Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4732 and GFF4733 are separated by 384 nucleotides GFF4733 and GFF4734 are separated by 344 nucleotides GFF4734 and GFF4735 are separated by 86 nucleotides
GFF4732 - SPI1-associated transcriptional regulator SprB, at 40,750 to 41,505
GFF4732
GFF4733 - Type III secretion transcriptional regulator HilC (= SirC), at 41,890 to 42,777
GFF4733
GFF4734 - Putative effector protein OrgC of SPI-1 type III secretion system, at 43,122 to 43,484
GFF4734
GFF4735 - OrgB protein, associated with InvC ATPase of type III secretion system, at 43,571 to 44,251
GFF4735
Position (kb) 41
42
43 Strain fitness (log2 ratio) -1
0
1 at 41.098 kb on - strand, within GFF4732 at 41.748 kb on - strand at 41.784 kb on - strand at 41.787 kb on + strand at 41.787 kb on + strand at 42.284 kb on + strand, within GFF4733 at 43.026 kb on + strand at 43.070 kb on + strand at 43.081 kb on - strand at 43.082 kb on + strand at 43.085 kb on - strand at 43.085 kb on - strand at 43.097 kb on - strand at 43.365 kb on - strand, within GFF4734 at 43.476 kb on - strand at 43.546 kb on + strand at 43.547 kb on - strand at 43.616 kb on - strand at 43.682 kb on - strand, within GFF4735 at 43.754 kb on - strand, within GFF4735
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 41,098 - GFF4732 0.46 -0.4 41,748 - +0.1 41,784 - +0.5 41,787 + -0.1 41,787 + -0.2 42,284 + GFF4733 0.44 +0.9 43,026 + +1.1 43,070 + -0.0 43,081 - +0.0 43,082 + -0.7 43,085 - -1.4 43,085 - -0.1 43,097 - -0.2 43,365 - GFF4734 0.67 +0.5 43,476 - +0.3 43,546 + -0.1 43,547 - +0.4 43,616 - -1.6 43,682 - GFF4735 0.16 -0.2 43,754 - GFF4735 0.27 -0.3
Or see this region's nucleotide sequence