.2 1,200,686 - Pf6N2E2_1026 0.76 +0.4 1,200,808 - Pf6N2E2_1026 0.84 +1.7 1,200,808 - Pf6N2E2_1026 0.84 -1.8 1,200,808 - Pf6N2E2_1026 0.84 -3.0 1,200,903 + Pf6N2E2_1026 0.90 -0.3 1,201,052 + +1.2 1,201,053 - -0.6 1,201,064 - +1.7 1,201,157 + Pf6N2E2_1027 0.15 -0.5 1,201,481 + Pf6N2E2_1027 0.66 +1.0 1,201,481 + Pf6N2E2_1027 0.66 +1.5 1,201,481 + Pf6N2E2_1027 0.66 +0.7 1,201,482 - +1.7 1,201,482 - Pf6N2E2_1027 0.66 +0.7 1,201,832 + +0.7 1,201,864 - -0.7 1,201,864 - -0.4 1,201,864 - -0.3 1,202,449 + Pf6N2E2_1028 0.62 -0.3 1,202,449 + Pf6N2E2_1028 0.62 +0.9 1,202,449 + Pf6N2E2_1028 0.62 -0.8
Or see this region's nucleotide sequence