Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1140 and Pf6N2E2_1141 are separated by 1 nucleotides Pf6N2E2_1141 and Pf6N2E2_1142 are separated by 49 nucleotides Pf6N2E2_1142 and Pf6N2E2_1143 are separated by 292 nucleotides
Pf6N2E2_1140 - Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-), at 1,336,764 to 1,337,507
_1140
Pf6N2E2_1141 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 1,337,509 to 1,337,802
_1141
Pf6N2E2_1142 - Polymyxin resistance protein ArnT, undecaprenyl phosphate-alpha-L-Ara4N transferase; Melittin resistance protein PqaB, at 1,337,852 to 1,339,327
_1142
Pf6N2E2_1143 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases, at 1,339,620 to 1,341,293
_1143
Position (kb)
1337
1338
1339
1340 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1336.921 kb on + strand, within Pf6N2E2_1140 at 1336.922 kb on - strand, within Pf6N2E2_1140 at 1336.922 kb on - strand, within Pf6N2E2_1140 at 1337.001 kb on + strand, within Pf6N2E2_1140 at 1337.590 kb on + strand, within Pf6N2E2_1141 at 1337.600 kb on - strand, within Pf6N2E2_1141 at 1337.600 kb on - strand, within Pf6N2E2_1141 at 1337.671 kb on + strand, within Pf6N2E2_1141 at 1337.671 kb on + strand, within Pf6N2E2_1141 at 1337.672 kb on - strand, within Pf6N2E2_1141 at 1337.856 kb on + strand at 1337.856 kb on + strand at 1337.856 kb on + strand at 1337.993 kb on + strand at 1338.071 kb on + strand, within Pf6N2E2_1142 at 1338.072 kb on - strand, within Pf6N2E2_1142 at 1338.072 kb on - strand, within Pf6N2E2_1142 at 1338.116 kb on + strand, within Pf6N2E2_1142 at 1338.116 kb on + strand, within Pf6N2E2_1142 at 1338.194 kb on + strand, within Pf6N2E2_1142 at 1338.194 kb on + strand, within Pf6N2E2_1142 at 1338.194 kb on + strand, within Pf6N2E2_1142 at 1338.257 kb on + strand, within Pf6N2E2_1142 at 1338.257 kb on + strand, within Pf6N2E2_1142 at 1338.257 kb on + strand, within Pf6N2E2_1142 at 1338.258 kb on - strand, within Pf6N2E2_1142 at 1338.258 kb on - strand, within Pf6N2E2_1142 at 1338.258 kb on - strand, within Pf6N2E2_1142 at 1338.302 kb on + strand, within Pf6N2E2_1142 at 1338.303 kb on - strand, within Pf6N2E2_1142 at 1338.305 kb on + strand, within Pf6N2E2_1142 at 1338.305 kb on + strand, within Pf6N2E2_1142 at 1338.306 kb on - strand, within Pf6N2E2_1142 at 1338.306 kb on - strand, within Pf6N2E2_1142 at 1338.369 kb on + strand, within Pf6N2E2_1142 at 1338.389 kb on + strand, within Pf6N2E2_1142 at 1338.389 kb on + strand, within Pf6N2E2_1142 at 1338.542 kb on + strand, within Pf6N2E2_1142 at 1338.542 kb on + strand, within Pf6N2E2_1142 at 1338.543 kb on - strand, within Pf6N2E2_1142 at 1338.543 kb on - strand, within Pf6N2E2_1142 at 1338.543 kb on - strand, within Pf6N2E2_1142 at 1338.581 kb on + strand, within Pf6N2E2_1142 at 1338.617 kb on + strand, within Pf6N2E2_1142 at 1338.645 kb on - strand, within Pf6N2E2_1142 at 1338.645 kb on - strand, within Pf6N2E2_1142 at 1338.794 kb on + strand, within Pf6N2E2_1142 at 1338.794 kb on + strand, within Pf6N2E2_1142 at 1338.794 kb on + strand, within Pf6N2E2_1142 at 1338.794 kb on + strand, within Pf6N2E2_1142 at 1338.795 kb on - strand, within Pf6N2E2_1142 at 1338.795 kb on - strand, within Pf6N2E2_1142 at 1338.795 kb on - strand, within Pf6N2E2_1142 at 1338.797 kb on - strand, within Pf6N2E2_1142 at 1338.797 kb on - strand, within Pf6N2E2_1142 at 1338.797 kb on - strand, within Pf6N2E2_1142 at 1339.029 kb on + strand, within Pf6N2E2_1142 at 1339.219 kb on + strand at 1339.220 kb on - strand at 1339.326 kb on - strand at 1339.571 kb on + strand at 1339.572 kb on - strand at 1339.954 kb on - strand, within Pf6N2E2_1143 at 1339.954 kb on - strand, within Pf6N2E2_1143 at 1340.130 kb on + strand, within Pf6N2E2_1143 at 1340.286 kb on + strand, within Pf6N2E2_1143 at 1340.287 kb on - strand, within Pf6N2E2_1143 at 1340.287 kb on - strand, within Pf6N2E2_1143
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 1,336,921 + Pf6N2E2_1140 0.21 +0.3 1,336,922 - Pf6N2E2_1140 0.21 +0.7 1,336,922 - Pf6N2E2_1140 0.21 +0.1 1,337,001 + Pf6N2E2_1140 0.32 +0.5 1,337,590 + Pf6N2E2_1141 0.28 -1.5 1,337,600 - Pf6N2E2_1141 0.31 +0.7 1,337,600 - Pf6N2E2_1141 0.31 -1.5 1,337,671 + Pf6N2E2_1141 0.55 -0.5 1,337,671 + Pf6N2E2_1141 0.55 +0.4 1,337,672 - Pf6N2E2_1141 0.55 +2.1 1,337,856 + -0.5 1,337,856 + +0.3 1,337,856 + -1.5 1,337,993 + -0.3 1,338,071 + Pf6N2E2_1142 0.15 -1.7 1,338,072 - Pf6N2E2_1142 0.15 +0.7 1,338,072 - Pf6N2E2_1142 0.15 -1.8 1,338,116 + Pf6N2E2_1142 0.18 -1.3 1,338,116 + Pf6N2E2_1142 0.18 -0.9 1,338,194 + Pf6N2E2_1142 0.23 +1.1 1,338,194 + Pf6N2E2_1142 0.23 -0.3 1,338,194 + Pf6N2E2_1142 0.23 -0.8 1,338,257 + Pf6N2E2_1142 0.27 +0.7 1,338,257 + Pf6N2E2_1142 0.27 -0.7 1,338,257 + Pf6N2E2_1142 0.27 -1.0 1,338,258 - Pf6N2E2_1142 0.28 -0.3 1,338,258 - Pf6N2E2_1142 0.28 -0.3 1,338,258 - Pf6N2E2_1142 0.28 -0.2 1,338,302 + Pf6N2E2_1142 0.30 +0.7 1,338,303 - Pf6N2E2_1142 0.31 +1.0 1,338,305 + Pf6N2E2_1142 0.31 -0.4 1,338,305 + Pf6N2E2_1142 0.31 +0.7 1,338,306 - Pf6N2E2_1142 0.31 -0.7 1,338,306 - Pf6N2E2_1142 0.31 +1.1 1,338,369 + Pf6N2E2_1142 0.35 +1.7 1,338,389 + Pf6N2E2_1142 0.36 -1.6 1,338,389 + Pf6N2E2_1142 0.36 -0.7 1,338,542 + Pf6N2E2_1142 0.47 -0.2 1,338,542 + Pf6N2E2_1142 0.47 -0.7 1,338,543 - Pf6N2E2_1142 0.47 +0.5 1,338,543 - Pf6N2E2_1142 0.47 +0.4 1,338,543 - Pf6N2E2_1142 0.47 +2.5 1,338,581 + Pf6N2E2_1142 0.49 +0.4 1,338,617 + Pf6N2E2_1142 0.52 -1.4 1,338,645 - Pf6N2E2_1142 0.54 -0.0 1,338,645 - Pf6N2E2_1142 0.54 -1.5 1,338,794 + Pf6N2E2_1142 0.64 -0.6 1,338,794 + Pf6N2E2_1142 0.64 -0.2 1,338,794 + Pf6N2E2_1142 0.64 +1.1 1,338,794 + Pf6N2E2_1142 0.64 +1.1 1,338,795 - Pf6N2E2_1142 0.64 -0.9 1,338,795 - Pf6N2E2_1142 0.64 -0.3 1,338,795 - Pf6N2E2_1142 0.64 -1.4 1,338,797 - Pf6N2E2_1142 0.64 -0.9 1,338,797 - Pf6N2E2_1142 0.64 +0.4 1,338,797 - Pf6N2E2_1142 0.64 -0.9 1,339,029 + Pf6N2E2_1142 0.80 -0.3 1,339,219 + -1.1 1,339,220 - +2.7 1,339,326 - -2.7 1,339,571 + -1.2 1,339,572 - -0.7 1,339,954 - Pf6N2E2_1143 0.20 +0.7 1,339,954 - Pf6N2E2_1143 0.20 -0.0 1,340,130 + Pf6N2E2_1143 0.30 +1.2 1,340,286 + Pf6N2E2_1143 0.40 -0.5 1,340,287 - Pf6N2E2_1143 0.40 +1.1 1,340,287 - Pf6N2E2_1143 0.40 -0.2
Or see this region's nucleotide sequence