Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5533 and Pf1N1B4_5534 are separated by 395 nucleotides
Pf1N1B4_5533 - cobS protein, putative, at 295,253 to 296,158
_5533
Pf1N1B4_5534 - hypothetical protein, at 296,554 to 296,670
_5534
Position (kb) 295
296
297 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 294.445 kb on + strand at 294.448 kb on + strand at 294.448 kb on + strand at 294.448 kb on + strand at 294.448 kb on + strand at 294.476 kb on + strand at 294.479 kb on + strand at 294.479 kb on + strand at 294.479 kb on + strand at 294.479 kb on + strand at 294.479 kb on + strand at 294.479 kb on + strand at 294.514 kb on + strand at 294.575 kb on + strand at 294.609 kb on + strand at 294.609 kb on + strand at 294.610 kb on - strand at 294.736 kb on - strand at 294.809 kb on + strand at 294.809 kb on + strand at 294.810 kb on - strand at 294.834 kb on - strand at 294.869 kb on + strand at 294.989 kb on + strand at 294.995 kb on + strand at 294.995 kb on + strand at 294.995 kb on + strand at 294.995 kb on + strand at 294.996 kb on - strand at 294.996 kb on - strand at 294.996 kb on - strand at 295.020 kb on - strand at 295.192 kb on + strand at 295.205 kb on - strand at 295.205 kb on - strand at 295.229 kb on - strand at 295.229 kb on - strand at 295.479 kb on - strand, within Pf1N1B4_5533 at 295.660 kb on + strand, within Pf1N1B4_5533 at 295.661 kb on - strand, within Pf1N1B4_5533 at 295.819 kb on + strand, within Pf1N1B4_5533 at 295.946 kb on - strand, within Pf1N1B4_5533 at 295.962 kb on + strand, within Pf1N1B4_5533 at 295.962 kb on + strand, within Pf1N1B4_5533 at 295.963 kb on - strand, within Pf1N1B4_5533 at 296.057 kb on - strand, within Pf1N1B4_5533 at 296.199 kb on + strand at 296.199 kb on + strand at 296.199 kb on + strand at 296.199 kb on + strand at 296.200 kb on - strand at 296.200 kb on - strand at 296.200 kb on - strand at 296.200 kb on - strand at 296.200 kb on - strand at 296.200 kb on - strand at 296.200 kb on - strand at 296.291 kb on + strand at 296.304 kb on + strand at 296.304 kb on + strand at 296.334 kb on + strand at 296.552 kb on - strand at 296.692 kb on + strand at 296.693 kb on - strand at 296.745 kb on + strand at 296.745 kb on + strand at 296.745 kb on + strand at 296.904 kb on - strand at 296.914 kb on + strand at 296.932 kb on + strand at 296.932 kb on + strand at 297.054 kb on - strand at 297.088 kb on + strand at 297.110 kb on + strand at 297.111 kb on - strand at 297.114 kb on + strand at 297.115 kb on - strand at 297.146 kb on - strand at 297.148 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 294,445 + -1.6 294,448 + -1.2 294,448 + -1.5 294,448 + -0.3 294,448 + -1.4 294,476 + -2.2 294,479 + -0.8 294,479 + +0.8 294,479 + -0.6 294,479 + -0.3 294,479 + -1.5 294,479 + -0.8 294,514 + +0.8 294,575 + +0.5 294,609 + +2.0 294,609 + +0.8 294,610 - +0.8 294,736 - -0.9 294,809 + -2.1 294,809 + -0.4 294,810 - +0.5 294,834 - +1.1 294,869 + +0.8 294,989 + -3.1 294,995 + +0.9 294,995 + -0.4 294,995 + -1.7 294,995 + +0.8 294,996 - -0.9 294,996 - -2.4 294,996 - +0.1 295,020 - +0.8 295,192 + -0.2 295,205 - +0.8 295,205 - -0.1 295,229 - -1.2 295,229 - +1.6 295,479 - Pf1N1B4_5533 0.25 -0.2 295,660 + Pf1N1B4_5533 0.45 +1.7 295,661 - Pf1N1B4_5533 0.45 +0.3 295,819 + Pf1N1B4_5533 0.62 +0.8 295,946 - Pf1N1B4_5533 0.76 -1.0 295,962 + Pf1N1B4_5533 0.78 +1.0 295,962 + Pf1N1B4_5533 0.78 +0.8 295,963 - Pf1N1B4_5533 0.78 -0.8 296,057 - Pf1N1B4_5533 0.89 -0.9 296,199 + +0.8 296,199 + -1.9 296,199 + +0.8 296,199 + -0.2 296,200 - -0.5 296,200 - +0.8 296,200 - +0.4 296,200 - +0.8 296,200 - +1.6 296,200 - +0.8 296,200 - +0.3 296,291 + -1.4 296,304 + +0.8 296,304 + -0.1 296,334 + -1.8 296,552 - +0.8 296,692 + -1.6 296,693 - +1.5 296,745 + -2.5 296,745 + -0.2 296,745 + +0.8 296,904 - +3.1 296,914 + +1.0 296,932 + +0.1 296,932 + -1.5 297,054 - -3.5 297,088 + +0.8 297,110 + -0.3 297,111 - -3.0 297,114 + -2.5 297,115 - -2.9 297,146 - -0.2 297,148 + +0.8
Or see this region's nucleotide sequence