Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3714 and GFF3715 are separated by 51 nucleotides GFF3715 and GFF3716 overlap by 4 nucleotides
GFF3714 - Phosphoribulokinase (EC 2.7.1.19) homolog, function unknown, at 20,104 to 20,973
GFF3714
GFF3715 - FIG01199774: hypothetical protein, at 21,025 to 21,243
GFF3715
GFF3716 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 21,240 to 22,199
GFF3716
Position (kb) 21
22 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 20.151 kb on + strand at 20.151 kb on + strand at 20.152 kb on - strand at 20.194 kb on + strand, within GFF3714 at 20.195 kb on - strand, within GFF3714 at 20.195 kb on - strand, within GFF3714 at 20.199 kb on + strand, within GFF3714 at 20.397 kb on - strand, within GFF3714 at 20.508 kb on + strand, within GFF3714 at 20.508 kb on + strand, within GFF3714 at 20.632 kb on - strand, within GFF3714 at 20.672 kb on + strand, within GFF3714 at 20.673 kb on - strand, within GFF3714 at 20.673 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.688 kb on + strand, within GFF3714 at 20.703 kb on + strand, within GFF3714 at 20.724 kb on + strand, within GFF3714 at 20.730 kb on + strand, within GFF3714 at 20.731 kb on - strand, within GFF3714 at 20.731 kb on - strand, within GFF3714 at 20.779 kb on - strand, within GFF3714 at 20.835 kb on - strand, within GFF3714 at 20.945 kb on - strand at 21.002 kb on - strand at 21.002 kb on - strand at 21.002 kb on - strand at 21.007 kb on - strand at 21.070 kb on + strand, within GFF3715 at 21.162 kb on - strand, within GFF3715 at 21.259 kb on + strand at 21.413 kb on + strand, within GFF3716 at 21.414 kb on - strand, within GFF3716 at 21.437 kb on + strand, within GFF3716 at 21.437 kb on + strand, within GFF3716 at 21.437 kb on + strand, within GFF3716 at 21.482 kb on + strand, within GFF3716 at 21.483 kb on - strand, within GFF3716 at 21.493 kb on + strand, within GFF3716 at 21.493 kb on + strand, within GFF3716 at 21.494 kb on - strand, within GFF3716 at 21.497 kb on - strand, within GFF3716 at 21.648 kb on - strand, within GFF3716 at 21.664 kb on + strand, within GFF3716 at 21.695 kb on - strand, within GFF3716 at 21.698 kb on + strand, within GFF3716 at 21.698 kb on + strand, within GFF3716 at 21.788 kb on + strand, within GFF3716 at 21.788 kb on + strand, within GFF3716 at 21.792 kb on - strand, within GFF3716 at 21.892 kb on + strand, within GFF3716 at 21.892 kb on + strand, within GFF3716 at 21.897 kb on - strand, within GFF3716 at 22.047 kb on - strand, within GFF3716 at 22.049 kb on - strand, within GFF3716 at 22.167 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 20,151 + -0.5 20,151 + -0.2 20,152 - +0.3 20,194 + GFF3714 0.10 +0.9 20,195 - GFF3714 0.10 -0.2 20,195 - GFF3714 0.10 -3.0 20,199 + GFF3714 0.11 -0.5 20,397 - GFF3714 0.34 +1.6 20,508 + GFF3714 0.46 +1.4 20,508 + GFF3714 0.46 -0.5 20,632 - GFF3714 0.61 +0.4 20,672 + GFF3714 0.65 -1.1 20,673 - GFF3714 0.65 -0.0 20,673 - GFF3714 0.65 +2.0 20,677 - GFF3714 0.66 -0.8 20,677 - GFF3714 0.66 +0.3 20,677 - GFF3714 0.66 +1.2 20,677 - GFF3714 0.66 +0.5 20,677 - GFF3714 0.66 +0.2 20,677 - GFF3714 0.66 -0.7 20,688 + GFF3714 0.67 -2.4 20,703 + GFF3714 0.69 +0.2 20,724 + GFF3714 0.71 -0.3 20,730 + GFF3714 0.72 +1.8 20,731 - GFF3714 0.72 +0.7 20,731 - GFF3714 0.72 +1.3 20,779 - GFF3714 0.78 +0.4 20,835 - GFF3714 0.84 -1.6 20,945 - +1.2 21,002 - +0.3 21,002 - +0.2 21,002 - -0.7 21,007 - +0.3 21,070 + GFF3715 0.21 -1.0 21,162 - GFF3715 0.63 -2.1 21,259 + -3.0 21,413 + GFF3716 0.18 -2.0 21,414 - GFF3716 0.18 +0.1 21,437 + GFF3716 0.21 +1.5 21,437 + GFF3716 0.21 -0.2 21,437 + GFF3716 0.21 +1.5 21,482 + GFF3716 0.25 -1.7 21,483 - GFF3716 0.25 -1.1 21,493 + GFF3716 0.26 -1.2 21,493 + GFF3716 0.26 -0.5 21,494 - GFF3716 0.26 -0.8 21,497 - GFF3716 0.27 -3.8 21,648 - GFF3716 0.42 -0.3 21,664 + GFF3716 0.44 +0.0 21,695 - GFF3716 0.47 +0.0 21,698 + GFF3716 0.48 -1.5 21,698 + GFF3716 0.48 +1.7 21,788 + GFF3716 0.57 -3.4 21,788 + GFF3716 0.57 -0.3 21,792 - GFF3716 0.57 -0.1 21,892 + GFF3716 0.68 +0.3 21,892 + GFF3716 0.68 -2.6 21,897 - GFF3716 0.68 +1.2 22,047 - GFF3716 0.84 +0.3 22,049 - GFF3716 0.84 +0.3 22,167 - -0.7
Or see this region's nucleotide sequence