Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF924 and GFF925 overlap by 6 nucleotides GFF925 and GFF926 are separated by 224 nucleotides GFF926 and GFF927 are separated by 115 nucleotides GFF927 and GFF928 are separated by 74 nucleotides
GFF924 - Trehalose operon transcriptional repressor, at 101,709 to 102,656
GFF924
GFF925 - hypothetical protein, at 102,651 to 102,767
GFF925
GFF926 - Mg(2+) transport ATPase, P-type (EC 3.6.3.2), at 102,992 to 105,748
GFF926
GFF927 - Mobile element protein, at 105,864 to 106,310
GFF927
GFF928 - Endoribonuclease L-PSP, at 106,385 to 106,771
GFF928
Position (kb)
102
103
104
105
106 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 101.992 kb on + strand, within GFF924 at 102.064 kb on + strand, within GFF924 at 102.065 kb on - strand, within GFF924 at 102.123 kb on - strand, within GFF924 at 102.165 kb on - strand, within GFF924 at 102.337 kb on - strand, within GFF924 at 102.368 kb on + strand, within GFF924 at 102.369 kb on - strand, within GFF924 at 102.372 kb on - strand, within GFF924 at 102.380 kb on - strand, within GFF924 at 102.380 kb on - strand, within GFF924 at 102.458 kb on + strand, within GFF924 at 102.750 kb on - strand, within GFF925 at 102.764 kb on - strand at 103.035 kb on + strand at 103.040 kb on - strand at 103.040 kb on - strand at 103.243 kb on - strand at 103.336 kb on - strand, within GFF926 at 103.603 kb on + strand, within GFF926 at 103.606 kb on + strand, within GFF926 at 103.774 kb on + strand, within GFF926 at 103.906 kb on + strand, within GFF926 at 103.918 kb on + strand, within GFF926 at 103.919 kb on - strand, within GFF926 at 103.967 kb on - strand, within GFF926 at 103.977 kb on - strand, within GFF926 at 104.155 kb on + strand, within GFF926 at 104.248 kb on - strand, within GFF926 at 104.248 kb on - strand, within GFF926 at 104.486 kb on - strand, within GFF926 at 104.510 kb on - strand, within GFF926 at 104.530 kb on + strand, within GFF926 at 104.530 kb on + strand, within GFF926 at 104.530 kb on + strand, within GFF926 at 104.607 kb on - strand, within GFF926 at 104.669 kb on + strand, within GFF926 at 104.740 kb on + strand, within GFF926 at 104.847 kb on + strand, within GFF926 at 104.847 kb on + strand, within GFF926 at 104.915 kb on - strand, within GFF926 at 105.016 kb on + strand, within GFF926 at 105.256 kb on - strand, within GFF926 at 105.260 kb on + strand, within GFF926 at 105.293 kb on - strand, within GFF926 at 105.626 kb on + strand at 105.657 kb on - strand at 105.657 kb on - strand at 105.657 kb on - strand at 105.657 kb on - strand at 105.690 kb on - strand at 105.691 kb on + strand at 105.691 kb on + strand at 105.691 kb on + strand at 105.692 kb on - strand at 105.692 kb on - strand at 105.692 kb on - strand at 105.692 kb on - strand at 105.900 kb on - strand at 106.021 kb on + strand, within GFF927 at 106.025 kb on + strand, within GFF927 at 106.039 kb on - strand, within GFF927 at 106.039 kb on - strand, within GFF927 at 106.042 kb on + strand, within GFF927 at 106.073 kb on + strand, within GFF927 at 106.074 kb on - strand, within GFF927 at 106.083 kb on - strand, within GFF927 at 106.095 kb on + strand, within GFF927 at 106.144 kb on - strand, within GFF927 at 106.153 kb on - strand, within GFF927 at 106.164 kb on + strand, within GFF927 at 106.164 kb on + strand, within GFF927 at 106.501 kb on - strand, within GFF928 at 106.501 kb on - strand, within GFF928 at 106.545 kb on - strand, within GFF928 at 106.545 kb on - strand, within GFF928 at 106.545 kb on - strand, within GFF928 at 106.595 kb on - strand, within GFF928 at 106.700 kb on - strand, within GFF928 at 106.718 kb on + strand, within GFF928 at 106.719 kb on - strand, within GFF928 at 106.722 kb on + strand, within GFF928 at 106.723 kb on - strand, within GFF928 at 106.723 kb on - strand, within GFF928
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 101,992 + GFF924 0.30 -2.3 102,064 + GFF924 0.37 -3.6 102,065 - GFF924 0.38 -2.5 102,123 - GFF924 0.44 +0.0 102,165 - GFF924 0.48 +3.3 102,337 - GFF924 0.66 +0.2 102,368 + GFF924 0.70 -0.1 102,369 - GFF924 0.70 +0.6 102,372 - GFF924 0.70 -1.8 102,380 - GFF924 0.71 -0.4 102,380 - GFF924 0.71 +1.9 102,458 + GFF924 0.79 +0.7 102,750 - GFF925 0.85 +1.4 102,764 - +0.4 103,035 + -3.6 103,040 - -0.6 103,040 - -2.7 103,243 - +0.2 103,336 - GFF926 0.12 +0.5 103,603 + GFF926 0.22 -1.7 103,606 + GFF926 0.22 -0.0 103,774 + GFF926 0.28 -0.8 103,906 + GFF926 0.33 +0.1 103,918 + GFF926 0.34 +0.7 103,919 - GFF926 0.34 +0.6 103,967 - GFF926 0.35 -1.6 103,977 - GFF926 0.36 -0.4 104,155 + GFF926 0.42 -1.5 104,248 - GFF926 0.46 +1.1 104,248 - GFF926 0.46 -0.4 104,486 - GFF926 0.54 -0.5 104,510 - GFF926 0.55 -1.9 104,530 + GFF926 0.56 -0.2 104,530 + GFF926 0.56 +0.6 104,530 + GFF926 0.56 -1.6 104,607 - GFF926 0.59 -0.6 104,669 + GFF926 0.61 -0.0 104,740 + GFF926 0.63 +0.4 104,847 + GFF926 0.67 +0.5 104,847 + GFF926 0.67 -0.2 104,915 - GFF926 0.70 +0.7 105,016 + GFF926 0.73 -0.1 105,256 - GFF926 0.82 -2.1 105,260 + GFF926 0.82 -0.1 105,293 - GFF926 0.83 +1.3 105,626 + +0.5 105,657 - +0.8 105,657 - -0.1 105,657 - -0.8 105,657 - -2.2 105,690 - +0.1 105,691 + -1.5 105,691 + +0.7 105,691 + +1.9 105,692 - -0.2 105,692 - +0.4 105,692 - -1.7 105,692 - -0.6 105,900 - +0.5 106,021 + GFF927 0.35 -2.3 106,025 + GFF927 0.36 -3.8 106,039 - GFF927 0.39 -1.0 106,039 - GFF927 0.39 -2.5 106,042 + GFF927 0.40 +0.3 106,073 + GFF927 0.47 -1.0 106,074 - GFF927 0.47 -1.7 106,083 - GFF927 0.49 +0.4 106,095 + GFF927 0.52 -0.9 106,144 - GFF927 0.63 +0.3 106,153 - GFF927 0.65 -0.7 106,164 + GFF927 0.67 +0.5 106,164 + GFF927 0.67 -2.6 106,501 - GFF928 0.30 -0.2 106,501 - GFF928 0.30 -2.5 106,545 - GFF928 0.41 -0.8 106,545 - GFF928 0.41 +0.2 106,545 - GFF928 0.41 -3.4 106,595 - GFF928 0.54 -0.8 106,700 - GFF928 0.81 +0.0 106,718 + GFF928 0.86 -1.1 106,719 - GFF928 0.86 -2.5 106,722 + GFF928 0.87 -1.7 106,723 - GFF928 0.87 +0.1 106,723 - GFF928 0.87 +2.1
Or see this region's nucleotide sequence