Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_460 and Pf6N2E2_461 are separated by 290 nucleotides Pf6N2E2_461 and Pf6N2E2_462 are separated by 41 nucleotides
Pf6N2E2_460 - Glycerate kinase (EC 2.7.1.31), at 524,208 to 525,344
_460
Pf6N2E2_461 - Methyl-accepting chemotaxis protein, at 525,635 to 527,260
_461
Pf6N2E2_462 - Aspartate aminotransferase (EC 2.6.1.1), at 527,302 to 528,486
_462
Position (kb)
525
526
527
528 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 525.006 kb on - strand, within Pf6N2E2_460 at 525.207 kb on + strand, within Pf6N2E2_460 at 525.207 kb on + strand, within Pf6N2E2_460 at 525.207 kb on + strand, within Pf6N2E2_460 at 525.208 kb on - strand, within Pf6N2E2_460 at 525.361 kb on - strand at 525.361 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.576 kb on - strand at 525.718 kb on - strand at 525.938 kb on + strand, within Pf6N2E2_461 at 525.977 kb on + strand, within Pf6N2E2_461 at 525.977 kb on + strand, within Pf6N2E2_461 at 525.977 kb on + strand, within Pf6N2E2_461 at 525.977 kb on + strand, within Pf6N2E2_461 at 525.978 kb on - strand, within Pf6N2E2_461 at 525.978 kb on - strand, within Pf6N2E2_461 at 525.978 kb on - strand, within Pf6N2E2_461 at 525.978 kb on - strand, within Pf6N2E2_461 at 526.007 kb on + strand, within Pf6N2E2_461 at 526.007 kb on + strand, within Pf6N2E2_461 at 526.007 kb on + strand, within Pf6N2E2_461 at 526.007 kb on + strand, within Pf6N2E2_461 at 526.082 kb on - strand, within Pf6N2E2_461 at 526.188 kb on - strand, within Pf6N2E2_461 at 526.188 kb on - strand, within Pf6N2E2_461 at 526.213 kb on + strand, within Pf6N2E2_461 at 526.213 kb on + strand, within Pf6N2E2_461 at 526.213 kb on + strand, within Pf6N2E2_461 at 526.214 kb on - strand, within Pf6N2E2_461 at 527.263 kb on + strand at 527.264 kb on - strand at 527.264 kb on - strand at 527.264 kb on - strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.300 kb on - strand at 527.300 kb on - strand at 527.300 kb on - strand at 527.300 kb on - strand at 527.300 kb on - strand at 527.300 kb on - strand at 527.300 kb on - strand at 527.494 kb on + strand, within Pf6N2E2_462 at 527.532 kb on + strand, within Pf6N2E2_462 at 527.533 kb on - strand, within Pf6N2E2_462 at 527.591 kb on + strand, within Pf6N2E2_462 at 527.617 kb on + strand, within Pf6N2E2_462 at 527.617 kb on + strand, within Pf6N2E2_462 at 527.617 kb on + strand, within Pf6N2E2_462 at 527.618 kb on - strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.921 kb on - strand, within Pf6N2E2_462 at 527.935 kb on + strand, within Pf6N2E2_462 at 527.936 kb on - strand, within Pf6N2E2_462 at 527.936 kb on - strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.188 kb on - strand, within Pf6N2E2_462
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 525,006 - Pf6N2E2_460 0.70 +0.6 525,207 + Pf6N2E2_460 0.88 -0.2 525,207 + Pf6N2E2_460 0.88 -0.2 525,207 + Pf6N2E2_460 0.88 -1.7 525,208 - Pf6N2E2_460 0.88 +2.8 525,361 - +3.0 525,361 - -0.4 525,566 - -1.0 525,566 - -0.1 525,566 - -2.2 525,566 - +0.9 525,566 - -1.8 525,566 - +1.5 525,566 - -2.4 525,566 - +2.2 525,566 - -2.6 525,576 - +1.0 525,718 - +1.8 525,938 + Pf6N2E2_461 0.19 -1.5 525,977 + Pf6N2E2_461 0.21 +0.1 525,977 + Pf6N2E2_461 0.21 +1.0 525,977 + Pf6N2E2_461 0.21 +2.1 525,977 + Pf6N2E2_461 0.21 +3.7 525,978 - Pf6N2E2_461 0.21 +0.8 525,978 - Pf6N2E2_461 0.21 -0.2 525,978 - Pf6N2E2_461 0.21 +1.5 525,978 - Pf6N2E2_461 0.21 -1.2 526,007 + Pf6N2E2_461 0.23 +1.2 526,007 + Pf6N2E2_461 0.23 -0.4 526,007 + Pf6N2E2_461 0.23 -2.8 526,007 + Pf6N2E2_461 0.23 +1.0 526,082 - Pf6N2E2_461 0.27 +2.6 526,188 - Pf6N2E2_461 0.34 +1.9 526,188 - Pf6N2E2_461 0.34 +0.0 526,213 + Pf6N2E2_461 0.36 +0.6 526,213 + Pf6N2E2_461 0.36 +2.4 526,213 + Pf6N2E2_461 0.36 -1.5 526,214 - Pf6N2E2_461 0.36 +1.5 527,263 + +0.0 527,264 - +0.6 527,264 - +0.3 527,264 - -1.2 527,299 + -2.2 527,299 + -1.0 527,299 + -1.2 527,299 + +2.3 527,299 + +2.8 527,299 + +1.2 527,299 + +1.7 527,299 + +1.8 527,299 + -0.4 527,299 + +0.9 527,300 - -2.1 527,300 - -2.4 527,300 - +0.0 527,300 - -1.0 527,300 - +1.2 527,300 - -2.1 527,300 - -0.8 527,494 + Pf6N2E2_462 0.16 +0.9 527,532 + Pf6N2E2_462 0.19 -1.5 527,533 - Pf6N2E2_462 0.19 +1.3 527,591 + Pf6N2E2_462 0.24 +1.8 527,617 + Pf6N2E2_462 0.27 -2.9 527,617 + Pf6N2E2_462 0.27 -3.1 527,617 + Pf6N2E2_462 0.27 -1.3 527,618 - Pf6N2E2_462 0.27 -0.4 527,677 + Pf6N2E2_462 0.32 +0.5 527,677 + Pf6N2E2_462 0.32 +1.3 527,677 + Pf6N2E2_462 0.32 -0.6 527,677 + Pf6N2E2_462 0.32 +0.3 527,677 + Pf6N2E2_462 0.32 +0.2 527,677 + Pf6N2E2_462 0.32 +1.5 527,677 + Pf6N2E2_462 0.32 +1.5 527,677 + Pf6N2E2_462 0.32 -1.5 527,678 - Pf6N2E2_462 0.32 -0.7 527,678 - Pf6N2E2_462 0.32 +1.1 527,678 - Pf6N2E2_462 0.32 +1.5 527,678 - Pf6N2E2_462 0.32 -1.2 527,678 - Pf6N2E2_462 0.32 +1.3 527,678 - Pf6N2E2_462 0.32 -0.2 527,678 - Pf6N2E2_462 0.32 -1.5 527,678 - Pf6N2E2_462 0.32 -1.0 527,678 - Pf6N2E2_462 0.32 +1.9 527,686 + Pf6N2E2_462 0.32 -2.5 527,686 + Pf6N2E2_462 0.32 -3.1 527,686 + Pf6N2E2_462 0.32 -1.4 527,686 + Pf6N2E2_462 0.32 +0.3 527,686 + Pf6N2E2_462 0.32 -2.0 527,686 + Pf6N2E2_462 0.32 -0.1 527,686 + Pf6N2E2_462 0.32 -0.7 527,687 - Pf6N2E2_462 0.32 -3.0 527,687 - Pf6N2E2_462 0.32 +0.5 527,687 - Pf6N2E2_462 0.32 +1.5 527,687 - Pf6N2E2_462 0.32 +0.5 527,687 - Pf6N2E2_462 0.32 +1.4 527,687 - Pf6N2E2_462 0.32 -0.1 527,687 - Pf6N2E2_462 0.32 +1.1 527,687 - Pf6N2E2_462 0.32 -0.4 527,921 - Pf6N2E2_462 0.52 -1.4 527,935 + Pf6N2E2_462 0.53 -0.8 527,936 - Pf6N2E2_462 0.54 -1.3 527,936 - Pf6N2E2_462 0.54 -2.0 528,096 + Pf6N2E2_462 0.67 -0.5 528,096 + Pf6N2E2_462 0.67 +0.6 528,096 + Pf6N2E2_462 0.67 +0.4 528,096 + Pf6N2E2_462 0.67 +1.0 528,096 + Pf6N2E2_462 0.67 +1.5 528,097 - Pf6N2E2_462 0.67 -2.7 528,097 - Pf6N2E2_462 0.67 -0.9 528,097 - Pf6N2E2_462 0.67 -0.1 528,097 - Pf6N2E2_462 0.67 -0.4 528,097 - Pf6N2E2_462 0.67 +1.6 528,097 - Pf6N2E2_462 0.67 -2.2 528,097 - Pf6N2E2_462 0.67 +1.0 528,188 - Pf6N2E2_462 0.75 +0.2
Or see this region's nucleotide sequence