Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1361 and GFF1362 are separated by 46 nucleotides GFF1362 and GFF1363 overlap by 4 nucleotides GFF1363 and GFF1364 are separated by 97 nucleotides
GFF1361 - Nucleoprotein/polynucleotide-associated enzyme, at 15,021 to 15,560
GFF1361
GFF1362 - Ribosomal large subunit pseudouridine synthase F (EC 4.2.1.70), at 15,607 to 16,476
GFF1362
GFF1363 - hypothetical protein, at 16,473 to 16,745
GFF1363
GFF1364 - Sodium-dependent transporter, at 16,843 to 17,784
GFF1364
Position (kb) 16
17 Strain fitness (log2 ratio) -2
-1
0
1
2 at 15.559 kb on - strand at 15.559 kb on - strand at 15.583 kb on - strand at 15.606 kb on + strand at 15.640 kb on + strand at 15.640 kb on + strand at 15.640 kb on + strand at 15.642 kb on + strand at 15.806 kb on + strand, within GFF1362 at 15.806 kb on + strand, within GFF1362 at 15.948 kb on - strand, within GFF1362 at 16.107 kb on + strand, within GFF1362 at 16.180 kb on - strand, within GFF1362 at 16.537 kb on + strand, within GFF1363 at 16.601 kb on + strand, within GFF1363 at 16.610 kb on - strand, within GFF1363 at 17.107 kb on - strand, within GFF1364 at 17.151 kb on + strand, within GFF1364 at 17.152 kb on - strand, within GFF1364 at 17.455 kb on + strand, within GFF1364 at 17.456 kb on - strand, within GFF1364 at 17.563 kb on + strand, within GFF1364 at 17.661 kb on + strand, within GFF1364 at 17.662 kb on - strand, within GFF1364 at 17.720 kb on + strand at 17.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 15,559 - +1.0 15,559 - +2.6 15,583 - +0.4 15,606 + +0.1 15,640 + -0.3 15,640 + -0.6 15,640 + +2.5 15,642 + +0.4 15,806 + GFF1362 0.23 -0.1 15,806 + GFF1362 0.23 -0.0 15,948 - GFF1362 0.39 +0.0 16,107 + GFF1362 0.57 -0.4 16,180 - GFF1362 0.66 -0.3 16,537 + GFF1363 0.23 +0.2 16,601 + GFF1363 0.47 -1.1 16,610 - GFF1363 0.50 -2.6 17,107 - GFF1364 0.28 +0.1 17,151 + GFF1364 0.33 -1.0 17,152 - GFF1364 0.33 -0.2 17,455 + GFF1364 0.65 -0.1 17,456 - GFF1364 0.65 +0.2 17,563 + GFF1364 0.76 -0.1 17,661 + GFF1364 0.87 +0.2 17,662 - GFF1364 0.87 +0.1 17,720 + +1.8 17,723 + -0.1
Or see this region's nucleotide sequence