Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10250 and BWI76_RS10255 overlap by 17 nucleotides BWI76_RS10255 and BWI76_RS10260 are separated by 10 nucleotides BWI76_RS10260 and BWI76_RS10265 overlap by 14 nucleotides
BWI76_RS10250: BWI76_RS10250 - glutamine amidotransferase, at 2,170,877 to 2,171,974
_RS10250
BWI76_RS10255: BWI76_RS10255 - dihydrodipicolinate synthase family protein, at 2,171,958 to 2,172,881
_RS10255
BWI76_RS10260: BWI76_RS10260 - creatinase, at 2,172,892 to 2,174,061
_RS10260
BWI76_RS10265: BWI76_RS10265 - mandelate racemase/muconate lactonizing protein, at 2,174,048 to 2,175,148
_RS10265
Position (kb)
2172
2173
2174
2175 Strain fitness (log2 ratio)
-1
0
1 at 2171.900 kb on - strand at 2172.023 kb on - strand at 2172.023 kb on - strand at 2172.023 kb on - strand at 2172.668 kb on - strand, within BWI76_RS10255 at 2172.750 kb on + strand, within BWI76_RS10255 at 2172.750 kb on + strand, within BWI76_RS10255 at 2173.688 kb on + strand, within BWI76_RS10260 at 2173.696 kb on - strand, within BWI76_RS10260 at 2173.966 kb on + strand at 2173.974 kb on - strand at 2173.974 kb on - strand at 2174.069 kb on - strand at 2174.399 kb on + strand, within BWI76_RS10265 at 2174.485 kb on - strand, within BWI76_RS10265 at 2174.485 kb on - strand, within BWI76_RS10265 at 2174.799 kb on - strand, within BWI76_RS10265 at 2174.854 kb on - strand, within BWI76_RS10265 at 2174.904 kb on - strand, within BWI76_RS10265
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 2,171,900 - +0.0 2,172,023 - +0.3 2,172,023 - -0.0 2,172,023 - -0.1 2,172,668 - BWI76_RS10255 0.77 -0.4 2,172,750 + BWI76_RS10255 0.86 -0.1 2,172,750 + BWI76_RS10255 0.86 -0.4 2,173,688 + BWI76_RS10260 0.68 +0.7 2,173,696 - BWI76_RS10260 0.69 +0.3 2,173,966 + -0.1 2,173,974 - -0.8 2,173,974 - +0.8 2,174,069 - +1.2 2,174,399 + BWI76_RS10265 0.32 +0.1 2,174,485 - BWI76_RS10265 0.40 +0.1 2,174,485 - BWI76_RS10265 0.40 -0.0 2,174,799 - BWI76_RS10265 0.68 -0.7 2,174,854 - BWI76_RS10265 0.73 +0.2 2,174,904 - BWI76_RS10265 0.78 +0.1
Or see this region's nucleotide sequence