Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0497 and Dsui_0498 are separated by 16 nucleotides Dsui_0498 and Dsui_0499 are separated by 27 nucleotides Dsui_0499 and Dsui_0500 are separated by 31 nucleotides Dsui_0500 and Dsui_0501 are separated by 5 nucleotides
Dsui_0497: Dsui_0497 - TIGR00255 family protein, at 499,496 to 500,362
_0497
Dsui_0498: Dsui_0498 - guanylate kinase, at 500,379 to 500,987
_0498
Dsui_0499: Dsui_0499 - DNA-directed RNA polymerase, omega subunit, at 501,015 to 501,221
_0499
Dsui_0500: Dsui_0500 - (p)ppGpp synthetase, RelA/SpoT family, at 501,253 to 503,484
_0500
Dsui_0501: Dsui_0501 - glycine/D-amino acid oxidase, deaminating, at 503,490 to 504,740
_0501
Position (kb) 501
502
503
504 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 500.287 kb on - strand at 500.293 kb on + strand at 501.006 kb on + strand at 501.035 kb on + strand at 501.040 kb on + strand, within Dsui_0499 at 501.134 kb on - strand, within Dsui_0499 at 501.215 kb on + strand at 501.230 kb on - strand at 502.502 kb on - strand, within Dsui_0500 at 502.502 kb on - strand, within Dsui_0500 at 503.002 kb on + strand, within Dsui_0500 at 503.066 kb on + strand, within Dsui_0500
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 500,287 - +0.4 500,293 + -1.0 501,006 + -1.5 501,035 + -0.3 501,040 + Dsui_0499 0.12 +0.8 501,134 - Dsui_0499 0.57 -0.6 501,215 + +3.4 501,230 - +0.9 502,502 - Dsui_0500 0.56 +3.3 502,502 - Dsui_0500 0.56 +2.9 503,002 + Dsui_0500 0.78 +5.0 503,066 + Dsui_0500 0.81 +5.3
Or see this region's nucleotide sequence