Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3559 and GFF3560 are separated by 16 nucleotides GFF3560 and GFF3561 overlap by 26 nucleotides GFF3561 and GFF3562 overlap by 4 nucleotides
GFF3559 - Iron-sulfur cluster assembly protein SufB, at 314,745 to 316,232
GFF3559
GFF3560 - Iron-sulfur cluster assembly ATPase protein SufC, at 316,249 to 316,995
GFF3560
GFF3561 - Iron-sulfur cluster assembly protein SufD, at 316,970 to 318,241
GFF3561
GFF3562 - Cysteine desulfurase (EC 2.8.1.7), SufS subfamily, at 318,238 to 319,458
GFF3562
Position (kb) 316
317
318
319 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 316.022 kb on + strand, within GFF3559 at 316.022 kb on + strand, within GFF3559 at 316.260 kb on + strand at 316.440 kb on + strand, within GFF3560 at 316.658 kb on + strand, within GFF3560 at 317.067 kb on + strand at 317.266 kb on + strand, within GFF3561 at 317.384 kb on + strand, within GFF3561 at 317.384 kb on + strand, within GFF3561 at 317.384 kb on + strand, within GFF3561 at 317.384 kb on + strand, within GFF3561 at 317.385 kb on - strand, within GFF3561 at 317.388 kb on + strand, within GFF3561 at 317.388 kb on + strand, within GFF3561 at 317.388 kb on + strand, within GFF3561 at 317.389 kb on - strand, within GFF3561 at 317.431 kb on - strand, within GFF3561 at 317.437 kb on + strand, within GFF3561 at 317.546 kb on - strand, within GFF3561 at 317.546 kb on - strand, within GFF3561 at 317.727 kb on + strand, within GFF3561 at 317.728 kb on - strand, within GFF3561 at 317.782 kb on + strand, within GFF3561 at 317.783 kb on - strand, within GFF3561 at 317.839 kb on + strand, within GFF3561 at 317.839 kb on + strand, within GFF3561 at 317.854 kb on + strand, within GFF3561 at 317.854 kb on + strand, within GFF3561 at 318.024 kb on - strand, within GFF3561 at 318.068 kb on - strand, within GFF3561 at 318.314 kb on - strand at 318.346 kb on - strand at 318.376 kb on + strand, within GFF3562 at 318.474 kb on + strand, within GFF3562 at 318.585 kb on + strand, within GFF3562 at 318.687 kb on + strand, within GFF3562 at 318.688 kb on - strand, within GFF3562 at 318.707 kb on + strand, within GFF3562 at 319.137 kb on + strand, within GFF3562 at 319.213 kb on + strand, within GFF3562 at 319.214 kb on - strand, within GFF3562
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 316,022 + GFF3559 0.86 +0.9 316,022 + GFF3559 0.86 +1.8 316,260 + -0.8 316,440 + GFF3560 0.26 -0.8 316,658 + GFF3560 0.55 +2.1 317,067 + +0.3 317,266 + GFF3561 0.23 +0.4 317,384 + GFF3561 0.33 +0.9 317,384 + GFF3561 0.33 +0.6 317,384 + GFF3561 0.33 -0.3 317,384 + GFF3561 0.33 -0.0 317,385 - GFF3561 0.33 +0.5 317,388 + GFF3561 0.33 +1.1 317,388 + GFF3561 0.33 -0.7 317,388 + GFF3561 0.33 +0.7 317,389 - GFF3561 0.33 +2.5 317,431 - GFF3561 0.36 +0.5 317,437 + GFF3561 0.37 +0.2 317,546 - GFF3561 0.45 -0.2 317,546 - GFF3561 0.45 +0.2 317,727 + GFF3561 0.60 -2.6 317,728 - GFF3561 0.60 +0.4 317,782 + GFF3561 0.64 +0.5 317,783 - GFF3561 0.64 +0.6 317,839 + GFF3561 0.68 +0.4 317,839 + GFF3561 0.68 -0.1 317,854 + GFF3561 0.69 +0.6 317,854 + GFF3561 0.69 +1.9 318,024 - GFF3561 0.83 -1.9 318,068 - GFF3561 0.86 -0.4 318,314 - +0.2 318,346 - +1.3 318,376 + GFF3562 0.11 -3.9 318,474 + GFF3562 0.19 +0.1 318,585 + GFF3562 0.28 +0.6 318,687 + GFF3562 0.37 +1.2 318,688 - GFF3562 0.37 -0.2 318,707 + GFF3562 0.38 +1.4 319,137 + GFF3562 0.74 -0.1 319,213 + GFF3562 0.80 +1.6 319,214 - GFF3562 0.80 +0.1
Or see this region's nucleotide sequence