Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0012 and Dsui_0013 are separated by 1 nucleotides Dsui_0013 and Dsui_0014 are separated by 496 nucleotides Dsui_0014 and Dsui_0015 are separated by 134 nucleotides Dsui_0015 and Dsui_0016 are separated by 39 nucleotides
Dsui_0012: Dsui_0012 - putative permease, DMT superfamily, at 10,877 to 11,764
_0012
Dsui_0013: Dsui_0013 - Thioredoxin, at 11,766 to 12,266
_0013
Dsui_0014: Dsui_0014 - putative membrane protein, at 12,763 to 13,377
_0014
Dsui_0015: Dsui_0015 - putative signal-transduction protein containing cAMP-binding and CBS domains, at 13,512 to 13,943
_0015
Dsui_0016: Dsui_0016 - transcriptional regulator, at 13,983 to 14,891
_0016
Position (kb) 12
13
14 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 11.767 kb on - strand at 11.772 kb on - strand at 11.951 kb on + strand at 11.983 kb on - strand, within Dsui_0013 at 12.306 kb on + strand at 12.374 kb on + strand at 12.604 kb on - strand at 12.609 kb on - strand at 12.711 kb on - strand at 12.781 kb on - strand at 12.781 kb on - strand at 12.929 kb on + strand, within Dsui_0014 at 12.935 kb on + strand, within Dsui_0014 at 12.950 kb on + strand, within Dsui_0014 at 12.954 kb on - strand, within Dsui_0014 at 12.954 kb on - strand at 13.011 kb on + strand, within Dsui_0014 at 13.040 kb on + strand, within Dsui_0014 at 13.040 kb on + strand, within Dsui_0014 at 13.048 kb on - strand at 13.048 kb on - strand, within Dsui_0014 at 13.127 kb on - strand at 13.223 kb on - strand, within Dsui_0014 at 13.225 kb on - strand at 13.238 kb on + strand, within Dsui_0014 at 13.253 kb on + strand, within Dsui_0014 at 13.289 kb on + strand, within Dsui_0014 at 13.297 kb on - strand, within Dsui_0014 at 13.308 kb on - strand, within Dsui_0014 at 13.357 kb on - strand at 13.457 kb on + strand at 13.510 kb on - strand at 13.522 kb on - strand at 13.525 kb on + strand at 13.525 kb on + strand at 13.598 kb on - strand, within Dsui_0015 at 13.609 kb on + strand, within Dsui_0015 at 13.678 kb on + strand, within Dsui_0015 at 13.678 kb on + strand at 13.696 kb on + strand at 13.786 kb on + strand, within Dsui_0015 at 13.786 kb on - strand at 13.853 kb on - strand at 13.935 kb on + strand at 14.245 kb on - strand, within Dsui_0016 at 14.266 kb on - strand, within Dsui_0016 at 14.346 kb on + strand at 14.370 kb on - strand, within Dsui_0016
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 11,767 - -1.5 11,772 - -0.4 11,951 + +0.9 11,983 - Dsui_0013 0.43 +0.9 12,306 + +0.9 12,374 + +0.9 12,604 - +0.9 12,609 - -2.8 12,711 - -0.1 12,781 - -0.1 12,781 - -1.1 12,929 + Dsui_0014 0.27 +3.3 12,935 + Dsui_0014 0.28 +2.8 12,950 + Dsui_0014 0.30 +2.4 12,954 - Dsui_0014 0.31 -1.1 12,954 - +0.9 13,011 + Dsui_0014 0.40 +3.1 13,040 + Dsui_0014 0.45 +2.5 13,040 + Dsui_0014 0.45 +2.8 13,048 - -0.1 13,048 - Dsui_0014 0.46 -0.1 13,127 - -0.1 13,223 - Dsui_0014 0.75 -0.1 13,225 - +0.9 13,238 + Dsui_0014 0.77 +2.4 13,253 + Dsui_0014 0.80 +2.8 13,289 + Dsui_0014 0.86 +2.6 13,297 - Dsui_0014 0.87 -1.1 13,308 - Dsui_0014 0.89 -0.7 13,357 - +0.9 13,457 + +0.9 13,510 - +0.9 13,522 - -0.7 13,525 + -0.7 13,525 + +0.9 13,598 - Dsui_0015 0.20 -0.1 13,609 + Dsui_0015 0.22 +0.9 13,678 + Dsui_0015 0.38 -0.1 13,678 + +0.9 13,696 + -0.1 13,786 + Dsui_0015 0.63 -1.5 13,786 - +0.9 13,853 - -0.1 13,935 + -1.1 14,245 - Dsui_0016 0.29 +0.1 14,266 - Dsui_0016 0.31 +0.5 14,346 + +0.9 14,370 - Dsui_0016 0.43 +0.4
Or see this region's nucleotide sequence