Experiment: Succinic Acid; Light intensity 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0281 and Rru_A0282 are separated by 172 nucleotides Rru_A0282 and Rru_A0283 are separated by 280 nucleotides Rru_A0283 and Rru_A0284 are separated by 33 nucleotides
Rru_A0281: Rru_A0281 - Queuosine biosynthesis protein (NCBI), at 345,720 to 346,757
_A0281
Rru_A0282: Rru_A0282 - Protein of unknown function DUF204 (NCBI), at 346,930 to 347,511
_A0282
Rru_A0283: Rru_A0283 - hypothetical protein (NCBI), at 347,792 to 348,295
_A0283
Rru_A0284: Rru_A0284 - acetolactate synthase (NCBI), at 348,329 to 350,059
_A0284
Position (kb)
346
347
348 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 346.109 kb on + strand, within Rru_A0281 at 346.218 kb on + strand, within Rru_A0281 at 346.218 kb on + strand, within Rru_A0281 at 346.218 kb on + strand, within Rru_A0281 at 346.218 kb on + strand, within Rru_A0281 at 346.219 kb on - strand, within Rru_A0281 at 346.219 kb on - strand, within Rru_A0281 at 346.219 kb on - strand, within Rru_A0281 at 346.262 kb on - strand, within Rru_A0281 at 346.310 kb on + strand, within Rru_A0281 at 346.312 kb on + strand, within Rru_A0281 at 346.312 kb on + strand, within Rru_A0281 at 346.313 kb on - strand, within Rru_A0281 at 346.313 kb on - strand, within Rru_A0281 at 346.329 kb on - strand, within Rru_A0281 at 346.329 kb on - strand, within Rru_A0281 at 346.329 kb on - strand, within Rru_A0281 at 346.336 kb on + strand, within Rru_A0281 at 346.337 kb on - strand, within Rru_A0281 at 346.337 kb on - strand, within Rru_A0281 at 346.601 kb on + strand, within Rru_A0281 at 346.867 kb on - strand at 347.100 kb on + strand, within Rru_A0282 at 347.100 kb on + strand, within Rru_A0282 at 347.100 kb on + strand, within Rru_A0282 at 347.387 kb on + strand, within Rru_A0282 at 347.387 kb on + strand, within Rru_A0282 at 347.535 kb on + strand at 347.536 kb on - strand at 347.716 kb on + strand at 347.716 kb on + strand at 347.718 kb on + strand at 347.718 kb on + strand at 347.718 kb on + strand at 347.718 kb on + strand at 347.718 kb on + strand at 347.718 kb on + strand at 347.718 kb on + strand at 347.718 kb on + strand at 347.718 kb on + strand at 347.719 kb on - strand at 347.719 kb on - strand at 347.719 kb on - strand at 347.719 kb on - strand at 347.719 kb on - strand at 347.719 kb on - strand at 347.719 kb on - strand at 347.719 kb on - strand at 347.719 kb on - strand at 347.719 kb on - strand at 347.719 kb on - strand at 347.719 kb on - strand at 347.891 kb on + strand, within Rru_A0283 at 347.891 kb on + strand, within Rru_A0283 at 347.891 kb on + strand, within Rru_A0283 at 347.892 kb on - strand, within Rru_A0283 at 348.212 kb on + strand, within Rru_A0283 at 348.212 kb on + strand, within Rru_A0283 at 348.212 kb on + strand, within Rru_A0283 at 348.212 kb on + strand, within Rru_A0283 at 348.212 kb on + strand, within Rru_A0283 at 348.212 kb on + strand, within Rru_A0283 at 348.213 kb on - strand, within Rru_A0283 at 348.213 kb on - strand, within Rru_A0283 at 348.213 kb on - strand, within Rru_A0283 at 348.213 kb on - strand, within Rru_A0283 at 348.273 kb on + strand at 348.273 kb on + strand at 348.274 kb on - strand at 348.349 kb on + strand at 348.406 kb on + strand at 348.461 kb on + strand at 348.461 kb on + strand at 348.461 kb on + strand at 348.461 kb on + strand at 348.462 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 150 uM remove 346,109 + Rru_A0281 0.37 +0.2 346,218 + Rru_A0281 0.48 -1.8 346,218 + Rru_A0281 0.48 +0.2 346,218 + Rru_A0281 0.48 -1.6 346,218 + Rru_A0281 0.48 -1.5 346,219 - Rru_A0281 0.48 +0.2 346,219 - Rru_A0281 0.48 -1.8 346,219 - Rru_A0281 0.48 -3.3 346,262 - Rru_A0281 0.52 +0.2 346,310 + Rru_A0281 0.57 -2.1 346,312 + Rru_A0281 0.57 +0.2 346,312 + Rru_A0281 0.57 -1.1 346,313 - Rru_A0281 0.57 -2.8 346,313 - Rru_A0281 0.57 +0.6 346,329 - Rru_A0281 0.59 -2.3 346,329 - Rru_A0281 0.59 -1.9 346,329 - Rru_A0281 0.59 -1.6 346,336 + Rru_A0281 0.59 +0.2 346,337 - Rru_A0281 0.59 +1.2 346,337 - Rru_A0281 0.59 -1.9 346,601 + Rru_A0281 0.85 -1.8 346,867 - -1.4 347,100 + Rru_A0282 0.29 -0.9 347,100 + Rru_A0282 0.29 -1.4 347,100 + Rru_A0282 0.29 -0.8 347,387 + Rru_A0282 0.79 -2.7 347,387 + Rru_A0282 0.79 -2.6 347,535 + +0.2 347,536 - +0.2 347,716 + -1.4 347,716 + +0.2 347,718 + -0.8 347,718 + -0.8 347,718 + -0.4 347,718 + +0.2 347,718 + -2.2 347,718 + +0.2 347,718 + -1.4 347,718 + -3.4 347,718 + -1.1 347,719 - -1.1 347,719 - -2.1 347,719 - -1.3 347,719 - -1.4 347,719 - -1.8 347,719 - -1.4 347,719 - +0.2 347,719 - +0.8 347,719 - +0.2 347,719 - +0.2 347,719 - -1.1 347,719 - -1.0 347,891 + Rru_A0283 0.20 -0.2 347,891 + Rru_A0283 0.20 -0.7 347,891 + Rru_A0283 0.20 -1.6 347,892 - Rru_A0283 0.20 +0.2 348,212 + Rru_A0283 0.83 +1.2 348,212 + Rru_A0283 0.83 +1.8 348,212 + Rru_A0283 0.83 +0.2 348,212 + Rru_A0283 0.83 +0.2 348,212 + Rru_A0283 0.83 +0.2 348,212 + Rru_A0283 0.83 -0.8 348,213 - Rru_A0283 0.84 +0.2 348,213 - Rru_A0283 0.84 -0.8 348,213 - Rru_A0283 0.84 +0.2 348,213 - Rru_A0283 0.84 -1.4 348,273 + -2.3 348,273 + +0.2 348,274 - +0.2 348,349 + +0.2 348,406 + -1.8 348,461 + +0.2 348,461 + -0.8 348,461 + -1.3 348,461 + -1.8 348,462 - +1.2
Or see this region's nucleotide sequence