Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2559 and Dsui_2560 are separated by 98 nucleotides Dsui_2560 and Dsui_2561 overlap by 4 nucleotides Dsui_2561 and Dsui_2562 are separated by 17 nucleotides Dsui_2562 and Dsui_2563 are separated by 3 nucleotides
Dsui_2559: Dsui_2559 - Protein of unknown function (DUF2523), at 2,710,055 to 2,710,408
_2559
Dsui_2560: Dsui_2560 - zonula occludens toxin, at 2,710,507 to 2,711,397
_2560
Dsui_2561: Dsui_2561 - type II secretory pathway, component PulD, at 2,711,394 to 2,712,605
_2561
Dsui_2562: Dsui_2562 - hypothetical protein, at 2,712,623 to 2,712,862
_2562
Dsui_2563: Dsui_2563 - hypothetical protein, at 2,712,866 to 2,713,087
_2563
Position (kb)
2711
2712
2713 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2710.414 kb on + strand at 2710.447 kb on - strand at 2710.546 kb on - strand at 2710.596 kb on + strand at 2710.769 kb on + strand, within Dsui_2560 at 2710.896 kb on - strand at 2710.973 kb on - strand, within Dsui_2560 at 2710.983 kb on - strand, within Dsui_2560 at 2711.031 kb on - strand, within Dsui_2560 at 2711.040 kb on + strand at 2711.048 kb on - strand at 2711.131 kb on - strand at 2711.131 kb on - strand, within Dsui_2560 at 2711.214 kb on - strand, within Dsui_2560 at 2711.408 kb on + strand at 2711.427 kb on + strand at 2711.500 kb on + strand at 2711.567 kb on + strand, within Dsui_2561 at 2711.622 kb on - strand, within Dsui_2561 at 2711.680 kb on - strand, within Dsui_2561 at 2711.738 kb on + strand at 2711.819 kb on + strand, within Dsui_2561 at 2711.866 kb on + strand, within Dsui_2561 at 2712.089 kb on + strand, within Dsui_2561 at 2712.089 kb on + strand, within Dsui_2561 at 2712.094 kb on + strand, within Dsui_2561 at 2712.285 kb on - strand, within Dsui_2561 at 2712.328 kb on + strand, within Dsui_2561 at 2712.336 kb on - strand, within Dsui_2561 at 2712.433 kb on - strand, within Dsui_2561 at 2712.466 kb on + strand, within Dsui_2561 at 2712.496 kb on + strand at 2712.554 kb on + strand at 2712.556 kb on - strand at 2712.608 kb on + strand at 2712.608 kb on + strand at 2712.620 kb on + strand at 2712.628 kb on - strand at 2712.677 kb on - strand at 2712.730 kb on + strand at 2712.776 kb on - strand, within Dsui_2562 at 2712.783 kb on + strand, within Dsui_2562 at 2712.783 kb on + strand, within Dsui_2562 at 2712.788 kb on + strand, within Dsui_2562 at 2712.788 kb on + strand, within Dsui_2562 at 2712.940 kb on + strand, within Dsui_2563 at 2712.948 kb on - strand, within Dsui_2563 at 2712.965 kb on + strand, within Dsui_2563 at 2712.973 kb on - strand, within Dsui_2563 at 2712.973 kb on - strand, within Dsui_2563 at 2713.041 kb on - strand, within Dsui_2563 at 2713.043 kb on + strand, within Dsui_2563 at 2713.057 kb on - strand, within Dsui_2563 at 2713.061 kb on - strand, within Dsui_2563 at 2713.081 kb on + strand at 2713.082 kb on + strand at 2713.103 kb on - strand at 2713.120 kb on - strand at 2713.140 kb on + strand at 2713.174 kb on + strand at 2713.182 kb on - strand at 2713.205 kb on + strand at 2713.232 kb on - strand at 2713.253 kb on + strand at 2713.305 kb on + strand at 2713.322 kb on - strand at 2713.333 kb on + strand at 2713.357 kb on - strand at 2713.357 kb on - strand at 2713.462 kb on + strand at 2713.462 kb on + strand at 2713.472 kb on + strand at 2713.487 kb on - strand at 2713.530 kb on + strand at 2713.530 kb on + strand at 2713.537 kb on + strand at 2713.542 kb on + strand at 2713.542 kb on + strand at 2713.542 kb on + strand at 2713.542 kb on + strand at 2713.542 kb on + strand at 2713.542 kb on + strand at 2713.542 kb on + strand at 2713.544 kb on + strand at 2713.544 kb on + strand at 2713.548 kb on + strand at 2713.550 kb on + strand at 2713.550 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,710,414 + -0.8 2,710,447 - -0.8 2,710,546 - +0.8 2,710,596 + +0.8 2,710,769 + Dsui_2560 0.29 -0.2 2,710,896 - +0.8 2,710,973 - Dsui_2560 0.52 +0.8 2,710,983 - Dsui_2560 0.53 +0.8 2,711,031 - Dsui_2560 0.59 -1.8 2,711,040 + +0.8 2,711,048 - +0.8 2,711,131 - -0.2 2,711,131 - Dsui_2560 0.70 +0.8 2,711,214 - Dsui_2560 0.79 +0.8 2,711,408 + +0.8 2,711,427 + -0.2 2,711,500 + +0.8 2,711,567 + Dsui_2561 0.14 +2.2 2,711,622 - Dsui_2561 0.19 -0.2 2,711,680 - Dsui_2561 0.24 +0.8 2,711,738 + +0.8 2,711,819 + Dsui_2561 0.35 +0.8 2,711,866 + Dsui_2561 0.39 +0.8 2,712,089 + Dsui_2561 0.57 +0.8 2,712,089 + Dsui_2561 0.57 -0.8 2,712,094 + Dsui_2561 0.58 +0.8 2,712,285 - Dsui_2561 0.74 +0.8 2,712,328 + Dsui_2561 0.77 -1.2 2,712,336 - Dsui_2561 0.78 +0.8 2,712,433 - Dsui_2561 0.86 -2.0 2,712,466 + Dsui_2561 0.88 -1.2 2,712,496 + -1.2 2,712,554 + +0.8 2,712,556 - +0.8 2,712,608 + -0.2 2,712,608 + +0.8 2,712,620 + -0.2 2,712,628 - +0.8 2,712,677 - +0.8 2,712,730 + +0.8 2,712,776 - Dsui_2562 0.64 +0.8 2,712,783 + Dsui_2562 0.67 -1.8 2,712,783 + Dsui_2562 0.67 -0.2 2,712,788 + Dsui_2562 0.69 -0.2 2,712,788 + Dsui_2562 0.69 +0.8 2,712,940 + Dsui_2563 0.33 +0.8 2,712,948 - Dsui_2563 0.37 +0.8 2,712,965 + Dsui_2563 0.45 +0.8 2,712,973 - Dsui_2563 0.48 +0.8 2,712,973 - Dsui_2563 0.48 +0.8 2,713,041 - Dsui_2563 0.79 -0.8 2,713,043 + Dsui_2563 0.80 -1.2 2,713,057 - Dsui_2563 0.86 -0.2 2,713,061 - Dsui_2563 0.88 +0.8 2,713,081 + -0.2 2,713,082 + -0.8 2,713,103 - +0.8 2,713,120 - +0.8 2,713,140 + +0.8 2,713,174 + +0.8 2,713,182 - -0.2 2,713,205 + +0.8 2,713,232 - +0.8 2,713,253 + +0.8 2,713,305 + +0.8 2,713,322 - -1.2 2,713,333 + +0.8 2,713,357 - -1.2 2,713,357 - -0.2 2,713,462 + -1.2 2,713,462 + +0.8 2,713,472 + -0.8 2,713,487 - -0.2 2,713,530 + +0.9 2,713,530 + -0.1 2,713,537 + -0.0 2,713,542 + +0.2 2,713,542 + +1.6 2,713,542 + +0.8 2,713,542 + -1.4 2,713,542 + -1.0 2,713,542 + -0.6 2,713,542 + +0.2 2,713,544 + -0.2 2,713,544 + -0.8 2,713,548 + +0.7 2,713,550 + -0.1 2,713,550 - +0.8
Or see this region's nucleotide sequence