Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4479

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4478 and Pf1N1B4_4479 are separated by 0 nucleotidesPf1N1B4_4479 and Pf1N1B4_4480 are separated by 1 nucleotides Pf1N1B4_4478 - Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168), at 4,939,179 to 4,940,450 _4478 Pf1N1B4_4479 - Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4), at 4,940,451 to 4,941,509 _4479 Pf1N1B4_4480 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 4,941,511 to 4,942,746 _4480 Position (kb) 4940 4941 4942Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4939.787 kb on - strandat 4939.933 kb on + strand, within Pf1N1B4_4478at 4939.934 kb on - strand, within Pf1N1B4_4478at 4939.934 kb on - strand, within Pf1N1B4_4478at 4940.195 kb on + strand, within Pf1N1B4_4478at 4940.432 kb on + strandat 4940.432 kb on + strandat 4940.433 kb on - strandat 4940.506 kb on + strandat 4940.575 kb on + strand, within Pf1N1B4_4479at 4940.773 kb on + strand, within Pf1N1B4_4479at 4940.774 kb on - strandat 4940.774 kb on - strand, within Pf1N1B4_4479at 4940.774 kb on - strand, within Pf1N1B4_4479at 4940.788 kb on + strand, within Pf1N1B4_4479at 4940.789 kb on - strand, within Pf1N1B4_4479at 4940.851 kb on + strand, within Pf1N1B4_4479at 4940.852 kb on - strand, within Pf1N1B4_4479at 4940.852 kb on - strand, within Pf1N1B4_4479at 4940.935 kb on + strand, within Pf1N1B4_4479at 4940.935 kb on + strand, within Pf1N1B4_4479at 4941.010 kb on + strand, within Pf1N1B4_4479at 4941.095 kb on - strand, within Pf1N1B4_4479at 4941.209 kb on - strand, within Pf1N1B4_4479at 4941.323 kb on - strand, within Pf1N1B4_4479at 4941.367 kb on + strand, within Pf1N1B4_4479at 4941.563 kb on + strandat 4941.782 kb on + strand, within Pf1N1B4_4480at 4941.970 kb on - strand, within Pf1N1B4_4480at 4942.235 kb on + strand, within Pf1N1B4_4480at 4942.427 kb on + strand, within Pf1N1B4_4480

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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4,939,787 - +1.3
4,939,933 + Pf1N1B4_4478 0.59 +0.8
4,939,934 - Pf1N1B4_4478 0.59 +0.7
4,939,934 - Pf1N1B4_4478 0.59 -1.6
4,940,195 + Pf1N1B4_4478 0.80 +0.5
4,940,432 + -1.4
4,940,432 + -0.9
4,940,433 - +0.5
4,940,506 + -0.1
4,940,575 + Pf1N1B4_4479 0.12 -0.6
4,940,773 + Pf1N1B4_4479 0.30 +2.3
4,940,774 - +0.5
4,940,774 - Pf1N1B4_4479 0.31 +0.5
4,940,774 - Pf1N1B4_4479 0.31 -3.7
4,940,788 + Pf1N1B4_4479 0.32 +2.0
4,940,789 - Pf1N1B4_4479 0.32 -1.5
4,940,851 + Pf1N1B4_4479 0.38 -1.2
4,940,852 - Pf1N1B4_4479 0.38 -1.2
4,940,852 - Pf1N1B4_4479 0.38 +0.8
4,940,935 + Pf1N1B4_4479 0.46 +0.6
4,940,935 + Pf1N1B4_4479 0.46 +2.0
4,941,010 + Pf1N1B4_4479 0.53 -1.9
4,941,095 - Pf1N1B4_4479 0.61 +0.5
4,941,209 - Pf1N1B4_4479 0.72 +1.2
4,941,323 - Pf1N1B4_4479 0.82 +0.0
4,941,367 + Pf1N1B4_4479 0.86 +0.8
4,941,563 + +0.5
4,941,782 + Pf1N1B4_4480 0.22 -1.9
4,941,970 - Pf1N1B4_4480 0.37 +0.5
4,942,235 + Pf1N1B4_4480 0.59 +0.5
4,942,427 + Pf1N1B4_4480 0.74 -0.7

Or see this region's nucleotide sequence