Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4769 and GFF4770 are separated by 72 nucleotides GFF4770 and GFF4771 are separated by 411 nucleotides GFF4771 and GFF4772 are separated by 530 nucleotides GFF4772 and GFF4773 are separated by 207 nucleotides
GFF4769 - FIG001353: Acetyltransferase, at 77,405 to 77,929
GFF4769
GFF4770 - transposase, at 78,002 to 78,142
GFF4770
GFF4771 - Serine/threonine specific protein phosphatase 2 (EC 3.1.3.16), at 78,554 to 79,210
GFF4771
GFF4772 - hypothetical protein, at 79,741 to 79,854
GFF4772
GFF4773 - DNA mismatch repair protein MutS, at 80,062 to 82,629
GFF4773
Position (kb)
78
79
80 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 77.610 kb on + strand, within GFF4769 at 77.610 kb on + strand, within GFF4769 at 77.612 kb on + strand, within GFF4769 at 77.654 kb on + strand, within GFF4769 at 77.655 kb on - strand, within GFF4769 at 77.702 kb on - strand, within GFF4769 at 77.790 kb on + strand, within GFF4769 at 77.790 kb on + strand, within GFF4769 at 77.926 kb on - strand at 77.950 kb on + strand at 78.005 kb on + strand at 78.005 kb on + strand at 78.094 kb on - strand, within GFF4770 at 78.840 kb on + strand, within GFF4771 at 78.852 kb on - strand, within GFF4771 at 78.995 kb on + strand, within GFF4771 at 79.033 kb on - strand, within GFF4771 at 79.227 kb on + strand at 79.274 kb on + strand at 79.275 kb on - strand at 79.577 kb on + strand at 79.697 kb on + strand at 79.716 kb on + strand at 79.788 kb on + strand, within GFF4772 at 79.906 kb on + strand at 79.907 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 77,610 + GFF4769 0.39 -0.0 77,610 + GFF4769 0.39 +0.2 77,612 + GFF4769 0.39 +0.3 77,654 + GFF4769 0.47 -1.1 77,655 - GFF4769 0.48 -2.7 77,702 - GFF4769 0.57 -1.1 77,790 + GFF4769 0.73 +0.7 77,790 + GFF4769 0.73 -1.4 77,926 - -0.1 77,950 + +0.5 78,005 + +0.4 78,005 + -2.9 78,094 - GFF4770 0.65 -0.0 78,840 + GFF4771 0.44 -0.2 78,852 - GFF4771 0.45 -0.3 78,995 + GFF4771 0.67 -0.9 79,033 - GFF4771 0.73 -0.7 79,227 + +0.8 79,274 + +1.8 79,275 - +0.7 79,577 + -0.3 79,697 + +0.6 79,716 + +1.5 79,788 + GFF4772 0.41 -1.9 79,906 + +0.6 79,907 - -0.3
Or see this region's nucleotide sequence