Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3485 and GFF3486 are separated by 4 nucleotides GFF3486 and GFF3487 are separated by 29 nucleotides
GFF3485 - Selenide,water dikinase (EC 2.7.9.3), at 237,662 to 238,705
GFF3485
GFF3486 - DNA topoisomerase III (EC 5.99.1.2), at 238,710 to 240,659
GFF3486
GFF3487 - NADP-specific glutamate dehydrogenase (EC 1.4.1.4), at 240,689 to 242,032
GFF3487
Position (kb) 238
239
240
241 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 237.746 kb on - strand at 237.995 kb on - strand, within GFF3485 at 238.084 kb on + strand, within GFF3485 at 238.085 kb on - strand, within GFF3485 at 238.129 kb on - strand, within GFF3485 at 238.334 kb on - strand, within GFF3485 at 238.451 kb on + strand, within GFF3485 at 238.451 kb on + strand, within GFF3485 at 238.452 kb on - strand, within GFF3485 at 238.452 kb on - strand, within GFF3485 at 238.454 kb on - strand, within GFF3485 at 238.502 kb on + strand, within GFF3485 at 238.832 kb on + strand at 238.974 kb on - strand, within GFF3486 at 239.061 kb on + strand, within GFF3486 at 239.144 kb on + strand, within GFF3486 at 239.231 kb on + strand, within GFF3486 at 239.232 kb on - strand, within GFF3486 at 239.418 kb on - strand, within GFF3486 at 239.544 kb on + strand, within GFF3486 at 239.665 kb on + strand, within GFF3486 at 239.666 kb on - strand, within GFF3486 at 239.667 kb on + strand, within GFF3486 at 239.667 kb on + strand, within GFF3486 at 239.937 kb on + strand, within GFF3486 at 240.066 kb on - strand, within GFF3486 at 240.092 kb on + strand, within GFF3486 at 240.237 kb on - strand, within GFF3486 at 240.359 kb on + strand, within GFF3486 at 240.382 kb on + strand, within GFF3486 at 240.383 kb on - strand, within GFF3486 at 240.417 kb on - strand, within GFF3486 at 240.505 kb on + strand at 240.505 kb on + strand at 240.507 kb on + strand at 240.508 kb on - strand at 240.766 kb on + strand at 240.766 kb on + strand at 240.787 kb on - strand at 240.942 kb on - strand, within GFF3487 at 241.123 kb on - strand, within GFF3487 at 241.297 kb on - strand, within GFF3487 at 241.363 kb on + strand, within GFF3487 at 241.364 kb on - strand, within GFF3487 at 241.384 kb on - strand, within GFF3487
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 237,746 - +0.5 237,995 - GFF3485 0.32 +1.1 238,084 + GFF3485 0.40 +1.6 238,085 - GFF3485 0.41 +1.0 238,129 - GFF3485 0.45 -0.3 238,334 - GFF3485 0.64 -0.8 238,451 + GFF3485 0.76 +0.8 238,451 + GFF3485 0.76 -0.7 238,452 - GFF3485 0.76 -0.9 238,452 - GFF3485 0.76 -0.8 238,454 - GFF3485 0.76 -0.9 238,502 + GFF3485 0.80 -0.3 238,832 + +2.0 238,974 - GFF3486 0.14 -1.3 239,061 + GFF3486 0.18 +1.3 239,144 + GFF3486 0.22 -3.3 239,231 + GFF3486 0.27 -0.8 239,232 - GFF3486 0.27 +1.1 239,418 - GFF3486 0.36 +1.0 239,544 + GFF3486 0.43 -0.1 239,665 + GFF3486 0.49 +0.6 239,666 - GFF3486 0.49 -2.0 239,667 + GFF3486 0.49 -0.2 239,667 + GFF3486 0.49 -2.8 239,937 + GFF3486 0.63 +0.6 240,066 - GFF3486 0.70 -0.7 240,092 + GFF3486 0.71 -1.9 240,237 - GFF3486 0.78 -1.2 240,359 + GFF3486 0.85 +0.1 240,382 + GFF3486 0.86 +0.7 240,383 - GFF3486 0.86 -1.6 240,417 - GFF3486 0.88 +0.3 240,505 + +0.1 240,505 + +1.9 240,507 + +0.3 240,508 - +0.7 240,766 + -1.4 240,766 + -3.8 240,787 - +0.7 240,942 - GFF3487 0.19 -1.2 241,123 - GFF3487 0.32 -0.9 241,297 - GFF3487 0.45 +0.4 241,363 + GFF3487 0.50 +0.2 241,364 - GFF3487 0.50 +0.5 241,384 - GFF3487 0.52 +0.7
Or see this region's nucleotide sequence